Substituted cyclic amine metalloprotease inhibitors

ABSTRACT

The invention provides compounds which are useful as inhibitors of metalloproteases, and which are effective in treating conditions characterized by excess activity of these enzymes. In particular, the present invention relates to a compound having a structure according to Formula (I).  
                 
 
     Also disclosed are compounds, pharmaceutical compositions and methods of treating diseases characterized by metalloprotease activity using these compounds or the pharmaceutical compositions containing them.

CROSS REFERENCE

[0001] This application claims priority under Title 35, United StatesCode 119(e) from Provisional Application Serial No. 60/024,842, filedAug. 28, 1996.

TECHNICAL FIELD

[0002] This invention is directed to compounds which are useful intreating diseases associated with metalloprotease activity, particularlyzinc metalloprotease activity.

BACKGROUND

[0003] Background

[0004] A number of structurally related metalloproteases [MPs] effectthe breakdown of structural proteins. These metalloproteases often acton the intercellular matrix, and thus are involved in tissue breakdownand remodeling. Such proteins are referred to as metalloproteases orMPs. There are several different families of MPs, classified by sequencehomology. Several families of known MPs, as well as examples thereof,are disclosed in the art.

[0005] These MPs include Matrix-Metallo Proteases [MMPs], zincmetalloproteases, many of the membrane bound metalloproteases, TNFconverting enzymes, angiotensin-converting enzymes (ACEs), disintegrins,including ADAMs (See Wolfsberg et al, 131 J. Cell Bio. 275-78 October,1995), and the enkephalinases. Examples of MPs include human skinfibroblast collagenase, human skin fibroblast gelatinase, human sputumcollagenase, aggrecanse and gelatinase, and human stromelysin.Collagenase, stromelysin, aggrecanase and related enzymes are thought tobe important in mediating the symptomatology of a number of diseases.

[0006] Potential therapeutic indications of MP inhibitors have beendiscussed in the literature. See for example, U.S. Pat. Nos. 5,506,242(Ciba Geigy Corp.); 5,403,952 (Merck & Co.); PCT published applicationWO 96106074 (British Bio Tech Ltd); PCT Publication WO 96/00214 (CibaGeigy); WO 95/35275 (British Bio Tech Ltd); WO 95/35276 (British BioTech Ltd); WO 95/33731 (Hoffman-LaRoche); WO 95/33709 (Hoffman-LaRoche);WO 95/32944 (British Bio Tech Ltd); WO 95/26989 (Merck); WO 9529892(DuPont Merck); WO 95/24921 (Inst. Opthamology); WO 95/23790 (SmithKlineBeecham); WO 95/22966 (Sanofi Winthrop); WO 95/19965 (Glycomed); WO 9519956 (British Bio Tech Ltd); WO 95/19957 (British Bio Tech Ltd); WO95/19961 (British Bio Tech Ltd) WO 95/13289 (Chiroscience Ltd.); WO95/12603 (Syntex); WO 95/09633 (Florida State Univ); WO 95/09620(Florida State Univ.); WO 95/04033 (Celltech); WO 94/25434 (Celltech);WO 94/25435 (Celltech); WO 93/14112 ( Merck); WO 94/0019 (Glaxo); WO93/21942 (British Bio Tech Ltd); WO 92/22523 (Res. Corp. Tech. Inc.); WO94/10990 (British Bio Tech Ltd); WO 93/09090 (Yamanouchi); and Britishpatents GB 2282598 (Merck) and GB 2268934 (British Bio Tech Ltd);Published European Patent Applications EP 95/684240 (Hoffman LaRoche);EP 574758 (Hoffman LaRoche); EP 575844 (Hoffman LaRoche); PublishedJapanese applications; JP 08053403 (Fujusowa Pharm. Co. Ltd.); JP7304770 (Kanebo Ltd.); and Bird et al J. Med Chem vol. 37, pp. 158-69(1994). Examples of potential therapeutic uses of MP inhibitors includerheumatoid arthritis (Mullins, D. E., et al., Biochim. Biophys. Acta.(1983) 695:117-214); osteoardhritis (Henderson, B., et al., Drugs of theFuture (1990) 15:495-508); the metastasis of tumor cells (ibid,Broadhurst, M. J., et al., European Patent Application 276,436(published 1987), Reich, R., et al., 48 Cancer Res. 3307-3312 (1988);and various ulcerations or ulcerative conditions of tissue. For example,ulcerative conditions can result in the cornea as the result of alkaliburns or as a result of infection by Pseudomonas aeruginosa,Acanthamoeba, Herpes simplex and vaccinia viruses.

[0007] Other examples of conditions characterized by undesiredmetalloprotease activity include periodontal disease, epidermolysisbullosa, fever, inflammation and scleritis (Cf. DeCicco et al, WO 9529892 published Nov. 9, 1995).

[0008] In view of the involvement of such metalloproteases in a numberof disease conditions, attempts have been made to prepare inhibitors tothese enzymes. A number of such inhibitors are disclosed in theliterature. Examples include U.S. Pat. Nos. 5,183,900, issued Feb. 2,1993 to Galardy; 4,996,358, issued Feb. 26, 1991 to Handa, et al.;4,771,038, issued Sep. 13, 1988 to Wolanin, et al.; 4,743,587, issuedMay 10, 1988 to Dickens, et al., European Patent Publication Number575,844, published Dec. 29, 1993 by Broadhurst, et al.; InternationalPatent Publication No. WO 93/09090, published May 13, 1993 by Isomura,et al.; World Patent Publication 92/17460, published Oct. 15, 1992 byMarkwell et al.; and European Patent Publication Number 498,665,published Aug. 12, 1992 by Beckett, et al.

[0009] Though a variety of inhibitors have been prepared, there is acontinuing need for potent matrix metalloprotease inhibitors useful intreating such diseases. It would be advantageous to inhibit thesemetalloproteases as a method of treating diseases related to unwantedmetalloprotease activity. Though a variety of inhibitors have beenprepared, there is a continuing need for potent metalloproteaseinhibitors useful in treating such diseases.

OBJECTS OF THE INVENTION

[0010] It is an object of the invention to provide potent inhibitors ofmetalloproteases.

[0011] It is a further object of the invention to provide pharmaceuticalcompositions comprising such inhibitors.

[0012] It is also an object of the invention to provide a method oftreatment for metalloprotease related maladies.

SUMMARY OF THE INVENTION

[0013] The invention provides compounds which are useful as inhibitorsof metalloproteases, and which are effective in treating conditionscharacterized by excess activity of these enzymes. In particular, thepresent invention relates to a compound having a structure according toFormula (I)

[0014] wherein

[0015] Ar is alkyl, heteroalkyl, aryl or heteroaryl, substituted orunsubstituted;

[0016] R₁ is OH, alkoxy, NHOR₂, where R₂ is hydrogen or alkyl;

[0017] W is one or more of hydrogen, lower alkyl or an alkylene bridge;

[0018] Y is independently one or more of hydroxy, SR₃, SOR₄, SO₂R₅,alkoxy, amino, wherein amino is of formula NR₆,R₇, wherein R₆ and R₇ areindependently chosen from hydrogen, alkyl, heteroalkyl, heteroaryl,aryl, OR₃, SO₂R₈, COR₉, CSR₁₀, PO(R₁₁)₂; and

[0019] R₃ is hydrogen, alkyl, aryl, heteroaryl;

[0020] R₄ is alkyl, aryl, heteroaryl;

[0021] R₈ is alkyl, aryl, heteroaryl, heteroalkyl, amino, alkylamino,dialkylamino, arylamino, diarylamino and alkylarylamino;

[0022] R₉ is hydrogen, alkoxy, aryloxy, heteroaryloxy, alkyl, aryl,heteroaryl, heteroalkyl, amino, alkylamino, dialkylamino, arylamino andalkylarylamino;

[0023] R₁₀ is alkyl, aryl, heteroaryl, heteroalkyl, amino, alkylamino,dialkylamino, arylamino, diarylamino and alkylarylamino;

[0024] R₁₁ is alkyl, aryl, heteroaryl, heteroalkyl;

[0025] Z is hydrogen, hydroxy, alkyl, alkylene or heteroalkylene;

[0026] n is 1-3.

[0027] This structure also includes an optical isomer, diastereomer orenantiomer for Formula (I), or a pharmaceutically-acceptable salt, orbiohydrolyzable amide, ester, or imide thereof.

[0028] These compounds have the ability to inhibit at least onemammalian matrix metalloprotease. Accordingly, in other aspects, theinvention is directed to pharmaceutical compositions containing thecompounds of Formula (I), and to methods of treating diseasescharacterized by metalloprotease activity using these compounds or thepharmaceutical compositions containing them.

[0029] Applicants have found that compounds of Formula (I) are potentinhibitors of metalloproteases. The compounds of the present inventiontherefore are useful for the treatment of conditions and diseases whichare characterized by unwanted activity by the class of proteins whichdestroy structural proteins.

[0030] Metalloproteases active at a particularly undesired location(e.g., an organ or certain types of cells) can be targeted byconjugating the compounds of the invention to a targeting ligandspecific for a marker at that location such as an antibody or fragmentthereof or a receptor ligand. Conjugation methods are known in the art.

[0031] The invention is also directed to various other processes whichtake advantage of the unique properties of these compounds. Thus, inanother aspect, the invention is directed to the compounds of Formula(I) conjugated to solid supports. These conjugates can be used asaffinity reagents for the purification of a desired metalloprotease.

[0032] In another aspect, the invention is directed to the compounds ofFormula (I) conjugated to label. As the compounds of the invention bindto at least one metalloprotease, the label can be used to detect thepresence of relatively high levels of metalloprotease in vivo or invitro cell culture.

[0033] In addition, the compounds of Formula (I) can be conjugated tocarriers which permit the use of these compounds in immunizationprotocols to prepare antibodies specifically immunoreactive with thecompounds of the invention. Typical conjugation methods are known in theart. These antibodies are then useful both in therapy and in monitoringthe dosage of the inhibitors.

DETAILED DESCRIPTION

[0034] The compounds of the present invention are inhibitors ofmammalian metalloproteases. Preferably, the compounds are those ofFormula (I) or a pharmaceutically-acceptable salt, or biohydrolyzableamide, ester, or imide thereof.

[0035] Definitions and Usage of Terms:

[0036] The following is a list of definitions for terms used herein:

[0037] “Acyl” or “carbonyl” is described as a radical which could beformed by removal of the hydroxy from a carboxylic acid (i.e.,R—C(═O)—). Preferred acyl groups include (for example) acetyl, formyl,and propionyl.

[0038] “Acyloxy” is an oxy radical having an acyl substituent (i.e.,—O-acyl); for example, —O—C(═O)-alkyl.

[0039] “Alkoxyacyl” is an acyl radical (—C(═O)—) having an alkoxysubtituent (i.e., —O—R), for example, —C(═O)—O-alkyl. This radical canbe referred to as an ester.

[0040] “Acylamino” is an amino radical having an acyl substituent (i.e.,—N-acyl); for example, —NH—C(═O)-alkyl.

[0041] “Alkenyl” is an unsubstituted or substituted hydrocarbon chainradical having 2 to 15 carbon atoms; preferably from 2 to 10 carbonatoms; more preferably from 2 to 8; except where indicated. Alkenylsubstituents have at least one olefinic double bond (including, forexample, vinyl, allyl and butenyl).

[0042] “Alkynyl” is an unsubstituted or substituted hydrocarbon chainradical having 2 to 15 carbon atoms; preferably from 2 to 10 carbonatoms; more preferably from 2 to 8; except where indicated. The chainhas at least one carbon-carbon triple bond.

[0043] “Alkoxy” is an oxygen radical having a hydrocarbon chainsubstituent, where the hydrocarbon chain is an alkyl or alkenyl (i.e.,—O-alkyl or —O-alkenyl). Preferred alkoxy groups include (for example)methoxy, ethoxy, propoxy and allyloxy.

[0044] “Alkoxyalkyl” is an unsubstituted or substituted alkyl moietysubstituted with an alkoxy moiety (i.e., -alkyl-O-alkyl). Preferred iswhere the alkyl has 1 to 6 carbon atoms (more preferably 1 to 3 carbonatoms), and the alkyoxy has 1 to 6 carbon atoms (more preferably 1 to 3carbon atoms).

[0045] “Alkyl” is an unsubstituted or substituted saturated hydrocarbonchain radical having 1 to 15 carbon atoms; preferably from 1 to 10carbon atoms; more preferably 1 to 4; except where indicated. Preferredalkyl groups include (for example) substituted or unsubstituted methyl,ethyl, propyl, isopropyl, and butyl.

[0046] Alkylene refers to an alkyl, alkenyl or alkynyl which isdiradical, rather than a radical. “Hetero alkylene” is likewise definedas a (diradical) alkylene having a heteroatom in its chain. hence an“alkylene bridge” is a hydrocarbon diradical that attaches to twodifferent carbons (hence making a bicyclic structure), prefered alkylenebridges include methylene, ethylene and propylene.

[0047] “Alkylamino” is an amino radical having one (secondary amine) ortwo (tertiary amine) alkyl substituents (i.e., —N-alkyl). For example,methylamino (—NHCH₃), dimethylamino (—N(CH₃)₂), methylethylamino(—N(CH₃)CH₂CH₃).

[0048] “Aminoacyl” is acyl radical having an amino substituent (i.e.,—C(═O)—N); for example, —C(═O)—NH₂. The amino group of the aminoacylmoiety may be unsubstituted (i.e., primary amine) or may be substitutedwith one (secondary amine) or two (i.e., tertiary amine) alkyl groups.

[0049] “Aryl” is an aromatic carbocyclic ring radical. Preferred arylgroups include (for example) phenyl, tolyl, xylyl, cumenyl and naphthyl.

[0050] “Arylalkyl” is an alkyl radical substituted with an aryl group.Preferred arylalkyl groups include benzyl, phenylethyl, andphenylpropyl.

[0051] “Arylalkylamino” is an amine radical substituted with anarylalkyl group (e.g., —NH-benzyl).

[0052] “Arylamino” is an amine radical substituted with an aryl group(i.e., —NH-aryl).

[0053] “Aryloxy” is an oxygen radical having an aryl substituent (i.e.,—O-aryl).

[0054] “Carbocyclic ring” is an unsubstituted or substituted, saturated,unsaturated or aromatic, hydrocarbon ring radical. Carbocyclic rings aremonocyclic or are fused, bridged or spiro polycyclic ring systems.Monocyclic carbocyclic rings generally contain 4 to 9 atoms, preferably4 to 7 atoms. Polycyclic carbocyclic rings contain 7 to 17 atoms,preferably from 7 to 12 atoms. Preferred polycyclic systems comprise 4-,5-, 6- or 7-membered rings fused to 5-, 6-, or 7-membered rings.

[0055] “Carbocycle-alkyl” is an unsubstituted or substituted alkylradical substituted with a carbocyclic ring. Unless otherwise specified,the carbocyclic ring is preferably an aryl or cycloalkyl; morepreferably an aryl. Preferred carbocycle-alkyl groups include benzyl,phenylethyl and phenylpropyl.

[0056] “Carbocycle-heteroalkyl” is an unsubstituted or substitutedheteroalkyl radical substituted with a carbocyclic ring. Unlessotherwise specified, the carbocyclic ring is preferably an aryl orcycloalkyl; more preferably an aryl. The heteroalkyl is preferably2-oxa-propyl, 2-oxa-ethyl, 2-thia-propyl, or 2-thia-ethyl.

[0057] “Carboxyalkyl” is an unsubstituted or substituted alkyl radicalsubstituted with with a carboxy (—C(═O)OH) moiety. For example,—CH₂—C(═O)OH.

[0058] “Cycloalkyl” is a saturated carbocyclic ring radical. Preferredcycloalkyl groups include (for example) cyclopropyl, cyclobutyl andcyclohexyl.

[0059] “Cycloheteroalkyl” is a saturated heterocyclic ring. Preferredcycloheteroalkyl groups include (for example) morpholinyl, piperadinyl,and piperazinyl.

[0060] “Fused rings” are rings that are superimposed together such thatthey share two ring atoms. A given ring may be fused to more than oneother ring. Fused rings are contemplated in heteroaryl, aryl andheterocycle radicals or the like.

[0061] “Heterocycle-alkyl” is an alkyl radical substituted with aheterocyclic ring. The heterocyclic ring is preferably an heteroaryl orcycloheteroalkyl; more preferably an heteroaryl. Preferred heterocyclealkyl include C₁-C₄ alkyl having preferred heteroaryl appended to them.More preferred is, for example, pyridyl alkyl, and the like.

[0062] “Heterocycle-heteroalkyl” is an unsubstituted or substitutedheteroalkyl radical substituted with a heterocyclic ring. Theheterocyclic ring is preferably an aryl or cycloheteroalkyl; morepreferably an aryl.

[0063] “Heteroatom” is a nitrogen, sulfur or oxygen atom. Groupscontaining one or more heteroatoms may contain different heteroatoms.

[0064] “Heteroalkenyl” is an unsubstituted or substituted unsaturatedchain radical having 3 to 8 members comprising carbon atoms and one ortwo heteroatoms. The chain has at least one carbon-carbon double bond.

[0065] “Heteroalkyl” is an unsubstituted or substituted saturated chainradical having 2 to 8 comprising carbon atoms and one or twoheteroatoms.

[0066] “Heterocyclic ring” is an unsubstituted or substituted,saturated, unsaturated or aromatic ring radical comprised of carbonatoms and one or more heteroatoms in the ring. Heterocyclic rings aremonocyclic or are fused, bridged or spiro polycyclic ring systems.Monocyclic heterocyclic rings contain 3 to 9 atoms, preferably 4 to 7atoms. Polycyclic rings contain 7 to 17 atoms, preferably from 7 to 13atoms.

[0067] “Heteroaryl” is an aromatic heterocyclic ring, either monocyclicor bicyclic radical. Preferred heteroaryl groups include (for example)thienyl, furyl, pyrrolyl, pyridinyl, pyrazinyl, thiazolyl, pyrimidinyl,quinolinyl, and tetrazolyl, benzo thiazolyl, benzofuryl, indolyl and thelike.

[0068] “Halo”, “halogen”, or “halide” includes chloro, bromo, fluoro oriodo, preferably chloro and fluoro.

[0069] Also, as referred to herein, a lower hydrocarbon moiety (e.g.,“lower” alkyl) is a hydrocarbon chain comprised of 1 to 6, preferablyfrom 1 to 4, carbon atoms.

[0070] A “pharmaceutically-acceptable salt” is a cationic salt formed atany acidic (e.g., carboxyl) group, or an anionic salt formed at anybasic (e.g., amino) group. Many such salts are known in the art, asdescribed in World Patent Publication 87/05297, Johnston et al.,published Sep. 11, 1987 (incorporated by reference herein). Preferredcationic salts include the alkali metal salts (such as sodium andpotassium), and alkaline earth metal salts (such as magnesium andcalcium) and organic salts. Preferred anionic salts include the halides(such as chloride salts). Such salts are well understood by the skilledartisan, and the skilled artisan is able to prepare any number of saltsgiven the knowledge in the art. Furthermore, it is recognized that theskilled artisan may prefer one salt over another for reasons ofsolubility, stability, formulation ease and the like. Determination andoptimization of of such salts is within the purview of the skilledartisans practice.

[0071] “Biohydrolyzable amides” are amides of a metalloproteaseinhibitor that do not interfere with the inhibitory activity of thecompound, or that are readily converted in vivo by an animal, preferablya mammal, more preferably a human subject to yield an activemetalloprotease inhibitor.

[0072] A “biohydrolyzable hydroxy imide” is an imide of a Formula (I)compound that does not interfere with the metalloprotease inhibitoryactivity of these compounds, or that is readily converted in vivo by ananimal, preferably a mammal, more preferably a human subject to yield anactive Formula (I) compound.

[0073] A “biohydrolyzable ester” refers to an ester of a Formula (I)compound that does not interfere with the metalloprotease inhibitoryactivity of these compounds or that is readily converted by an animal toyield an active Formula (I) compound.

[0074] A “solvate” is a complex formed by the combination of a solute(e.g., a metalloprotease inhibitor) and a solvent (e.g., water). See J.Honig et al., The Van Nostrand Chemist's Dictionary, p. 650 (1953).Pharmaceutically-acceptable solvents used according to this inventioninclude those that do not interfere with the biological activity of themetalloprotease inhibitor (e.g., water, ethanol, acetic acid,N,N-dimethylformamide and others known or readily determined by theskilled artisan).

[0075] “Optical isomer”, “stereoisomer”, “diastereomer” as referred toherein have the standard art recognized meanings (Cf., Hawley'sCondensed Chemical Dictionary, 11th Ed.).

[0076] The illustration of specific protected forms and otherderivatives of the Formula (I) compounds is not intended to be limiting.The application of other useful protecting groups, salt forms, etc. iswithin the ability of the skilled artisan.

[0077] As defined above and as used herein, substituent groups maythemselves be substituted. Such substitution may be with one or moresubstituents. Such substituents include those listed in C. Hansch and A.Leo, Substituent Constants for Correlation Analysis in Chemistry andBiology (1979), incorporated by reference herein. Preferred substituentsinclude (for example) alkyl, alkenyl, alkoxy, hydroxy, oxo, nitro,amino, aminoalkyl (e.g., aminomethyl, etc.), cyano, halo, carboxy,alkoxyaceyl (e.g., carboethoxy, etc.), thiol, aryl, cycloalkyl,heteroaryl, heterocycloalkyl (e.g., piperidinyl, morpholinyl,pyrrolidinyl, etc.), imino, thioxo, hydroxyalkyl, aryloxy, arylalkyl,and combinations thereof.

[0078] As used herein, “mammalian metalloprotease” refers to theproteases disclosed in the “Background” of this application. Prefered“mammalian metalloproteases” include any metal-containing (preferablyzinc-containing) enzyme found in animal, preferably mamalian sourcescapable of catalyzing the breakdown of collagen, gelatin or proteoglycanunder suitable assay conditions. Appropriate assay conditions can befound, for example, in U.S. Pat. No. 4,743,587, which references theprocedure of Cawston, et al., Anal. Biochem. (1979) 99:340-345, use of asynthetic substrate is described by Weingarten, H., et al., Biochem.Biophy. Res. Comm. (1984) 139:1184-1187. Any standard method foranalyzing the breakdown of these structural proteins can, of course, beused. More prefered metalloprotease enzymes are zinc-containingproteases which are similar in structure to, for example, humanstromelysin or skin fibroblast collagenase. The ability of candidatecompounds to inhibit metalloprotease activity can, of course, be testedin the assays described above. Isolated metalloprotease enzymes can beused to confirm the inhibiting activity of the invention compounds, orcrude extracts which contain the range of enzymes capable of tissuebreakdown can be used.

[0079] Compounds:

[0080] Compounds of the invention are described in the Summary of theInvention, more preferred compounds of Formula (I) include,

[0081] wherein

[0082] Ar is aryl or heteroaryl, substituted or unsubstituted;

[0083] R₁ is OH, alkoxy, NHOR₂, where R₂ is hydrogen or alkyl;

[0084] W is one or more of hydrogen, lower alkyl;

[0085] Y is independently one or more of hydroxy, SR₃, SOR₄, SO₂R₅,alkoxy, amino, wherein amino is of formula NR₆,R₇, wherein R₆ and R₇ areindependently chosen from hydrogen, alkyl, heteroalkyl, heteroaryl,aryl, OR₃, SO₂R₈, COR₉, CSR₁₀, PO(R₁₁)₂; and

[0086] R₃ is hydrogen, alkyl, aryl, heteroaryl;

[0087] R₄ is alkyl, aryl, heteroaryl;

[0088] R₈ is alkyl, aryl, heteroaryl, heteroalkyl, amino, alkylamino,dialkylamino, arylamino, diarylamino and alkylarylamino;

[0089] R₉ is hydrogen, alkoxy, aryloxy, heteroaryloxy, alkyl, aryl,heteroaryl, heteroalkyl, amino, alkylamino, dialkylamino, arylamino andalkylarylamino;

[0090] R₁₀ is alkyl, aryl, heteroaryl, heteroalkyl, amino, alkylamino,dialkylamino, arylamino, diarylamino and alkylarylamino;

[0091] R₁₁ is alkyl, aryl, heteroaryl, heteroalkyl;

[0092] Z is hydrogen;

[0093] n is 1-3.

[0094] There may be one or more W, Y and Z moieties on the molecule ofthe invention. Preferably there are five or less substituents chosenfrom W, Y and Z which are not hydrogen. Y and Z moieties may appear onthe same carbon, i.e., geminal in relation to each other.

[0095] Where Z is heteroalkylene, it is prefered that heteroatomsadjacent to the parent ring structure, more preferably such heteroalkylshave 2 to 4 members. Preferred heteroatoms are divalent.

[0096] Preferred Ar include aryl and alkyl moieties. When Ar is aryl, itincludes heterocyclic and carbocyclic aryl, either monocyclic orpolycyclic, preferably monocyclic aryl, more preferably phenyl; When Aris alkyl, it is preferably C₁ to C₁₈ alkyl, more preferably C₂ to C₈alkyl or heteroalkyl. Ar can be substituted or unsubstituted.

[0097] W is preferably a C₁ to C₄ alkyl or C₁ to C₄ alkylene bridge.When W is an alkylene bridge, it is preferably methylene, ethylene orpropylene, more preferably methylene. When W is alkyl it is preferablymethyl or ethyl, more preferably methyl.

[0098] The variable “n” alters the size of the nitrogen containing ring,more preferred ring sizes are 5 and 6 membered rings.

[0099] Compound Preparation:

[0100] The hydroxamic compounds of Formula (I) can be prepared using avariety of procedures.

[0101] The starting materials used in preparing the compounds of theinvention are known, made by known methods, or are commerciallyavailable as a starting material. General schemes and representativeexamples of the preparation of compounds of the invention follow.

[0102] In the following scheme W and Z are omitted for clarity. Theskilled artisan will appreciate that Z may be added using similarmethodologies or those known in the art. W may be added by artrecognized methodologies as well. For compounds where Y is not adjacentto the ring nitrogen, a preferred method of making the compounds is;

[0103] Where R is a derivatizable group or can be manipulated orsubstituted, such compounds are known or are prepared by known methods.For example, when R is OH, and n is 1, hydroxyproline (A) is convertedto its analogous sultamester and the hydroxyl is then manipulated togive (B) during this or a subsequent step Y and Z can be added oraltered, followed by treatment with hydroxyl amine under basicconditions to give (C).

[0104] R′ may be a protecting group, a free acid or any moiety theskilled artisan prefers, provided that ultimately it provides thecompounds of the invention.

[0105] A variety of compounds can be generated in a similar fashion,using the guidance of the scheme above.

[0106] It is recognized that it is preferable to use a protecting groupfor any reactive functionality such as a carboxyl, hydroxyl and thelike, during the formation of the sultamester. This is standardpractice, well within the normal practice of the skilled artisan.

[0107] In the above schemes, where R is alkoxy or alkylthio, thecorresponding hydroxy or thiol compounds are derived from the finalcompounds by using a standard dealkylating procedure (Bhatt, et al.,“Cleavage of Ethers”, Synthesis, 1983, pp. 249-281).

[0108] These steps may be varied to increase yield of desired product.The skilled artisan will recognize the judicious choice of reactants,solvents, and temperatures is an important component in any successfulsynthesis. Determination of optimal conditions, etc. is routine. Thusthe skilled artisan can make a variety of compounds using the guidanceof the scheme above.

[0109] It is recognized that the skilled artisan in the art of organicchemistry can readily carry out standard manipulations of organiccompounds without further direction; that is, it is well within thescope and practice of the skilled artisan to carry out suchmanipulations. These include, but are not limited to, reduction ofcarbonyl compounds to their corresponding alcohols, oxidations ofhydroxyls and the like, acylations, aromatic substitutions, bothelectrophilic and nucleophilic, etherifications, esterification andsaponification and the like. Examples of these manipulations arediscussed in standard texts such as March, Advanced Organic Chemistry(Wiley), Carey and Sundberg, Advanced Organic Chemistry (Vol. 2) andother art that the skilled artisan is aware of.

[0110] The skilled artisan will readily appreciate that certainreactions are best carried out when other potentially reactivefunctionality on the molecule is masked or protected, thus avoiding anyundesirable side reactions and/or increasing the yield of the reaction.Often the skilled artisan utilizes protecting groups to accomplish suchincreased yields or to avoid the undesired reactions. These reactionsare found in the literature and are also well within the scope of theskilled artisan. Examples of many of these manipulations can be foundfor example in T. Greene, Protecting Groups in Organic Synthesis. Ofcourse, amino acids used as starting materials with reactive side chainsare preferably blocked to prevent undesired side reactions.

[0111] The compounds of the invention may have one or more chiralcenters. As a result, one may selectively prepare one optical isomer,including diastereomer and enantiomer, over another, for example bychiral starting materials, catalysts or solvents, or may prepare bothstereoisomers or both optical isomers, including diastereomers andenantiomers at once (a racemic mixture). Since the compounds of theinvention may exist as racemic mixtures, mixtures of optical isomers,including diastereomers and enantiomers, or stereoisomers may beseparated using known methods, such as chiral salts, chiralchromatography and the like.

[0112] In addition, it is recognized that one optical isomer, includingdiastereomer and enantiomer, or stereoisomer may have favorableproperties over the other. Thus when disclosing and claiming theinvention, when one racemic mixture is disclosed, it is clearlycontemplated that both optical isomers, including diastereomers andenantiomers, or stereoisomers substantially free of the other aredisclosed and claimed as well.

[0113] Methods of use

[0114] Metalloproteases (MPs) found in the body operate, in part, bybreaking down the extracellular matrix, which comprises extracellularproteins and glycoproteins. These proteins and glycoproteins play animportant role in maintaining the size, shape, structure and stabilityof tissue in the body. Inhibitors of metalloproteases are useful intreating diseases caused, at least in part, by breakdown of suchproteins. It is known that MPs are intimately involved in tissueremodeling. As a result of this activity they have been said to beactive in many disorders involving either the:

[0115] breakdown of tissues; including degenerative diseases, such asarthritis, multiple sclerosis and the like; metastasis or mobility oftissues in the body:

[0116] the remodeling of tissues, including fibrotic disease, scarring,benign hyperplasia, and the like.

[0117] The compounds of the present invention treat disorders, diseasesand/or unwanted conditions which are characterized by unwanted orelevated activity by that class of proteases. For example the compoundscan be used to inhibit proteases which

[0118] destroy structural proteins (i.e. the proteins that maintaintissue stability and structure);

[0119] interfere in inter/intracellular signaling, including thoseimplicated in cytokine up-regulation, and/or cytokine processing and/orinflammation, tissue degradation and other maladies [Mohler K M, et al,Nature 370 (1994) 218-220, Gearing A J H, et al, Nature 370 (1994)555-557 McGeehan G M, et al, Nature 370 (1994) 558-561], and/or

[0120] facilitate processes which are undesired in the subject beingtreated, for example, the processes of sperm maturation, eggfertilization and the like.

[0121] As used herein, a “MP related disorder” or “a MP related disease”is one that involves unwanted or elevated MP activity in the biologicalmanifestation of the disease or disorder; in the biological cascadeleading to the disorder; or as a symptom of the disorder. This“involvement” of the MP includes;

[0122] The unwanted or elevated MP activity as a cause of the disorderor biological manifestation, whether the activity was elevatedgenetically, by infection, by autoimmunity, trauma, biomechanicalcauses, lifestyle [e.g. obesity] or by some other cause;

[0123] The MP as part of the observable manifestation of the disease ordisorder. That is, the disease or disorder is measurable in terms of theincreased MP activity, or from a clinical standpoint, unwanted orelevated MP levels indicate the disease. MPs need not be the “hallmark”of the disease or disorder;

[0124] The unwanted or elevated MP activity is part of the biochemicalor cellular cascade that results or relates to the disease or disorder.In this respect, inhibition of the MP activity interrupts the cascade,and thus controls the disease.

[0125] Advantageously, many MPs are not distributed evenly throughoutthe body. Thus the distribution of MPs expressed in various tissues areoften specific to those tissues. For example, the distribution ofmetalloproteases implicated in the breakdown of tissues in the joints,is not the same as the distribution of metalloproteases found in othertissues. Thus, though not essential for activity or efficacy, certaindisorders preferably are treated with compounds that act on specific MPsfound in the affected tissues or regions of the body. For example, acompound which displays a higher degree of affinity and inhibition for aMP found in the joints (e.g. chondrocytes) would be preferred fortreatment of disease found there than other compounds which are lessspecific.

[0126] In addition, certain inhibitors are more bioavialable to certaintissues than others, and this judicious choice of inhibitor, with theselectivity described above provides for specific treatment of thedisorder, disease or unwanted condition. For example, compounds of thisinvention vary in their ability to penetrate into the central nervoussystem. Thus compounds may be selected to produce effects mediatedthrough MPs found specifically outside the central nervous system.

[0127] Determination of the specificity of a MP inhibitor of a certainMP is within the skill of the artisan in that field. Appropriate assayconditions can be found in the literature. Specifically assays are knownfor stromelysin and collagenase. For example, U.S. Pat. No. 4,743,587references the procedure of Cawston, et al., Anal Biochem (1979)99:340-345. The use of a synthetic substrate in an assay is described byWeingarten, H., et al., Biochem Biophy Res Comm (1984) 139:1184-1187.Any standard method for analyzing the breakdown of structural proteinsby MPs can, of course, be used. The ability of compounds of theinvention to inhibit metalloprotease activity can, of course, be testedin the assays found in the literature, or variations thereof. Isolatedmetalloprotease enzymes can be used to confirm the inhibiting activityof the invention compounds, or crude extracts which contain the range ofenzymes capable of tissue breakdown can be used.

[0128] As a result of the MP inhibitory effect of the compounds of theinvention, the compounds of the invention are also useful in treatingthe following disorders by virtue of their metalloprotease activity.

[0129] The compounds of this invention are also useful for theprophylactic or acute treatment. They are administered in any way theskilled artisan in the fields of medicine or pharmacology would desire.It is immediately apparent to the skilled artisan that preferred routesof administration will depend upon the disease state being treated, andthe dosage form chosen. Preferred routes for systemic administrationinclude administration perorally or parenterally.

[0130] However, the skilled artisan will readily appreciate theadvantage of administering the MP inhibitor directly to the affectedarea for many disorders. For example, it may be advantageous toadminister MP inhibitors directly to the area of the disease orcondition as in area affected by surgical trauma (e.g., angioplasty),area affected by scarring or burn (e.g., topical to the skin), Becausethe remodeling of bone involves MPs, the compounds of the invention areuseful in preventing prosthesis loosening. It is known in the art thatover time prostheses loosen, become painful, and may result in furtherbone injury, thus demanding replacement. The need for replacement ofsuch prostheses includes those such as in, joint replacements (forexample hip, knee and shoulder replacements), dental prosthesis,including dentures, bridges and prosthesis secured to the maxilla and/ormandible.

[0131] MPs are also active in remodeling of the cardiovascular system(for example, in congestive heart failure). It has been suggested thatone of the reasons angioplasty has a higher than expected long termfailure rate (reclosure over time) is that MP activity is not desired oris elevated in response to what may be recognized by the body as“injury” to the basement membrane of the vessel. Thus regulation of MPactivity in indications such as dilated cardiomyopathy, congestive heartfailure, atherosclerosis, plaque rupture, reperfusion injury, ischemia,chronic obstructive pulmonary disease, angioplasty restenosis and aorticaneurysm may increase long term success of any other treatment, or maybe a treatment in itself.

[0132] In skin care, MPs are implicated in the remodeling or turnover ofskin. As a result, the regulation of MPs improves treatment of skinconditions including but not limited to, wrinkle repair, regulation andprevention and repair of ultraviolet induced skin damage. Such atreatment includes prophylactic treatment or treatment before thephysiological manifestations are obvious. For example, the MP may beapplied as a pre-exposure treatment to prevent ultraviolet damage and/orduring or after exposure to prevent or minimize post-exposure damage. Inaddition, MPs are implicated in skin disorders and diseases related toabnormal tissues that result from abnormal turnover, which includesmetalloprotease activity, such as epidermolysis bullosa, psoriasis,scleroderma and atopic dermatitis. The compounds of the invention arealso useful for treating the consequences of normal injury to the skinincluding scarring or contraction of tissue, for example, followingburns. MP inhibition is also useful in surgical procedures involving theskin for prevention of scarring, and promotion of normal tissue growthincluding in such applications as limb reattachment and refractorysurgery (whether by laser or incision).

[0133] In addition, MPs are related to disorders involving irregularremodeling of other tissues, such as bone, for example, in otosclerosisand/or osteoporosis, or for specific organs, such as in liver cirrhosisand fibrotic lung disease. Similarly in diseases such as multiplesclerosis, MPs may be involved in the irregular modeling of blood brainbarrier and/or myelin sheaths of nervous tissue. Thus regulating MPactivity may be used as a strategy in treating, preventing, andcontrolling such diseases.

[0134] MPs are also thought to be involved in many infections, includingcytomegalovirus; [CMV] retinitis; HIV, and the resulting syndrome, AIDS.

[0135] MPs may also be involved in extra vascularization wheresurrounding tissue needs to be broken down to allow new blood vesselssuch as in angiofibroma and hemangioma.

[0136] Since MPs break down the extracellular matrix, it is contemplatedthat inhibitors of these enzymes can be used as birth control agents,for example in preventing ovulation, in preventing penetration of thesperm into and through the extracellular milieu of the ovum,implantation of the fertilized ovum and in preventing sperm maturation.

[0137] In addition they are also contemplated to be useful in preventingor stopping premature labor and delivery.

[0138] Since MPs are implicated in the inflammatory response, and in theprocessing of cytokines the compounds are also useful asanti-inflammatories, for use in disease where inflammation is prevalentincluding, inflammatory bowel disease, Crohn's disease, ulcerativecolitis, pancreatitis, diverticulitis, asthma or related lung disease,rheumatoid arthritis, gout and Reiter's Syndrome.

[0139] Where autoimmunity is the cause of the disorder, the immuneresponse often triggers MP and cytokine activity. Regulation of MPs intreating such autoimmune disorders is a useful treatment strategy. ThusMP inhibitors can be used for treating disorders including, lupuserythmatosis, ankylosing spondylitis, and autoimmune keratitis.Sometimes the side effects of autoimmune therapy result in exacerbationof other conditions mediated by MPs, here MP inhibitor therapy iseffective as well, for example, in autoimmune-therapy-induced fibrosis.

[0140] In addition, other fibrotic diseases lend themselves to this typeof therapy, including pulmonary disease, bronchitis, emphysema, cysticfibrosis, acute respiratory distress syndrome (especially the acutephase response).

[0141] Where MPs are implicated in the undesired breakdown of tissue byexogenous agents, these can be treated with MP inhibitors. For example,they are effective as rattle snake bite antidote, as anti-vessicants, intreating allergic inflammation, septicemia and shock. In addition, theyare useful as antiparasitics (e.g., in malaria) and antiinfectives. Forexample, they are thought to be useful in treating or preventing viralinfection, including infection which would result in herpes, “cold”(e.g., rhinoviral infection), meningitis, hepatitis, HIV infection andAIDS.

[0142] MP inhibitors are also thought to be useful in treatingAlzheimer's disease, amyotrophic lateral sclerosis (ALS), musculardystrophy, complications resulting from or arising out of diabetes,especially those involving loss of tissue viability, coagulation, Graftvs. Host disease, leukemia, cachexia, anorexia, proteinuria, and perhapsregulation of hair growth.

[0143] For some diseases, conditions or disorders MP inhibition iscontemplated to be a preferred method of treatment. Such diseases,conditions or disorders include, arthritis (including osteoarthritis andrheumitoid arthritis), cancer (especially the prevention or arrest oftumor growth and metastasis), ocular disorders (especially cornealulceration, lack of corneal healing, macular degeneration, andpterygium), and gum disease (especially periodontal disease, andgingivitis)

[0144] Compounds preferred for, but not limited to, the treatment ofarthritis (including osteoarthritis and rheumatoid arthritis) are thosecompounds that are selective for the matrix metalloproteases and thedisintegrin metalloproteases.

[0145] Compounds preferred for, but not limited to, the treatment ofcancer (especially the prevention or arrest of tumor growth andmetastasis) are those compounds that preferentially inhibit gelatinasesor type IV collagenases.

[0146] Compounds preferred for, but not limited to, the treatment ofocular disorders (especially corneal ulceration, lack of cornealhealing, macular degeneration, and pterygium) are those compounds thatbroadly inhibit metalloproteases. Preferably these compounds areadministered topically, more preferably as a drop or gel.

[0147] Compounds preferred for, but not limited to, the treatment of gumdisease (especially periodontal disease, and gingivitis) are thosecompounds that preferentially inhibit collagenases.

[0148] Compositions:

[0149] The compositions of the invention comprise:

[0150] (a) a safe and effective amount of a compound of Formula (I); and

[0151] (b) a pharmaceutically-acceptable carrier.

[0152] As discussed above, numerous diseases are known to be mediated byexcess or undesired metalloprotease activity. These include tumormetastasis, osteoarthritis, rheumatoid arthritis, skin inflammation,ulcerations, particularly of the cornea, reaction to infection,periodontitis and the like. Thus, the compounds of the invention areuseful in therapy with regard to conditions involving this unwantedactivity.

[0153] The invention compounds can therefore be formulated intopharmaceutical compositions for use in treatment or prophylaxis of theseconditions. Standard pharmaceutical formulation techniques are used,such as those disclosed in Remington's Pharmaceutical Sciences, MackPublishing Company, Easton, Pa., latest edition.

[0154] A “safe and effective amount” of a Formula (I) compound is anamount that is effective, to inhibit metalloproteases at the site(s) ofactivity, in an animal, preferably a mammal, more preferably a humansubject, without undue adverse side effects (such as toxicity,irritation, or allergic response), commensurate with a reasonablebenefit/risk ratio when used in the manner of this invention. Thespecific “safe and effective amount” will, obviously, vary with suchfactors as the particular condition being treated, the physicalcondition of the patient, the duration of treatment, the nature ofconcurrent therapy (if any), the specific dosage form to be used, thecarrier employed, the solubility of the Formula (I) compound therein,and the dosage regimen desired for the composition.

[0155] In addition to the subject compound, the compositions of thesubject invention contain a pharmaceutically-acceptable carrier. Theterm “pharmaceutically-acceptable carrier”, as used herein, means one ormore compatible solid or liquid filler diluents or encapsulatingsubstances which are suitable for administration to an animal,preferably a mammal, more preferably a human. The term “compatible”, asused herein, means that the components of the composition are capable ofbeing commingled with the subject compound, and with each other, in amanner such that there is no interaction which would substantiallyreduce the pharmaceutical efficacy of the composition under ordinary usesituations. Pharmaceutically-acceptable carriers must, of course, be ofsufficiently high purity and sufficiently low toxicity to render themsuitable for administration to the animal, preferably a mammal, morepreferably a human being treated.

[0156] Some examples of substances which can serve aspharmaceutically-acceptable carriers or components thereof are sugars,such as lactose, glucose and sucrose; starches, such as corn starch andpotato starch; cellulose and its derivatives, such as sodiumcarboxymethyl cellulose, ethyl cellulose, and methyl cellulose; powderedtragacanth; malt; gelatin; talc; solid lubricants, such as stearic acidand magnesium stearate; calcium sulfate; vegetable oils, such as peanutoil, cottonseed oil, sesame oil, olive oil, corn oil and oil oftheobroma; polyols such as propylene glycol, glycerine, sorbitol,mannitol, and polyethylene glycol; alginic acid; emulsifiers, such asthe Tweens″; wetting agents, such sodium lauryl sulfate; coloringagents; flavoring agents; tableting agents, stabilizers; antioxidants;preservatives; pyrogen-free water; isotonic saline; and phosphate buffersolutions.

[0157] The choice of a pharmaceutically-acceptable carrier to be used inconjunction with the subject compound is basically determined by the waythe compound is to be administered.

[0158] If the subject compound is to be injected, the preferredpharmaceutically-acceptable carrier is sterile, physiological saline,with blood-compatible suspending agent, the pH of which has beenadjusted to about 7.4.

[0159] In particular, pharmaceutically-acceptable carriers for systemicadministration include sugars, starches, cellulose and its derivatives,malt, gelatin, talc, calcium sulfate, vegetable oils, synthetic oils,polyols, alginic acid, phosphate buffer solutions, emulsifiers, isotonicsaline, and pyrogen-free water. Preferred carriers for parenteraladministration include propylene glycol, ethyl oleate, pyrrolidone,ethanol, and sesame oil. Preferably, the pharmaceutically-acceptablecarrier, in compositions for parenteral administration, comprises atleast about 90% by weight of the total composition.

[0160] The compositions of this invention are preferably provided inunit dosage form. As used herein, a “unit dosage form” is a compositionof this invention containing an amount of a Formula (I) compound that issuitable for administration to an animal, preferably a mammal, morepreferably a human subject, in a single dose, according to good medicalpractice. These compositions preferably contain from about 5 mg(milligrams) to about 1000 mg, more preferably from about 10 mg to about500 mg, more preferably from about 10 mg to about 300 mg, of a Formula(I) compound.

[0161] The compositions of this invention may be in any of a variety offorms, suitable (for example) for oral, rectal, topical, nasal, ocularor parenteral administration. Depending upon the particular route ofadministration desired, a variety of pharmaceutically-acceptablecarriers well-known in the art may be used. These include solid orliquid fillers, diluents, hydrotropes, surface-active agents, andencapsulating substances. Optional pharmaceutically-active materials maybe included, which do not substantially interfere with the inhibitoryactivity of the Formula (I) compound. The amount of carrier employed inconjunction with the Formula (I) compound is sufficient to provide apractical quantity of material for administration per unit dose of theFormula (I) compound. Techniques and compositions for making dosageforms useful in the methods of this invention are described in thefollowing references, all incorporated by reference herein: ModernPharmaceutics, Chapters 9 and 10 (Banker & Rhodes, editors, 1979);Lieberman et al., Pharmaceutical Dosage Forms: Tablets (1981); andAnsel, Introduction to Pharmaceutical Dosage Forms 2d Edition (1976).

[0162] In addition to the subject compound, the compositions of thesubject invention contain a pharmaceutically-acceptable carrier. Theterm “pharmaceutically-acceptable carrier”, as used herein, means one ormore compatible solid or liquid filler diluents or encapsulatingsubstances which are suitable for administration to an animal,preferably a mammal, more preferably a human. The term “compatible”, asused herein, means that the components of the composition are capable ofbeing commingled with the subject compound, and with each other, in amanner such that there is no interaction which would substantiallyreduce the pharmaceutical efficacy of the composition under ordinary usesituations. Pharmaceutically-acceptable carriers must, of course, be ofsufficiently high purity and sufficiently low toxicity to render themsuitable for administration to the animal, preferably a mammal, morepreferably a human being treated.

[0163] Some examples of substances which can serve aspharmaceutically-acceptable carriers or components thereof are sugars,such as lactose, glucose and sucrose; starches, such as corn starch andpotato starch; cellulose and its derivatives, such as sodiumcarboxymethyl cellulose, ethyl cellulose, and methyl cellulose; powderedtragacanth; malt; gelatin; talc; solid lubricants, such as stearic acidand magnesium stearate; calcium sulfate; vegetable oils, such as peanutoil, cottonseed oil, sesame oil, olive oil, corn oil and oil oftheobroma; polyols such as propylene glycol, glycerine, sorbitol,mannitol, and polyethylene glycol; alginic acid; emulsifiers, such asthe Tweens″; wetting agents, such sodium lauryl sulfate; coloringagents; flavoring agents; tableting agents, stabilizers; antioxidants;preservatives; pyrogen-free water; isotonic saline; and phosphate buffersolutions.

[0164] The choice of a pharmaceutically-acceptable carrier to be used inconjunction with the subject compound is basically determined by the waythe compound is to be administered.

[0165] If the subject compound is to be injected, the preferredpharmaceutically-acceptable carrier is sterile, physiological saline,with blood-compatible suspending agent, the pH of which has beenadjusted to about 7.4.

[0166] Various oral dosage forms can be used, including such solid formsas tablets, capsules, granules and bulk powders. These oral formscomprise a safe and effective amount, usually at least about 5%, andpreferably from about 25% to about 50%, of the Formula (I) compound.Tablets can be compressed, tablet triturates, enteric-coated,sugar-coated, film-coated, or multiple-compressed, containing suitablebinders, lubricants, diluents, disintegrating agents, coloring agents,flavoring agents, flow-inducing agents, and melting agents. Liquid oraldosage forms include aqueous solutions, emulsions, suspensions,solutions and/or suspensions reconstituted from non-effervescentgranules, and effervescent preparations reconstituted from effervescentgranules, containing suitable solvents, preservatives, emulsifyingagents, suspending agents, diluents, sweeteners, melting agents,coloring agents and flavoring agents.

[0167] The pharmaceutically-acceptable carrier suitable for thepreparation of unit dosage forms for peroral administration arewell-known in the art. Tablets typically comprise conventionalpharmaceutically-compatible adjuvants as inert diluents, such as calciumcarbonate, sodium carbonate, mannitol, lactose and cellulose; binderssuch as starch, gelatin and sucrose; disintegrants such as starch,alginic acid and croscarmelose; lubricants such as magnesium stearate,stearic acid and talc. Glidants such as silicon dioxide can be used toimprove flow characteristics of the powder mixture. Coloring agents,such as the FD&C dyes, can be added for appearance. Sweeteners andflavoring agents, such as aspartame, saccharin, menthol, peppermint, andfruit flavors, are useful adjuvants for chewable tablets. Capsulestypically comprise one or more solid diluents disclosed above. Theselection of carrier components depends on secondary considerations liketaste, cost, and shelf stability, which are not critical for thepurposes of the subject invention, and can be readily made by a personskilled in the art.

[0168] Peroral compositions also include liquid solutions, emulsions,suspensions, and the like. The pharmaceutically-acceptable carrierssuitable for preparation of such compositions are well known in the art.Typical components of carriers for syrups, elixirs, emulsions andsuspensions include ethanol, glycerol, propylene glycol, polyethyleneglycol, liquid sucrose, sorbitol and water. For a suspension, typicalsuspending agents include methyl cellulose, sodium carboxymethylcellulose, Avicel″ RC-591, tragacanth and sodium alginate; typicalwetting agents include lecithin and polysorbate 80; and typicalpreservatives include methyl paraben and sodium benzoate. Peroral liquidcompositions may also contain one or more components such as sweeteners,flavoring agents and colorants disclosed above.

[0169] Such compositions may also be coated by conventional methods,typically with pH or time-dependent coatings, such that the subjectcompound is released in the gastrointestinal tract in the vicinity ofthe desired topical application, or at various times to extend thedesired action. Such dosage forms typically include, but are not limitedto, one or more of cellulose acetate phthalate, polyvinylacetatephthalate, hydroxypropyl methyl cellulose phthalate, ethyl cellulose,Eudragit″ coatings, waxes and shellac.

[0170] Compositions of the subject invention may optionally includeother drug actives.

[0171] Other compositions useful for attaining systemic delivery of thesubject compounds include sublingual, buccal and nasal dosage forms.Such compositions typically comprise one or more of soluble fillersubstances such as sucrose, sorbitol and mannitol; and binders such asacacia, microcrystalline cellulose, carboxymethyl cellulose andhydroxypropyl methyl cellulose. Glidants, lubricants, sweeteners,colorants, antioxidants and flavoring agents disclosed above may also beincluded.

[0172] The compositions of this invention can also be administeredtopically to a subject, e.g., by the direct laying on or spreading ofthe composition on the epidermal or epithelial tissue of the subject, ortransdermally via a “patch”. Such compositions include, for example,lotions, creams, solutions, gels and solids. These topical compositionspreferably comprise a safe and effective amount, usually at least about0.1%, and preferably from about 1% to about 5%, of the Formula (I)compound. Suitable carriers for topical administration preferably remainin place on the skin as a continuous film, and resist being removed byperspiration or immersion in water. Generally, the carrier is organic innature and capable of having dispersed or dissolved therein the Formula(I) compound. The carrier may include pharmaceutically-acceptableemolients, emulsifiers, thickening agents, solvents and the like.

[0173] Methods of Administration:

[0174] This invention also provides methods of treating or preventingdisorders associated with excess or undesired metalloprotease activityin a human or other animal subject, by administering a safe andeffective amount of a Formula (I) compound to said subject. As usedherein, a “disorder associated with excess or undesired metalloproteaseactivity” is any disorder characterized by degradation of matrixproteins. The methods of the invention are useful in treating disordersdescribed above.

[0175] The Formula (I) compounds and compositions of this invention canbe administered topically or systemically. Systemic application includesany method of introducing Formula (I) compound into the tissues of thebody, e.g., intra-articular (especially in treatment of rheumatoidarthritis), intrathecal, epidural, intramuscular, transdermal,intravenous, intraperitoneal, subcutaneous, sublingual, rectal, and oraladministration. The Formula (I) compounds of the present invention arepreferably administered orally.

[0176] The specific dosage of inhibitor to be administered, as well asthe duration of treatment, and whether the treatment is topical orsystemic are interdependent. The dosage and treatment regimen will alsodepend upon such factors as the specific Formula (I) compound used, thetreatment indication, the ability of the Formula (I) compound to reachminimum inhibitory concentrations at the site of the metalloprotease tobe inhibited, the personal attributes of the subject (such as weight),compliance with the treatment regimen, and the presence and severity ofany side effects of the treatment.

[0177] Typically, for a human adult (weighing approximately 70kilograms), from about 5 mg to about 3000 mg, more preferably from about5 mg to about 1000 mg, more preferably from about 10 mg to about 100 mg,of Formula (I) compound are administered per day for systemicadministration. It is understood that these dosage ranges are by way ofexample only, and that daily administration can be adjusted depending onthe factors listed above.

[0178] A preferred method of administration for treatment of rheumatoidarthritis is oral or parenterally via intra-articular injection. As isknown and practiced in the art, all formulations for parenteraladministration must be sterile. For mammals, especially humans,(assuming an approximate body weight of 70 kilograms) individual dosesof from about 10 mg to about 1000 mg are preferred.

[0179] A preferred method of systemic administration is oral. Individualdoses of from about 10 mg to about 1000 mg, preferably from about 10 mgto about 300 mg are preferred.

[0180] Topical administration can be used to deliver the Formula (I)compound systemically, or to treat a subject locally. The amounts ofFormula (I) compound to be topically administered depends upon suchfactors as skin sensitivity, type and location of the tissue to betreated, the composition and carrier (if any) to be administered, theparticular Formula (I) compound to be administered, as well as theparticular disorder to be treated and the extent to which systemic (asdistinguwashed from local) effects are desired.

[0181] The inhibitors of the invention can be targeted to specificlocations where the metalloprotease is accumulated by using targetingligands. For example, to focus the inhibitors to metalloproteasecontained in a tumor, the inhibitor is conjugated to an antibody orfragment thereof which is immunoreactive with a tumor marker as isgenerally understood in the preparation of immunotoxins in general. Thetargeting ligand can also be a ligand suitable for a receptor which ispresent on the tumor. Any targeting ligand which specifically reactswith a marker for the intended target tissue can be used. Methods forcoupling the invention compound to the targeting ligand are well knownand are similar to those described below for coupling to carrier. Theconjugates are formulated and administered as described above.

[0182] For localized conditions, topical administration is preferred.For example, to treat ulcerated cornea, direct application to theaffected eye may employ a formulation as eyedrops or aerosol. Forcorneal treatment, the compounds of the invention can also be formulatedas gels, drops or ointments, or can be incorporated into collagen or ahydrophilic polymer shield. The materials can also be inserted as acontact lens or reservoir or as a subconjunctival formulation. Fortreatment of skin inflammation, the compound is applied locally andtopically, in a gel, paste, salve or ointment. The mode of treatmentthus reflects the nature of the condition and suitable formulations forany selected route are available in the art.

[0183] In all of the foregoing, of course, the compounds of theinvention can be administered alone or as mixtures, and the compositionsmay further include additional drugs or excipients as appropriate forthe indication.

[0184] Some of the compounds of the invention also inhibit bacterialmetalloproteases. Some bacterial metalloproteases may be less dependenton the stereochemistry of the inhibitor, whereas substantial differencesare found between diastereomers in their ability to inactivate themammalian proteases. Thus, this pattern of activity can be used todistinguish between the mammalian and bacterial enzymes.

[0185] Preparation and Use of Antibodies:

[0186] The invention compounds can also be utilized in immunizationprotocols to obtain antisera immunospecific for the invention compounds.As the invention compounds are relatively small, they are advantageouslycoupled to antigenically neutral carriers such as the conventionallyused keyhole limpet hemocyanin (KLH) or serum albumin carriers. Forthose invention compounds having a carboxyl functionality, coupling tocarrier can be done by methods generally known in the art. For example,the carboxyl residue can be reduced to an aldehyde and coupled tocarrier through reaction with sidechain amino groups in protein-basedcarriers, optionally followed by reduction of imino linkage formed. Thecarboxyl residue can also be reacted with sidechain amino groups usingcondensing agents such as dicyclohexyl carbodiimde or other carbodiimdedehydrating agents.

[0187] Linker compounds can also be used to effect the coupling; bothhomobifunctional and heterobifunctional linkers are available fromPierce Chemical Company, Rockford, Ill. The resulting immunogeniccomplex can then be injected into suitable mammalian subjects such asmice, rabbits, and the like. Suitable protocols involve repeatedinjection of the immunogen in the presence of adjuvants according to aschedule which boosts production of antibodies in the serum. The titersof the immune serum can readily be measured using immnunoassayprocedures, now standard in the art, employing the invention compoundsas antigens.

[0188] The antisera obtained can be used directly or monoclonalantibodies may be obtained by harvesting the peripheral bloodlymphocytes or the spleen of the immunized animal and immortalizing theantibody-producing cells, followed by identityig the suitable antibodyproducers using standard immunoassay techniques.

[0189] The polyclonal or monoclonal preparations are then useful inmonitoring therapy or prophylaxis regimens involving the compounds ofthe invention. Suitable samples such as those derived from blood, serum,urine, or saliva can be tested for the presence of the administeredinhibitor at various times during the treatment protocol using standardimmunoassay techniques which employ the antibody preparations of theinvention.

[0190] The invention compounds can also be coupled to labels such asscintigraphic labels, e.g., technetium 99 or I-131, using standardcoupling methods. The labeled compounds are administered to subjects todetermine the locations of excess amounts of one or moremetalloproteases in vivo. The ability of the inhibitors to selectivelybind metalloprotease is thus taken advantage of to map the distributionof these enzymes in situ. The techniques can also be employed inhistological procedures and the labeled invention compounds can be usedin competitive imunoassays.

[0191] The following non-limiting examples illustrate the compounds,compositions, and uses of the present invention.

EXAMPLES

[0192] Compounds are analyzed using ¹H and ¹³C NMR, Elemental analysis,mass spectra and/or IR spectra, as appropriate.

[0193] Typically tetrahydrofuran (THF) is distilled from sodium andbenzophenone, diisopropylamine is distilled from calcium hydride and allother solvents are purchased as the appropriate grade. Chromatography isperformed on silica gel (70 - 230 mesh; Aldrich) or (230-400 mesh; Merk)as appropriate. Thin layer chromatography analysis (TLC) is performed onglass mounted silica gel plates (200-300 mesh; Baker) and visualizedwith UV or 5% phosphomolybdic acid in EtOH.

Examples 1-25

[0194] The following chart shows the structure of compounds madeaccording to the description in Examples 1-19 described below:

Example *(configuration) Y Z 1 d a-OH H 2 d b-OH H 3 l a-OH H 4 l b-OH H5 d b-OMe H 6 d b-(2-benzathiazole) H 7 d a-(2-benzathiazole) H 8 db-2(3N-methyl-imidazole) H 9 d a-2(3N-methyl-imidazole) H 10 d b-OPh H11 d b-O(C₆H₄)OCH₂Ph H 12 d b-O(2-(C₆H₄)NHPh) H 13 d b-O(3-Pyridyl) H 14d b-SPh H 15 d b-S(4-C₆H₄OMe) H 16 d b-S(3-C₆H₄OMe) H 17 d a-OCH₂OCH₂CH₃H 18 d a-OCH₂OCH₂Ph H 19 d a-OCH₂OCH₂CH₃OCH₃ H 20 d b-SH H 21 racemic Hphenyl 22 d a-OH, b-Et H 23 d a-OH, b-Ph H 24 d b-O(4-C₆H₄-Octyl) H 25 da-OH gem-(CH₃)₂

Example 1

[0195] a.(1N)-4-Methoxyphenylsulfonyl-(2R)-carbomethoxy-4R-hydroxy-pyrrolidine:cis-Hydroxy-D-proline (50 g, 0.38 mole) is dissolved in water:dioxane(1:1, 300 mL) with trimethylamine (135 mL, 0.96 mole).4-Methoxyphenylsulfonyl chloride (87 g, 0.42 mole) is added along with2,6-dimethylaminopyridine (4.6 g, 0.038 mole) and the mixture is stirred14 hr. at room temperature. The mixture is then concentrated and dilutedwith EtOAc. Layers are separated and the organic layer is washed 2× with1N HCl, 1× with brine, dried over MgSO₄, filtered and evaporated to give83 g of solid material which is dissolved in MeOH (500 mL). Thionylchloride (50 mL) is added dropwise and the resulting mixture stirred for14 hr. The mixture is then evaporated to dryness and triturated withCHCl₃ to give a white solid which is sufficiently pure to carry forwardwithout purification. CI⁺ MS: m/z (rel intensity) 316 (M⁻+H, 100), 256(30), 146 (45).

[0196] b.(1N)-4-Methoxyphenylsulfonyl-(2R)-N-hydroxycarboxamido-(4S)-hydroxypyrrolidine:The starting methylester 1a (361 mg, 1.15 mmole) is taken in 1 mL ofmethanol, treated with NH₂OK (1.45 mL, 0.86 M in methanol, solutionprepared as described in Fieser and Fieser, Vol 1, p 478) and stirredovernight. The following morning the material is concentrated andpartitioned between EtOAc and 1N HCl. The organic layer is washed withbrine, dried over MgSO₄, filtered and evaporated to give crude materialwhich is recrystallized from hex:EtOAc at −4° C. to give the desiredwhite solid and recovered oil. ESI MS: m/z (rel intensity) 317 (M+H⁺,100), 334 (M+NH₄+, 20), 339 (M+Na⁺, 35).

Example 2

[0197] a.(1N)-4-Methoxyphenylsulfonyl-(2R)-carbomethoxy-(4S)-benzoyloxypyrrolidine:The alcohol 1a (780 mg, 2.48 mmole) is dissolved in 5 mL of methylenechloride. Benzoic acid (604 mg, 4.95 mmole) and triphenyl phosphine (779mg, 2.98 mmole) are then added, followed by diethyl azodicarboxylate(429 mL, 2.73 mmole). After 3 hrs, the reaction mixture is filtered andsilica gel is added to the filtrate to adsorb the solutes and themixture is concentrated to dryness. The resulting solid mixture ispoured onto the top of a flash silica column which is eluted with hex:EtOAc (1:1 to 0: 1) to give the desired product as a white solid. CI⁺MS: m/z (rel intensity) 420.0 (M⁺+H, 100), 250.1 (95), 126.0 (45).

[0198] b.(1N)-4-Methoxyphenylsulfonyl-(2R)-N-hydroxycarboxamido-(4S)-hydroxypyrrolidine:The methyl-benzyl diester 2a (175 g, 0.418 mmole) is taken in 2.5 mL ofmethanol, treated with NH₂OK (0.48 mL, 0.86 M in methanol, solutionprepared as described in Fieser and Fieser, Vol 1, p 478) and stirredovernight. The following morning, dry silica (1 mL) is added to themixture and the solvent removed under vaccuum. The dry silica is pouredon the top of a flash silica gel column which is subsequently elutedwith EtOAc:MeOH:HCO₂H (90:9:1) to give a white solid which is thenrecrystallized from hexane:EtOAc (1:5) to give white crystals. ESI MS:m/z (rel intensity) 317.1 (M⁻+H, 100), 339.1 (M⁺+Na, 20).

Example 3

[0199] a.(1N)-4-Methoxyphenylsulfonyl-(2S)-carbomethoxy-(4R)-hydroxypyrrolidine:To a solution of trans-4-hydroxy-L-proline methyl ester (2.0 g, 11.0mmol) in 10 mL DMF is added 2 mL N-methylmorpholine and4-methoxybenzenesulfonyl chloride and is stirred for 1 hr. The solutionis then partitioned between EtOAc and water, washed with 1 N HCl,NaHCO₃, NaCl, and dried over MgSO₄. The crude product is thenchromatographed over silica with EtOAc to give the title compound. CI⁺MS: m/z (rel intensity) 316 (100, M⁺+H).

[0200] b.(1N)-4-Methoxyphenylsulfonyl-(2S)-N-hydroxycarboxamido-(4R)-hydroxypyrrolidine:The starting ester 3a (500 mg, 1.6 mmol) is added to NH₂OK (1.9 mL, 1 eqin MeOH, prepared according to Fieser and Fieser, Vol 1, p 478) andstirred for 15 hr. The solvent is evaporated and the residue isdissolved in 1N HCl and extracted with EtOAc. The organic layer is driedover MgSO₄, evaporated and the residue is recrystallized fromEtOAc:Hexanes to give the title compound. ESI MS: m/z (rel intensity)317 (100, M⁺+H), 256 (70).

Example 4

[0201] a.(1N)-4-Methoxyphenylsulfonyl-(2S)-carbomethoxy-(4S)-hydroxy-pyrrolidine:To a solution of cis-4-hydroxy-L-proline methyl ester (2.0 g, 11.0 mmol)in 10 mL DMF is added 2 mL N-methylmorpholine and4-methoxybenzenesulfonyl chloride and is stirred for 1 hr. The solutionis then partitioned between EtOAc and water, washed with 1 N HCl,NaHCO₃, NaCl, and dried over MgSO₄. The crude product is thenchromatographed over silica with EtOAc to give the title compound. CI⁺MS: m/z (rel intensity) 316 (100, M⁺+H).

[0202] b.(1N)-4-Methoxyphenylsulfonyl-(2S)-N-hydroxycarboxamido-(4S)-hydroxy-pyrrolidine:The starting ester 4a (500 mg, 1.6 mmol) is added to NH₂OK (1.9 mL, 1 eqin MeOH, prepared according to Fieser and Fieser, Vol 1, p 478) andstirred for 15 hr. The solvent is evaporated and the residue isdissolved in 1N HCl and extracted with EtOAc. The organic layer is driedover MgSO₄, evaporated and the residue is recrystallized fromEtOAc:Hexanes to give the title compound. ESI MS: m/z (rel intensity)317 (100, M⁺+H), 256 (70).

Example 5

[0203] a.(1N)-4-Methoxyphenylsulfonyl-(2R)-carboxy-(4S)-hydroxy-pyrrolidine: Thediester 2a (10 g, 24 mmole) is dissolved in water:dioxane (1:10, 50 mL)and stirred overnight in the presence of lithium hydroxide monohydrate(5 g, 120 mmole). The mixture is acidified with 1N HCl and extractedwith EtOAc, washed with brine, dried over MgSO₄, filtered and evaporatedto give solid material which is recrystallized from EtoAc:hexanes togive the title compound as a white solid. ESI MS: m/z (rel intensity)302 (M⁺+H, 100), 318 (M⁺+NH₃, 30).

[0204] b.(1N)-4-Methoxyphenylsulfonyl-(2R)-carbomethoxy-(4S)-methoxy-pyrrolidine:The carboxylic acid 5a (4.0 g, 13.2 mmol) is stirred in THF at roomtemperature and then sodium hydride (1.58 g, 39.6 mmol, 3 equiv, 60% inoil) is slowly added. After hydrogen gas evolution had ceased, methyliodide (5.52 g, 39.6 mmol, 3 equiv) is added to the reaction mixture.The resulting solution is stirred at room temperature for 1 hour. Thereaction mixture is quenched by the addition of water and then extractedwith EtOAc. The organic extracts are concentrated to an oil and thenmethanol and 3 drops of conc. HCl are added. The solution is then heatedto reflux for 24 hours. The solvent is removed and the product ispurified by silica gel chromatography (1/1 hexane/EtOAc followed by 100%EtOAc) to afford the desired methyl ester as a white crystalline solid.CI⁺ MS: m/z (rel intensity) 330 (M+, 100).

[0205] c.(1N)-4-Methoxyphenylsulfonyl-(2R)-N-hydroxycarboxamido-(4S)-methoxypyrrolidine:The ester 5b (0.50 g, 1.52 mmol) is taken in 2 mL of methanol, treatedwith NH₂OK (2.5 mL, 0.86 M in methanol, solution prepared as describedin Fieser and Fieser, Vol 1, p 478) and stirred overnight. The solutionis poured into water and acidified to pH ˜2. The resulting solution isextracted with CH₂Cl₂, dried (Na₂SO₄) and concentrated to a white solid.Purification of the resulting solid is accomplished by recrystallizationfrom EtOAc:hexane (3:1) to afford the desired product as a whitecrystalline solid. ESI MS: m/z (rel intensity) 331.0 (M+H⁺, 100), 348.0(M+NH₄ ⁺, 85), 353.0 (M+Na⁺, 45).

Example 6

[0206] a.(1N)-4-Methoxyphenylsulfonamido-(2R)-carbomethoxy-(4R)-trifluoromethanesulfonyl-pyrrolidine:The starting alcohol 1a (221 mg, 0.702 mmole) is taken in dry CH₂Cl₂under argon and cooled to 0° C. 2,6-Lutidine (326 mL, 2.81 mmole) isadded via syringe followed by slow syringe addition oftrifluoromethanesulfonyl anhydride (153 mL, 0.912 mmole) and theresulting yellow mixture is 1 hr at 0° C. and then partitioned betweenwater and EtOAc. The organic layer is dried over MgSO₄, filtered andevaporated. The crude residue is chromatogaphed over flash silica withhexane: EtOAc (4:1 to 1:1) to give the desired off-white solid. CI⁺ MS:m/z (rel intensity) 411 (M+NH₄ ⁺, 25) 394 (M⁺+H, 21), 224 (82), 155(23), 128 (100).

[0207] b.(1N)-4-Methoxyphenylsulfonyl-(2R)-carbomethoxy-(4S)-(2-mercapto-benzothiazolyl)-pyrrolidine:The triflate 6a (145 mg, 0.353 mmole) is dissolved in methylene chloride(1 mL) under argon and 2,6-lutidine (61 mL, 0.529 mmole) is added viasyringe followed by 2-mercapto-benzothiazole (65 mg, 0.388 mmole). After1 hr., silica gel (1.5 mL) is added to the mixture which is thenevaporated to dryness. The resulting solid mixture is then added to thetop of a flash silica column which is then eluted with hexane:EtOAc (1:1to 1:5) to give the pure title compound as a clear oil. CI⁺ MS: m/z (relintensity) 465 (M⁺+H, 10), 300 (38), 240 (13), 168 (21), 150 (33), 136(100).

[0208] c.(1N)-4-Methoxyphenylsulfonyl-(2R)-N-hydroxycarboxamido-(4S)-(2-mercaptobenzothiazolyl)-pyrrolidine:A 1.76M solution of potassium hydroxyl amine in methanol is prepared.The 1.76M solution (0.4 mL, 0.711 mmoles) is added directly to themethyl ester 6b (0.165 g, 0.356 mmoles) and the reaction mixture stirredovernight. The solution is acidified with 1N HCl, then extracted 3 timeswith ethyl acetate, dried with magnesium sulfate, filtered andevaporated. Chromatography is performed on silica gel using ethylacetate:hexane:formic acid (1:1:0.1) to give the title compound. ESI MS:m/z (rel intensity) 466.0 (M⁺+H, 100), 408.2 (M⁺−Na, 20).

Example 7

[0209] a.(1N)-4-Methoxyphenylsulfonyl-(2R)-carbomethoxy-(4S)-hydroxy-pyrrolidine:The acid 5a (4 g, 9.55 mmole) is dissolved in methanol (50 mL), treatedwith thionyl chloride (3 mL) and stirred overnight. The mixture is thenevaporated to dryness and recrystallized from EtOAc:hexanes to give thetitle compound as a white solid. CI⁺ MS: m/z (rel intensity) 316 (M⁺+H,100), 256 (60), 158 (25), 146 (30).

[0210] b.(1N)-4-Methoxyphenylsulfonyl-(2R)-N-hydroxycarboxamido-(4R)-(2-mercaptobenzothiazolyl)-pyrrolidine:The starting alcohol 7a (323 mg, 1.03 mmole) is taken in 4 mL of CH₂Cl₂and to this mixture is added triphenylphosphene (351 mg, 1.35 mmole),2-mercaptobenzothiazole (189 mg, 1.13 mg), anddiethyl-diazadicarboxylate (195 mM, 1.24 mmole) and the mixture isstirred for 0.5 hr. at which time 5 mL of silica gel is added to themixture which is then concentrated to dryness. The dry residue is pouredonto the top of a flash silica column and eluted with hexane:EtOAc (4:1to 1:4) to give a clear oil. CI⁺ MS: m/z (rel intensity) 465 (M⁺+H, 5),300 (20), 150 (25), 136 (100), 128 (25).

[0211] c.(1N)-4-Methoxyphenylsulfonyl-(2R)-N-hydroxycarboxamido-(4R)-(2-mercaptobenzothiazolyl)-pyrrolidine:The methyl ester 7b (372 g, 0.802 mmole) is taken in 1.5 mL of methanol,treated with NH₂OK (1.4 mL, 0.86 M in methanol, solution prepared asdescribed in Fieser and Fieser, Vol 1, p 478) and stirred overnight. Thefollowing morning, dry silica (1.5 mL) is added to the mixture and thesolvent removed under vaccuum. The dry silica is poured on the top of aflash silica gel column which is subsequently eluted with hexane:EtOAc(1:2) to remove impurities and then EtOAC:MeOH (9:1). The resultingproduct is recrystallized from chloroform to give white crystals. ESIMS: m/z (rel intensity) 466.1 (M⁺+H, 100), 488.0 (M⁺Na, 12).

Example 8

[0212] a.(1N)-4-Methoxyphenylsulfonyl-(2R)-carbomethoxy-(4S)-[(1N)-methyl-2-mercaptoimidazyl]-pyrrolidine:The alcohol 1a (700 mg, 2.22 mmole) is dissolved in 12 mL of methylenechloride. 2-Mercapto-1-methylimidazole (304 mg, 2.66 mmole) andtriphenyl phosphine (873 mg, 3.33 mmole) are then added, followed bydiethyl azodicarboxylate (420 mL, 2.66 mmole). After 3 hrs, the reactionmixture is filtered and silica gel is added to the filtrate to adsorbthe solutes and the mixture is concentrated to dryness. The resultingsolid mixture is poured onto the top of a flash silica column which iseluted with hex:EtOAc (1:1 to 0:1) to give the desired product as awhite solid. CI⁺ MS: m/z (rel intensity) 412 (M⁺+H, 100), 242 (5), 115(28).

[0213] b.(1N)-4-Methoxyphenylsulfonyl-(2R)-N-hydroxycarboxamido-(4S)-[(1N)-methyl-2-mercaptoimidazyl]-pyrrolidine:The ester 8a (500 mg, 1.22 mmole) is taken in 1 mL of methanol, treatedwith NH₂OK (2.11 mL, 0.86 M in methanol, solution prepared as describedin Fieser and Fieser, Vol 1, p 478) and stirred overnight. The followingmorning, dry silica (2 mL) is added to the mixture and the solventremoved under vaccuum. The dry silica is poured on the top of a flashsilica gel column which is subsequently eluted with hexane:EtOAc (1:1 to0:1) followed by EtOAc:MeOH:NH₄OH (9:1:0.1) to give a white solid. ESIMS: m/z (rel intensity) 413 (M⁺+H, 100), 435 (M⁺+Na, 20).

Example 9

[0214] a.(1N)-4-Methoxyphenylsulfonyl-(2R)-carbomethoxy-(4R)-[(1N)-methyl-2-mercaptoimidazyl]-pyrrolidine:The alcohol 7a (700 mg, 2.22 mmole) is dissolved in 12 mL of methylenechloride. 2-Mercapto-1-methylimidazole (304 mg, 2.66 mmole) andtriphenyl phosphine (873 mg, 3.33 mmole) are then added, followed bydiethyl azodicarboxylate (420 mL, 2.66 mmole). After 3 hrs, the reactionmixture is filtered and silica gel is added to the filtrate to adsorbthe solutes and the mixture is concentrated to dryness. The resultingsolid mixture is poured onto the top of a flash silica column which iseluted with hex: EtOAc (1:1 to 0:1) to give the desired product as awhite solid. CI⁺ MS: m/z (rel intensity) 412 (M⁺+H, 100), 242 (5), 115(28).

[0215] b.(1N)-4-Methoxyphenylsulfonyl-(2R)-N-hydroxycarboxamido-(4R)-[(1N)-methyl-2-mercaptoimidazyl]-pyrrolidine:The ester 9a (500 mg, 1.22 mmole) is taken in 5 mL of methanol, treatedwith NH₂OK (2.11 mL, 0.86 M in methanol, solution prepared as describedin Fieser and Fieser, Vol 1, p 478) and stirred overnight. The followingmorning, dry silica (2 mL) is added to the mixture and the solventremoved under vaccuum. The dry silica is poured on the top of a flashsilica gel column which is subsequently eluted with hexane:EtOAc (1:1 to0:1) to give a white solid. ESI MS: m/z (rel intensity) 413.0 (M⁺+H,100), 435.0 (M⁺+Na, 20).

Example 10

[0216] a.(1N)-4-Methoxyphenylsulfonyl-(2R)-carbomethoxy-(4S)-phenoxy-pyrrolidine:The alcohol 1a (1.3 g, 4.12 mmole) is dissolved in 3 mL of methylenechloride. Phenol (0.8 g, 8.24 mmole) and triphenyl phosphine (2.16 g,8.24 mmole) are then added, followed by diethyl azodicarboxylate (1.2mL, 7.84 mmole). After 3 hrs, the reaction mixture is filtered andconcentrated to an oil, which is purified on silica gel using ethylacetate:hexane:methylene chloride (1:3:1) to give the desired product asan oil. CI⁺ MS: m/z (rel intensity) 409 (100, M⁻+NH₃), 392 (72, M⁺+H).

[0217] b.(1N)-4-Methoxyphenylsulfonyl-(2R)-N-hydroxycarboxamido-(4S)-phenoxypyrrolidine:The methyl ester 10a (0.6 g, 1.53 mmole) is taken in 3 mL of methanol,treated with NH₂OK (5 mL, 1.7 M in methanol, solution prepared asdescribed in Fieser and Fieser, Vol 1, p 478) and stirred overnight. Thefollowing morning, dry silica (1.5 mL) is added to the mixture and thesolvent removed under vaccuum. The dry silica is poured on the top of aflash silica gel column which is subsequently eluted with formicacid:EtOAc (0:1 to 3:97) to give 0.36 g of white foamy solid, which isrecrystallized from hexane:EtOAc to give the desired product. ESI MS:m/z (rel intensity) 415 (38, M⁺+Na), 410 (10, M⁺+NH₄), 393 (100, M⁺+H).

Example 11

[0218] a.(1N)-4-Methoxyphenylsulfonyl-(2R)-carbomethoxy-(4S)-[(4-benzyloxy)-phenoxy]-pyrrolidine:Triphenylphosphine (2.5 g, 9.51 mmole) is dissolved in 20 mL of THF.Diethyl azodicarboxylate (1.9 mL, 9.51 mmole) is added dropwise at 0° C.After 30 min with stirring, a solution of 4-(benzyloxy)phenol (2.38 g,11.9 mmole) and the alcohol 1a (1.5 g, 4.76 mmole) in 15 mL of THF isadded dropwise. The reaction is stirred at 0° C. for 30 min., roomtemperature overnight and concentrated to an oil. The crude product ispurified by flash chromatography (hexane/EtOAc, 4:1 to 1:1) on silicagel to give the desired product. CI⁺ MS: m/z (rel intensity) 498 (100,M⁺+H), 328 (24).

[0219] b.(1N)-4-Methoxyphenylsulfonyl-(2R)-N-hydroxycarboxamido-(4S)-4-benzyloxy)-phenoxypyrrolidine:The methyl ester 11a (0.7 g, 1.4 mmole) is taken in 1 mL of methanol,treated with NH₂OK (8 ml, 1.7 M in methanol, solution prepared asdescribed in Fieser and Fieser, Vol 1, p 478) and stirred for 3 hr.Silica (1.5 mL) is added to the mixture and the solvent is removed undervaccuum. The dry silica is poured on the top of a flash silica gelcolumn which is subsequently eluted with hexane:EtOAc (1:1) toEtOAc:CH₃OH (1:0 to 1:1) to give the desired product as a white foamysolid. ESI MS: m/z (rel intensity) 521 (30, M⁺+Na), 516 (14, M⁺+NH₄),499 (100, M⁺+H).

Example 12

[0220] a.(1N)-4-Methoxyphenylsulfonyl-(2R)-carbomethoxy-(4S)-(3-N-phenyl-amino)-phenoxylpyrrolidine:Triphenylphosphine (2.5 g, 9.52 mmole) is dissolved in 20 mL of THF.Diethyl azodicarboxylate (1.95 mL. 9.52 mmole) is added dropwise at 0°C. After 30 min with stirring, a solution of 3-hydroxydiphenylamine (2.2g, 11.9 mmole) and the alcohol 1a (1.5 g, 4.76 mmole) in 15 mL of THF isadded dropwise. The reaction is stirred at 0° C. for 30 min., roomtemperature for 2 hr and concentrated to an oil. The crude product ispurified by flash chromatography (hexane/EtOAc, 7:3 to 1:1) on silicagel to give the desired product. ESI MS: m/z (rel intensity) 505 (8,M⁺+Na), 483 (100, M⁺+H).

[0221] b.(1N)-4-Methoxyphenylsulfonyl-(2R)-N-hydroxycarboxamido-(4S)-3-N-phenylamino)-phenoxylpyrrolidine:The methyl ester 12a (0.68 g, 1.38 mmole) is taken in 2 mL of methanol,treated with NH₂OK (6 ml, 1.7 M in methanol, solution prepared asdescribed in Fieser and Fieser, Vol 1, p 478) and stirred overnight. Thefollowing morning, dry silica (1.5 mL) is added to the mixture and thesolvent is removed under vaccuum. The dry silica is poured on the top ofa flash silica gel column which is subsequently eluted with EtOAc:CH₃OH(1:0 to 9:1) to give the desired product as a white foamy solid. ESI MS:m/z (rel intensity) 506 (36, M⁺+Na), 484 (100, M⁺+H).

Example 13

[0222] a.(1N)-4-Methoxyphenylsulfonyl-(2R)-carbomethoxy-(4S)-(3-pyridinoxy)-pyrrolidine:Triphenylphosphine (2.42 g, 9.2 mmole) is dissolved in 20 mL of THF.Diethyl azodicarboxylate (1.81 mL, 9.2 mmole) is added dropwise at 0° C.After 30 min with stirring, a solution of 3-hydroxypyridine (1.32 g,13.83 mmole) and the alcohol 1a (1.5 g, 4.61 mmole) in 15 mL of THF isadded dropwise. The reaction is stirred at 0° C. for 30 min., roomtemperature for 2 hr and concentrated to an oil. The crude product ispurified by flash chromatography (hexane/EtOAc: 1/1 to EtOAc) on silicagel to give the desired product. CI⁺ MS: m/z (rel intensity) 393 (100,M⁻+H), 279 (88), 223 (70).

[0223] b.(1N)-4-Methoxyphenylsulfonyl-(2R)-N-hydroxycarboxamido-4S)-phenoxypyrrolidine:The methyl ester 13a (0.18 g, 0.46 mmole) is taken in 1 mL of methanol,treated with NH₂OK (0.5 ml, 1.7 M in methanol, solution prepared asdescribed in Fieser and Fieser, Vol 1, p 478) and stirred overnight. Thefollowing morning, dry silica (1.5 mL) is added to the mixture and thesolvent is removed under vaccuum. The dry silica is poured on the top ofa flash silica gel column which is subsequently eluted with EtOAc:CH₃OH(1:0 to 1:1) to give a white foamy solid which is crystallized frommethylene chloride to give the desired product as a white solid. ESI MS:m/z (rel intensity) 432 (10, M⁺+K), 416 (8, M⁺+Na), 394 (100, M⁺+H).

Example 14

[0224] a.(1N)-4-Methoxyphenylsulfonyl-(2R)-carbomethoxy-(4S)-mercaptophenylpyrrolidine:The alcohol 1a (200 mg, 0.634 mmole) is dissolved in 2 mL of methylenechloride. Thiophenol (78 mL, 0.671 mmole) and triphenyl phosphine (250mg, 0.951 mmole) are then added, followed by diethyl azodicarboxylate(120 mL, 0.761 mmole). After 3 hrs, the reaction mixture is filtered andsilica gel is added to the filtrate to adsorb the solutes and themixture is concentrated to dryness. The resulting solid mixture ispoured onto the top of a flash silica column which is eluted with hex:EtOAc (1:1 to 0:1) to give the desired white solid. CI⁺ MS: m/z (relintensity) 408 (M⁺+H, 15), 238 (100), 128 (99), 109 (93).

[0225] b.(1N)-4-Methoxyphenylsulfonamido-(2R)-N-hydroxycarboxamido-(4S)-mercaptophenylpyrrolidine:The starting methylester 14a (169 mg, 0.415 mmole) is taken in 1 mL ofmethanol, treated with NH₂OK (0.725 mL, 0.86 M in methanol, solutionprepared as described in Fieser and Fieser, Vol 1, p 478) and stirredovernight. The following morning, dry silica (1.5 mL) is added to themixture and the solvent removed under vaccuum. The dry silica is pouredon the top of a flash silica gel column which is subsequently elutedwith hexane:EtOAc (1:1 to 0:1) and then with EtOAc:MeOH:NH₄OH (9:1:0.1)to give a white solid. ESI MS: m/z (rel intensity) 409.2 (M⁺+H, 100),426.2 (M⁺+NH₄, 12), 431.1 (M⁺+Na, 25).

Example 15

[0226] a.(1N)-4-Methoxyphenylsulfonamido-(2R)-carbomethoxy-(4R)-methane-sulfonyl-pyrrolidine:The starting alcohol 1a (17.9 g, 57 mmole) is taken in dry CH₂Cl₂ (100mL) in the presence of Et₃N (25 mL) at room temperature. Methanesulfonylchloride (4.87 mL, 63 mmole) is added dropwise and the resulting mixtureis stirred overnight and the following morning the mixture is partionedbetween water and EtOAc. The organic layer is washed with brine, driedover MgSO₄, filtered and evaporated. The resulting solid isrecrystallized from EtOAc:hexanes to give the title compound as whiteprisms. CI⁺ MS: m/z (rel intensity) 411 (M+NH₄, 25) 394 (M⁺+H, 21), 224(82), 155 (23), 128 (100).

[0227] b.(1N)-4-Methoxyphenylsulfonyl-(2R)-carbomethoxy-4S)-(4-methoxy-mercaptophenyl)-pyrrolidine:The starting mesylate 15a (267 mg, 0.68 mmole) and 4-methoxythiophenol(88 mL, 0.713 mmole) are taken in THF (4 mL) at room temperature underargon and ^(t)butoxide (78 mg, 0.713 mmole) is added. The mixture isstirred for 1 hr and then partitioned between EtOAc and 1N HCl. Theorganic layer is washed with brine, dried over MgSO₄, filtered andevaporated to give 354 mg of residue which is then chromatographed overflash silica with hexane:EtOAc (8:1 to 2:1) to give the title compoundas a clear oil. CI⁺ MS: m/z (rel intensity) 438 (M⁺+H, 50), 268 (100),208 (21), 155 (81), 128 (79), 109 (45).

[0228] c.(1N)-4-Methoxyphenylsulfonyl-(2R)-N-hydroxycarboxamido-(4S)-4-methoxyphenylthioloxy)-pyrrolidine:The starting methylester 15b (129 mg, 0.295 mmole) is taken in 1 mL ofmethanol, treated with NH₂OK (0.85 mL, 0.86 M in methanol, solutionprepared as described in Fieser and Fieser, Vol 1, p 478) and stirredovernight. The following morning, dry silica (1.5 mL) is added to themixture and the solvent removed under vaccuum. The dry silica is pouredon the top of a flash silica gel column which is subsequently elutedwith hexane:EtOAc (1:2 to 0:1) to give a clear glass which is puffedinto a foamy solid by slight heating under vacuum. ESI MS: m/z (relintensity) 439 (M⁺+H, 100), 456 (M⁺+NH₃, 30).

Example 16

[0229] a.(1N)-4-Methoxyphenylsulfonyl-(2R)-carbomethoxy-(4S)-(3-methoxymercaptophenyl)-pyrrolidine:The starting mesylate 15a (267 mg, 0.68 mmole) and 3-methoxythiophenol(88 mL, 0.713 mmole) are taken in THF (4 mL) at room temperature underargon and ^(t)butoxide (78 mg, 0.713 mmole) is added. The mixture isstirred for 1 hr and then partitioned between EtOAc and 1N HCl. Theorganic layer is washed with brine, dried over MgSO₄, filtered andevaporated to give a residue which is then chromatographed over flashsilica with hexane:EtOAc (8:1 to 2:1) to give the title compound as aclear oil. CI⁺ MS: m/z (rel intensity) 438.0 (M+H⁺, 17), 268.0 (100),155 (65).

[0230] b.(1N)-4-Methoxyphenylsulfonyl-(2R)-N-hydroxycarboxamido-(4S)-(3-methoxymercaptophenyl)-pyrrolidine:The starting methylester 16a (1.58 mg, 0.361 mmole) is taken in 5 mL ofmethanol, treated with NH₂OK (0.624 mL, 0.86 M in methanol, solutionprepared as described in Fieser and Fieser, Vol 1, p 478) and stirredovernight. The following morning, dry silica (2 mL) is added to themixture and the solvent removed under vaccuum. The dry silica is pouredon the top of a flash silica gel column which is subsequently elutedwith hexane:EtOAc (1:1 to 0:1) then with EtOAc:MeOH:NH₄OH (9:1:0.1) togive a white solid. ESI MS: m/z (rel intensity) 439 (M⁺+H, 10), 456.0(M⁺+NH₄ ⁺, 40), 461.0 (M⁺+Na⁺, 27).

Example 17

[0231] a.(1N)-4-Methoxyphenylsulfonyl-(2R)-carbomethoxy-(4R)-ethyloxymethoxy-pyrrolidine:Chloroethylmethylether (0.884 mL, 9.54 mmole) was added dropwise to astirred solution of the methylester 1a (1.00 g, 3.18 mmole) in CH₂Cl₂(12 mL) and DIPEA (0.830 mL) and stirred for 16 hrs. Additional CH₂Cl₂was added and the mixture was washed with saturated NaHSO₄, dried oversodium sulfate and the solvent removed under vacuum. The dried materialwas purified over a silica column eluting first with hexane:EtOAc (8:2),followed with hexane:EtOAc (1:1) then with EtOAc to give a colorlessoil. ESI MS: m/z (rel intensity) 374.02 (M⁺+H, 100), 391.03 (M⁺+NH₃,70).

[0232] b.(1N)-4-Methoxyphenylsulfonyl-(2R)-carbomethoy-(4R)-ethyloxymethoxy-pyrrolidine:The starting methyl ester 17a (1.13 g, 3.03 mmole) is taken in 4 mL ofmethanol:tetrahydrofuran (1:1), and treated with NH₂OK (4 mL, 1.25M inmethanol) and stirred overnight. The following morning, dry silica (2.5mL) is added to the mixture and the solvent removed under vacuum. Thedry silica is poured on top of a flash silica column which issubsequently eluted with ethyl acetate followed with ethylacetate:methanol (8:2) to give a clear glass which is puffed to a foamysolid by slight heating under vacuum. The product was recrystallizedfrom cold EtOAc:hexane to give white powder. ESI MS: m/z (rel intensity)374.02 (M⁻+H, 100), 391.03 (M⁺+NH₃, 70).

Example 18

[0233] a.(1N)-4-Methoxyphenylsulfonyl-(2R)-carbomethoxy-(4R)-benxyloxy-methoxy-pyrrolidine:Benzylchloromethylether (2.25 g, 9.54 mmole) is added dropwise to astirred solution of the methylester 1a (1.00 g, 3.18 mmole) in CH₂Cl₂(12 mL) and DIPEA (0.830 mL, 4.77 mmole) and stirred for four days.Additional CH₂Cl₂ is added and the mixture washed with saturatedNaH₂SO₄, dried over sodium sulfate and the solvent removed under vacuum.The dried material is purified over a silica column eluting first withhexane, then with hexane:EtOAc (7:3) to give a colorless oil. ESI MS:m/z (rel intensity) 436.07 (M⁺+H, 100), 453.09 (M⁺+NH₃, 70)

[0234] b.(1N)-4-Methoxyphenylsulfonyl-(2R)-N-hydroxycarboxamido-(4R)-benxyl-oxymethoxy-pyrrolidine:The starting methyl ester 18a (1.00 g, 2.29 mmole) is taken in 2 ml ofmethanol/tetrahydrofuran (1:1), and treated with NH₂OK (2 ml, 1.25M inmethanol) and stirred overnight. The following morning, dry silica (1.5ml) is added to the mixture and the solvent removed under vacuum. Thedry silica is poured on top of a flash silica column which issubsequently eluted with hexane: ethyl acetate (7:3) followed with ethylacetate to give a clear glass which is dried to a foamy solid by slightheating under vacuum. The product was recrystallized from cold methanolto give white powder. ESI MS: m/z (rel intensity) 436.98 (M⁻+H, 100),453.97 (M⁺+NH₃, 30).

Example 19

[0235] a.(1N)-4-Methoxyphenylsulfonyl-(2R)-carbomethoxy-(4R)-(2-methoxyethyl-oxy)-methoxypyrrolidine:MEM chloride (1.09 mL, 9.54 mmole) is added dropwise to a stirredsolution of the alcohol 1a (1.00 g, 3.18 mmole) in CH₂Cl₂ (12 mL) andDIPEA (0.830 mL) and stirred for 16 hrs. Additional CH₂Cl₂ is added andthe mixture is washed with saturated NaH₂SO₄, dried over sodium sulfateand the solvent removed under vacuum. The dried material was purifiedover a silica column eluting first with hexane:EtOAc (1:1) to give acolorless oil. ESI MS: m/z (rel intensity) 403.99 (M⁺+H, 70), 421.01(M⁺+NH₃, 100).

[0236] b.(1N)-4-Methoxyphenylsulfonyl-(2R)-carbomethoxy-(4R)-(2-methoxyethyl-oxy)-methoxypyrrolidine:The starting methyl ester 19 (450 mg, 1.12 mmole) is taken in 2 mL ofmethanol:tetrahydrofuran (1:1), and treated with NH₂OK (2 mL, 1.25M inmethanol) and stirred overnight. The following morning, dry silica (1.5mL) is added to the mixture and the solvent removed under vacuum. Thedry silica is poured on top of a flash silica column which issubsequently eluted with EtOAC followed with ethyl acetate:methanol(8:2) to give a clear glass which is puffed to a foamy solid by slightheating under vacuum. The product was recrystallized from coldEtOAc:hexane to give white powder. ESI MS: m/z (rel intensity) 405.05(M⁺+H, 100), 422.01 (M⁺+NH_(3, 20)).

Example 20

[0237] a.(1N)-4-Methoxyphenylsulfonyl-(2R)-carbomethoxy-(4S)-thioacetoxyl-pyrrolidine:Triphenylphosphine (0.9 g, 3.42 mmole) is dissolved in 12 mL of THF.Diethyl azodicarboxylate (0.54 mL, 3.42 mmole) is added dropwise at 0°C. After 30 min. with stirring, a solution of thioacetic acid (0.4 mL,5.13 mmole) and the alcohol 1a (0.54 g, 1.71 mmole) in 10 mL of THF isadded dropwise. The reaction is stirred at 0° C. for 30 min., roomtemperature for 2 hr and concentrated to an oil. The crude material ispurified by flash chromatography (CH₂Cl₂:hexane (1:1) to CH₂Cl₂:EtOAc;(50:1) to CH₂Cl₂:EtOAc; 25:1) on silica gel to give the desired product.ESI MS: m/z (rel intensity) 391 (100, M⁺+NH₃), 374 (65, M⁺+H).

[0238] b.(1N)-4-Methoxyphenylsulfonyl-(2R)-hydroxycarboxamido-(4S)-thio-pyrrolidine:The thioester 20a (0.4 g, 1.07 mmole) is dissolved in 2 mL of methanoland degassed by argon. A solution of NH₂OK (6.1 mL, 1.7 M in methanol,solution prepared as described in Fieser and Fieser, Vol 1, p 478) isalso degassed and added to the thioester solution. After 2 hr withstirring, the reaction is acidified with 1N HCl, concentrated to removesolvent, then distributed between HCl and ethyl acetate. The ethylacetate layer is washed with brine, dried over MgSO₄ and concentrated toan oil. The crude product is purified by flash chromatography (1% formicacid in EtOAc) on silica gel to give the desired product. ESI MS: m/z(rel intensity) 333 (90, M⁺+H).

Example 21

[0239] a.(±)-(1N)-4-Methoxyphenylsulfonyl-(2R)-carbomethoxy-(3S)-phenyl-pyrrolidine:(±)-trans-3-phenylproline (403 mg, 1.73 mmole, prepared as described inJ. Med. Chem. 1994, 37, 4371.) is dissolved in water:dioxane (1:1, 5 mL)with triethylamine (0.6 mL, 4.33 mmole). 4-Methoxyphenylsulfonylchloride (393 mg, 1.9 mmole) is added along with2,6-dimethylaminopyridine (catalytic) and the mixture is stirred 14 hr.at room temperature. The mixture is then concentrated and diluted withEtOAc. Layers are separated and the organic layer is washed 2× with 1NHCl, 1× with brine, dried over MgSO₄, filtered and evaporated to give623 mg of solid material which is dissolved in MeOH (15 mL). Thionylchloride (1.5 mL) is added dropwise and the resulting mixture stirredfor 14 hr. Silica gel (4 mL) is added and the mixture concentrated. Theresulting powder is poured onto a flash silica column and eluted withhexane: EtOAc (1:1 to 0:1) to give the title compound. ESI MS: m/z (relintensity) 376.1 (M⁺+H, 100), 316.1 (22).

[0240] b.(±)-(1N)-4-Methoxyphenylsulfonyl-(2R)-N-hydroxycarboxamido-(3S)-phenylpyrrolidine:The methylester 21a (0.262 g, 0.699 mmole) is taken in 1 mL of methanol,treated with NH₂OK (1.2 mL, 0.86 M in methanol, solution prepared asdescribed in Fieser and Fieser, Vol 1, p 478) and stirred overnight. Thefollowing morning, dry silica (2 mL) is added to the mixture and thesolvent removed under vaccuum. The dry silica is poured on the top of aflash silica gel column which is subsequently eluted withhexane:EtOAc:HCO₂H (2:1 to 0:1) to give pure white solid which isrecrystallized from CHCl₃:hexane (3:1) to give white crystals. ESI MS:m/z (rel intensity) 377.1 (M⁺+H, 100), 394.1 (M⁺+NH₃, 22).

Example 22

[0241] a.(1N)-4-Methoxyphenylsulfonyl-(2R)-carboxy-(4R)-hydroxy-pyrrolidine:cis-Hydroxy-D-proline (10 g, 0.38 mole) is dissolved in water: dioxane(1:1, 60 mL) with triethylamine (25 mL). 4-Methoxyphenylsulfonylchloride (17.4 g, 0.084 mole) is added along with2,6-dimethylaminopyridine (0.92 g, 0.008 mole) and the mixture isstirred 14 hr. at room temperature. The mixture is then concentrated anddiluted with EtOAc. Layers are separated and the organic layer is washed2× with 1N HCl, 1× with brine, dried over MgSO₄, filtered and evaporatedto give the title compound. ESI MS: 302.2 (M⁺+H, 100), 319.3 (M⁺+NH₄,85).

[0242] b. (1N)-4-Methoxyphenylsulfonyl-(2R)-carboxy-4-oxo-pyrrolidine: A0.76 M batch of Jones' reagent is prepared. The carboxyalcohol 22a (10.0g, 31.7 mmoles) is dissolved in 175 mL of acetone and cooled to 0° C.Jones reagent is added ( 420 mL, 317 mmoles) and this is stirred at roomtemperature for 14 hr. The reaction mixture is diluted with water andextracted 3× with EtOAc. The organic layers are washed 3× with water and1× with sodium chloride, dried over magnesium sulfate, and evaporated.The material is recrystallized from Hex:EtOAc to give the pure ketoacid.ESI MS: 300.3 (M⁺+H, 93), 317.3 (M⁺+NH₄, 100).

[0243] c.(1N)-4-Methoxyphenylsulfonyl-(2R)-carbomethoxy-(4,4)-(R)-hydroxyethylpyrrolidine:The ketone 22b (0.500 g, 1.67 mmole) is taken in 10 mL of THF and cooledto −15° C. Ethylmagnesium bromide (3.67 mL, 1M in TBF, 3.67 mmole) isadded to this mixture. The mixture is stirred for 30 min at which timeit is partitioned between 1N HCl and EtOAc. The organic layer is washedwith brine, dried over magnesium sulfate, filtered and evaparated. Thecrude material is then stirred overnight in methanol with 0.5 mL ofSOCl₂ and evaparated to dryness. The crude material is chromatographedover flash silica with hex:EtOAc (1:1) to give the pure title compound.ESI MS: 363.3 (M⁺+NH₄, 45), 346.3 (M⁺+H, 100).

[0244] d.(1N)-4-Methoxyphenylsulfonyl-(2R)-hydroxy-carboxamido-4,4)-(R)-hydroxyethylpyrrolidine:The methylester 22c (431 mg, 1.26 mmole) is taken in 1 mL of methanol,treated with NH₂OK (2 mL, 0.86 M in methanol, solution prepared asdescribed in Fieser and Fieser, Vol 1, p 478) and stirred overnight. Thefollowing morning, dry silica (2 mL) is added to the mixture and thesolvent removed under vaccuum. The dry silica is poured on the top of aflash silica gel column which is subsequently eluted withhexane:EtOAc:HCO₂H (2:1 to 0:1) to give pure white solid which isrecrystallized from CHCl₃:hexane (3:1) to give white crystals. ESI MS:362.2 (M⁺+NH₃, 60), 345.2 (M⁺+H, 100), 327.2 (15).

Example 23

[0245] a.(1N)-4-Methoxyphenylsulfonyl-(2R)-carbomethoxy-(4,4)-(R)-gem-hydroxyphenylpyrrolidine:The keto acid 22b (441 mg, 1.47 mmole) is treated with phenylmagnesiumbromide (3.7 mL, 3.7 mmole) as described for 22c to give a blackresidue. This is then treated with K₂CO₃ (760 mg, 5.5 mmole) and Mel(0.343 mL, 5.5 mmole) in 10 mL of DMF for 45 min. This mixture is thenpartitioned between EtOAc and brine. The organic layer is then driedover MgSO₄, filtered and evaporated. The crude residue is thenchromatographed over flash silica with hexane:EtOAc (9:1 to 7:3) to givethe title compound as a brown oil. CI⁺ MS: m/z (rel intensity) 409.4(M+NH₄ ⁺, 100), 392.4 (M⁺+H, 75), 374.4 (65), 204.2 (72).

[0246] b.(1N)-4-Methoxyphenylsulfonyl-(2R)-N-hydroxycarboxamido-(4,4)-(R)-gem-hydroxyphenylpyrrolidine:The ester 23a (174 mg, 0.445 mmole) is converted to the title hydroxamicacid as described for 22d. ESI MS: m/z (rel intensity) 410.6 (M+NH₄ ⁺,100), 393.4 (M⁺+H, 75), 375.5 (65).

Example 24

[0247] a.(1N)-4-Methoxyphenylsulfonyl-(2R)-carbomethoxy-4S)-4-octyl)phenoxycyclobutylamine: Triphenylphosphine (2.5 g, 9.51 mmole) was dissolved in20 mL of THF, diethyl azodicarboxylate (1.9 mL, 9.51 mmole) was addeddropwise at 0° C. After 30 min with stirring, a solution of4-octylphenol (2.46 g, 11.9 mmole) and the alcohol 1a (1.5 g, 4.76mmole) in 20 mL of THF was added dropwise. The reaction was stirred at0° C. for 30 min., room temperature overnight and concentrated to anoil. The crude product was purified by flash chromatography(hexane/EtOAc, 1:1) on silica gel to give the desired product. CI⁺ MS:m/z (rel intensity) 504 (44, M⁺+H), 334 (100).

[0248] b.(1N)-4-Methoxyphenylsulfonamido-(2R)-hydroxycarboxamido-(4S)-4-octyl)-phenoxypyrrolidine:The methyl ester 24a (1.1 g, 2.1 mmole) was taken in 1 mL of methanol,treated with NH₂OK (8 ml, 1.7 M in methanol, solution prepared asdescribed in Fieser and Fieser, Vol 1, p 478) and stirred for 30 hr.Silica (1.5 mL) was added to the mixture and the solvent was removedunder vacuum. The dry silica was poured on the top of a flash silica gelcolumn which was subsequently eluted with EtOAc:CH₃OH (95:5 to 90:10) togive 0.6 g (61% yield) of desired product as a white foamy solid. ESIMS: m/z (rel intensity) 527 (30, M⁺+Na), 522 (25, M⁺+NH₄), 505 (100,M⁻+H).

Example 25

[0249] a.(1N)-4-Methoxyphenylsulfonyl-(2R)-carbomethoxy-4-oxopyrrolidine: A 0.76M batch of jone's reagent was prepared. The alcohol 1a (10.0 g, 31.7mmoles) was dissolved in 175 mL of acetone and cooled to 0° C. Jone'sreagent was added (420 mL, 317 mmoles) and this was stirred at roomtemperature for 14 hr. The reaction mixture was diluted with water andextracted 3× with EtOAc. The organic layers were washed 3× with waterand 1× with sodium chloride, dried over magnesium sulfate, andevaporated. Chromotography was performed on silica gel usingEtOAc:hexane (1:1) to give pure compound. Starting material was alsorecovered. Cl⁺ MS: m/z (rel intensity) 314.0 (M⁺+H, 100).

[0250] b.(1N)-4-Methoxyphenylsulfonyl-(2R)-carbomethoxy-3,3-dimethyl-4-oxopyrrolidine:A solution of potassium bis(trimethylsilyl)amide (0.5 M, 10.2 mmole) in20.5 mL of toluene is cooled to 0° C. under argon atmosphere and chargedwith 10 mL of 1,3-dimethyl-3,4,5,6-tetrahydro-2(1H)-pyrimidone. Themixture is cooled to −78° C. A solution of the substrate 25a (800 mg,2.56 mmole) in 20 mL of THF is then added dropwise and the resultingmixture is stirred for 1 hr. Iodomethane (1.59 mL, 25.6 mmole) is thenadded and the reaction is stirred at −78° C. and then partitionedbetween EtOAc and dil. KHSO₄. The organic layer is then washed withbrine, dried over MgSO₄, filtered and evaperated. The crude oil is thenchromatographed over flash silica with hex:EtOAc (3:1 to 1:1) to givethe title compound. CI⁺ MS: m/z (rel intensity) 359 (M+NH₄ ⁺, 17), 342(M⁺+H, 20), 172 (100).

[0251] c.(1N)-4-Methoxyphenylsulfonyl-(2R)-carbomethoxy-3,3-dimethyl-(4R)-hydroxypyrrolidine:The starting ketone 25b (241 mg, 0.70 mmole) is taken in 5 mL ofmethanol and treated with NaBH₄ (42 mg, 1.05 mmole) at room temperature.The mixture is stirred at rt for 1 hr, quenced with 1N HCl, andpartitioned between 1N HCl and EtOAc. The mixture is then partitionedbetween 1N HCl and EtOAc. The organic layer is washed with brine, driedover MgSO₄, filtered and concentrated. The crude oil is chromatographedover flash silica to give the title compound as a clear syrup. The 1HNMR indicates a (10:1) diastereomeric mixture. ESI MS: m/z (relintensity): 346 (M⁺+H, 100), 363 (M⁺+NH₃)

[0252] d.(1N)-4-Methoxyphenylsulfonyl-(2R)-N-hydroxycarboxamido-3,3-methyl-(4R)-hydroxypyrrolidine:The starting ester 25c (90 mg, 0.26 mmole) is converted to the titlecompound as described for 22d. ESI MS: m/z (rel intensity): 345.2 (M⁺+H,100), 362.2 (M⁺+NH₃), 65), 383.1 (M⁺+K, 55).

Examples 26-41

[0253] The following examples W and Z are hydrogen, and Y is OH, n is 1,Ar is substituted or unsubstituted phenyl, and X and Q refer tosubstituents on the phenyl ring:

Example X Q Y 26 Me H a-OH 27 OMe 3-OMe a-OH 28 OMe 2-NO₂ a-OH 29O(n-Bu) H a-OH 30 O(n-Bu) H b-OH 31 Br H a-OH 32 Br 3-Me a-OH 33 Cl 2-Cla-OH 34 OCH₂CH₂OCH₃ H a-OH 35 OPh H a-OH 36 OCH(CH₃)₂ H a-OH 37 Br 2-Meb-S(3-C₆H₄OMe) 38 O(n-Bu) H b-2-mercaptobenzo-thiazole 39 OMe 2-NO₂b-2-mercaptobenzo-thiazole 40 O(n-Bu) H b-S(4-C₆H₄OMe) 41 O(n-Bu) HO-(3-pyridyl)

Example 26

[0254](1N)-4-Methylphenylsulfonyl-(2R)-carbomethoxy-(4R)-hydroxy-pyrrolidine:Cis-Hydroxy-D-proline methylester (303 mg, 2.09 mmole) is dissolved inDMF (3 mL) and N-methyl morpholine (1 mL) and stirred under air for 14hr at room temperature in the presence of p-toluenesulfonyl chloride(418 g, 2.19 mmole). The mixture is then partitioned between EtOAc and1N HCl. The layers are separated and the organic layer is washed 1× with1N HCl, 1× with brine, dried over MgSO₄, filtered and condensed to give341 mg of crude material which is chromatographed over flash silica withhexane:MeOH (19:1) to give the desired material as a white solid. CI⁺MS: m/z (rel intensity) 300 (M++H, 60), 240 (28), 146 (88), 126 (100).

[0255] b.(1N)-4-Methylphenylsulfonyl-(2R)-N-hydroxycarboxamido-(4S)-hydroxy-pyrrolidine:The methylester 26a (144 mg, 0.482 mmole) is taken in 1 mL of methanol,treated with NH₂OK (0.61 mL, 0.86 M in methanol, solution prepared asdescribed in Fieser and Fieser, Vol 1, p 478) and stirred overnight. Thefollowing morning the material is concentrated and partitioned betweenEtOAc and 1N HCl. The organic layer is washed with brine, dried overMgSO₄, filtered and evaparated to give 134 mg of crude material which ischromatographed over flash silica with EtOAc:MeOH (10:1) to give desiredproduct which is then recrystallized to give the desired white solid.ESI MS: m/z (rel intensity) 301.0 (M+H⁺, 100), 318.0 (M+NH₄ ⁺, 35),322.8 (M+Na⁺, 70).

Example 27

[0256] a.(1N)-3,4-Dimethoxyphenylsulfonyl-(2R)-carbomethoxy-(4R)-hydroxy-pyrrolidine:Cis-Hydroxy-D-proline methyl ester (2.71 g, 18.7 mmole) is dissolved inDMF (10 mL) and N-methyl morpholine (5 mL) and stirred under air for 14hr at room temperature in the presence of 3,4-dimethoxyphenyl-sulfonylchloride (4.65 g, 19.6 mmole). The mixture is then partitioned betweenEtOAc and 1N HCl. The layers are separated and the organic layer iswashed 1× with 1N HCl, 1× with brine, dried over MgSO₄, filtered andcondensed to give 3.98 g of crude material which is chromatographed overflash silica with hexane EtOAc (2:1 to 1:4) to give the desired materialas a white solid. CI⁺ MS: m/z (rel intensity) 346 (M⁺+H, 100), 286 (20),146 (15).

[0257] b.(1N)-3,4-Dimethoxyphenylsulfonyl-(2R)-N-hydroxycarboxamido-(4R)-hydroxypyrrolidine:The methylester 27a (250 mg, 0.724 mmole) is taken in 5 mL of methanol,treated with NH₂OK (1.25 mL, 0.86 M in methanol, solution prepared asdescribed in Fieser and Fieser, Vol 1, p 478) and stirred overnight. Thefollowing morning, dry silica (1.5 mL) is added to the mixture and thesolvent removed under vaccuum. The dry silica is poured on the top of aflash silica gel column which is subsequently eluted with hexane:EtOAc(1:1 to 0:1) to give a white foamy solid. ESI MS: miz (rel intensity)347.0 (M⁺+H, 100), 369.1 (M⁺+Na, 45).

Example 28

[0258] a.(1N)-2-Nitro-4-methoxyphenylsulfonyl)-(2R)-carbomethoxy-(4R)-hydroxypyrrolidine:cis-Hydroxy-D-proline (3.02 g, 23.1 mmole) is dissolved in water:dioxane(1:1,300 mL) with triethylamine (7.9 mL, 57.5 mmole).2-Nitro-4-methoxyphenylsulfonyl chloride (638 g, 25.4 mole) is addedalong with 2,6-dimethylaninopyridine (281 mg, 2.31 mmole) and themixture is stirred 14 hr. at room temperature. The mixture is thenconcentrated and diluted with EtOAc. Layers are separated and theorganic layer is washed 2× with 1N HCl, 1× with brine, dried over MgSO₄,filtered and evaparated to give 7.06 g of solid material which isdissolved in MeOH (100 mL). Thionyl chloride (10 mL) is added dropwiseand the resulting mixture stirred for 14 hr. The mixture is thenevaparated to dryness and triturated with CHCl₃ to give a brownish solidwhich is sufficiently pure to carry forward without purification. CI⁺MS: m/z (rel intensity) 378 (M+NH₄ ⁺, 40), 361 (M⁺+H, 100), 331 (12),301 (43), 144 (95).

[0259] b.(1N)-(2-Nitro-4-methoxyphenylsulfonyl)-(2R)-N-hydroxy-carboxamido-(4R)-hydroxypyrrolidine:The methylester 28a (300 mg, 0.833 mmole) is taken in 4 mL of methanol,treated with NH₂OK (1.44 mL, 0.86 M in methanol, solution prepared asdescribed in Fieser and Fieser, Vol 1, p 478) and stirred overnight. Thefollowing morning, dry silica (2 mL) is added to the mixture and thesolvent removed under vaccuum. The dry silica is poured on the top of aflash silica gel column which is subsequently eluted with hexane:EtOAc(4:1) then with EtOAc:MeOH:NH₄OH (8:2:0.1) to give a white solid. ESIMS: m/z (rel intensity) 362.0 (M⁺+H, 100), 379.2 (M⁺+NH₄ ⁺, 7), 384.1(M⁺+Na⁻, 55).

Example 29

[0260] a.(1N)-(4-^(n)Butoxyphenylsulfonyl)-(2R)-carbomethoxy-(4R)-hydroxy-pyrrolidine:Cis-D-Hydroxyproline methyl ester (583 mg, 4.02 mmole) is dissolved inDMF (7 mL) and N-methyl morpholine (3 mL) and stirred under air for 14hr at room temperature in the presence of para-n-butoxyphenylsulfonylchloride (1.00 g, 4.02 mmole). The mixture is then partitioned betweenEtOAc and 1N HCl. The layers are separated and the organic layer iswashed 1× with 1N HCl, 1× with brine, dried over MgSO₄, filtered andcondensed to give 1.2 g of crude material which is chromatographed overflash silica with hexane: EtOAc (4:1 to 1:3) to give the material as awhite solid. CI⁺ MS: m/z (rel intensity) 358 (M⁺+H, 100), 298 (23), 146(53), 114 (24).

[0261] b.(1N)-4-^(n)Butoxyphenylsulfonamido-2R)-N-hydroxycarboxamido-4R)-hydroxypyrrolidine:The methylester 29a (347 mg, 0.971 mmole) is taken in 2 mL of methanol,treated with NH₂OK (1.68 mL, 0.86 M in methanol, solution prepared asdescribed in Fieser and Fieser, Vol 1, p 478) and stirred overnight. Thefollowing morning, dry silica (2 mL) is added to the mixture and thesolvent removed under vaccuum. The dry silica is poured on the top of aflash silica gel column which is subsequently eluted with hexane:EtOAc(4:1) then with EtOAc:MeOH:NH₄OH (4:1:0.1) to give a white solid. ESIMS: m/z (rel intensity) 359.1 (M⁺+H, 100), 381.1 (M⁺+Na, 45).

Example 30

[0262] a.(1N)-4-Methoxyphenylsulfonyl-(2R)-carbomethoxy-(4S)-benzoyl-pyrrolidine:The alcohol 29a (200 mg, 0.56 mmole) is dissolved in 1.5 mL of methylenechloride. Benzoic acid (82 mg, 0.672 mmole) and triphenyl phosphine (220mg, 0.84 mmole) are then added, followed by diethyl azodicarboxylate(106 mL, 0.672 mmole). After 3 hrs, the reaction mixture is filtered andsilica gel is added to the filtrate to adsorb the solutes and themixture is concentrated to dryness. The resulting solid mixture ispoured onto the top of a flash silica column which is eluted with hexEtOAc (3:1 to 2:1) to give the desired product a white solid. CI⁺ MS:m/z (rel intensity) 479.1 (M+NH₄ ⁺, 55), 462.0 (M⁺+H, 30), 250.0 (100),126 (38).

[0263] b.(1N)-4-^(n)Butoxyphenylsulfonyl-(2R)-N-hydroxycarboxamido-(4S)-hydroxypyrrolidine:The methylester 30a (154 mg, 0.334 mmole) is taken in 2 mL of methanol,treated with NH₂OK (1.0 mL, 0.86 M in methanol, solution prepared asdescribed in Fieser and Fieser, Vol 1, p 478) and stirred overnight. Thefollowing morning, dry silica (1.5 mL) is added to the mixture and thesolvent removed under vaccuum. The dry silica is poured on the top of aflash silica gel column which is subsequently eluted with hexane:EtOAc(1:1 to 0:1) and finally with EtOAc:MeOH:NH₄OH (9:1:0.1) to give a clearglassy solid. ESI MS: m/z (rel intensity) 359 (M⁺+H, 40), 376 (M+NH₄ ⁺,30), 381 (M+Na⁺, 20).

Example 31

[0264] a.(1N)-4-Bromobenzenesulfonyl-(2R)-carbomethoxy-(4R)-hydroxypyrrolidine:The title ester was prepared as described for compound 28a fromcis-hydroxy-D-proline (4.43 g, 35.1 mmole) and 4-bromobenzenesulfonylchloride. ESI MS: m/z (rel intensity) 364.0 (M⁺+H, 95), 366.0 (M⁺+H,95), 381.0 (M⁺+NH₃, 98), 383.0 (M⁺+NH₃, 100).

[0265] b.(1N)-4-Bromobenzenesulfonyl-(2R)-N-hydroxycarboxamido-(4R)-hydroxy-pyrrolidine:The title hydroxamic acid was prepared from ester 31a (7.59 g, 20.9mmole) as described for compound 25. The resulting material wasrecrystallized from EtOAc. ESI MS: m/z (rel intensity) 365.1 (M⁺+H, 98),367.1 (M⁺+H, 100), 382.2 (M+NH₄ ⁺, 45), 384.2 (M+NH₄ ⁺, 45).

Example 32

[0266] a.(1N)-2-Methyl-4-bromobenzenesulfonyl-(2R)-carbomethoxy-(4R)-hydroxypyrrolidine:The title ester was prepared as described for compound 28a fromcis-hydroxy-D-proline (361 mg, 2.76 mmole) and2-methyl-4-bromobenzenesulfonyl chloride. CI⁺ MS: m/z (rel intensity)397 (M⁺+NH₃, 100), 395 (M⁺+NH_(3, 95)), 380 (M⁺+H, 50), 378 (M⁺+H, 45),317 (35), 300 (20), 146 (40).

[0267] b.(1N)-2-Methyl-4-bromobenzenesulfonyl-(2R)-hydroxycarboxamido-(4R)-hydroxypyrrolidine:The title hydroxamic acid was prepared from ester 32a (271 mg, 0.72mmole) as described for compound 28. The resulting material wasrecrystallized from water. ESI MS: m/z (rel intensity) 398 (M⁺+NH₃, 85),396 (M⁺+NH₃, 80), 379 (M⁺+H, 90), 381 (M⁺+H, 100).

Example 33

[0268] a. (1N)-2,4-Dichloro-(2R)-carbomethoxy-(4R)-hydroxypyrrolidine:The title compound is prepared as described for compound 28a fromcis-hydroxy-D-proline (500 mg, 3.8 mmole) and2,4-dichlorobenzenesulfonyl chloride (1.03 g, 4.2 mmole). ESI MS: m/z(rel intensity) 354.0 (M⁺+H, 100), 356.0 (M⁺+H, 73), 371.0 (M⁺+NH₄, 78),373.0 (M+NH₄, 54).

[0269] b.(1N)-2,4-Dichloro-(2R)-N-hydroxycarboxamido-(4R)-hydroxypyrrolidine: Thetitle compound is prepared from ester 33a (550 mg, 1.55 mmole) asdescribed for compound 28b. ESI MS: m/z (rel intensity) 355.1 (M⁻+H,100), 372.2 (M+NH₄ ⁺, 67).

Example 34

[0270] a. 4-(2-Methoxyethoxy)-phenylsulfonyl chloride: Methylsulfoxide(400 mL) is cooled with an ice/water bath with mechanical stirring andcharged with potassium hydroxide pellets (118.2 g, 2.11 mole) followedby phenol (94.1 g, 0.70 mole) and then 2-bromoethylmethyl ether (86 mL,0.9 mole) is added at a rapid dripping rate. The mixture is stirred for15 min., warmed to room temperature and then stirred for 2hrs. It isthen diluted with 1 L of ice/water and extracted 2 times with CH₂Cl₂.The combined organic layers were then dried over MgSO₄, filtered andevaparated The yield s in excess of 100% so it is taken in CHCl₃ andwashed 2 times with water and 1 time with brine. This organic layer wasprocessed similarly and the concentrate was taken in 1.1 L of CH₂Cl₂ ina mechanically stirred flask 5 L flask. Chlorosulfonic acid (140 mL, 2.1mole) is added dropwise causing slight warming A heavy precipitate isobserved after addition of half of the reagent, so the mixture isdiluted with 1.1 L of additional CH₂Cl₂. The resulting mixture isallowed to stir at rt for 16 hrs. It is then poured onto ˜2 L ofice/water. The layers are separated and the aqueous layer is extractedtwo times with CH₂C. The combined organic layers are then combined,dried over MgSO₄, filtered and evaparated to give the desired materialwhich is sufficiently pure to carry forward without purification. ESIMS: m/z (rel intensity) 247.1 (M⁺+H, 35), 264.1 (M⁺+NH₃, 100), 269.0(M⁺+Na, 45).

[0271] b.(1N)-4-(2-Methoxyethyl)phenylsulfonyl-(2R)-N-hydroxycarboxamido-(4R)-hydroxypyrrolidine:The title compound is prepared as described for compound 28a. ESI MS:m/z (rel intensity) 360.1 (M⁺+H, 85), 377.1 (M⁺+NH₄, 100).

[0272] c.(1N)-4-(2-Methoxyethyl)phenylsulfonyl-(2R)-N-hydroxycarboxamido-(4R)-hydroxypyrrolidine:The starting methylester 34b (347 mg, 0.971 mmole) is stirred overnightin 3 mL of methanol in the presence of NH₂OK (3.6 mL, 1.25 M inmethanol, solution prepared as described in Fieser and Fieser, Vol 1, p478). The solution is then partitioned between 0.1 N HCl and EtOAc. Theorganic layer is dried over MgSO₄, filtered and evaparated to give 710mg of a yellow solid which is chromatographed over flash silica withEtOAc:MeOH (1:0 to 5:1) to give the title compound which is puffed intoa solid white foam under vacuum. ESI MS: m/z (rel intensity) 361.1(M⁺+H, 100), 378.1 (M⁺+NH₄, 25).

Example 35

[0273] a.(1N)-4-Phenoxyphenylsulfonyl-(2R)-carbomethoxy-(4R)-hydroxypyrrolidine:The title compound is prepared from cis-D-hydroxyproline (5.00 g, 38.1mmole) and phenoxyphenylsulfonyl chloride (11.2 g, 42 mmole, prepared asdescribed for R. J. Cremlyn et al in Aust. J. Chem., 1979, 32, 445.52)as described for compound 28a. The compound is purified over flashsilica with EtOAc:hexane (1:1 to 1:0) to give the title compound as aclear gum. CI⁺ MS: m/z (rel intensity) 378.11 (M⁺+H, 100), 395.11(M⁺+NH₃, 40).

[0274] b.(1N)-4-Phenoxyphenylsulfonyl-(2R)-N-hydroxycarboxamido-(4R)-hydroxypyrrolidine:The starting methyl ester 35a (864 mg, 2.30 mmole) is taken in 6 mL ofmethanol:tetrahydrofuran (1:1), and treated with NH₂OK (3 mL, 1.25M inmethanol) and stirred overnight. The following morning, dry silica (1.5mL) is added to the mixture and the solvent removed under vacuum. Thedry silica is poured on top of a flash silica column which issubsequently eluted with ethyl acetate followed with ethylacetate:methanol (8:2) to give a clear glass which is puffed to a foamysolid by slight heating under vacuum. The product was recrystallizedfrom cold methanol to give the title compound as a white powder. ESI MS:m/z (rel intensity) 379.10 (M⁺+H, 100), 396.10 (M⁺+NH₃, 10).

Example 36

[0275] a. 4-(iso-butoxy)-phenylsulfonyl chloride: The title compound wasprepared as described for example 34a. ESI MS: m/z (rel intensity) 245.1(M⁺+H, 50), 262.1 (M⁺+NH₃, 100).

[0276] b.(1N)-4-iso-butyloxyphenylsulfonyl-(2R)-carbomethoxy-(4R)-hydroxypyrrolidine:The title ester was prepared from cis-hydroxy-D-proline (10.0 g, 76.3mmole) and sulfonyl chloride 36a (19.0 g, 76.3 mmole) as described forcompound 25a. ESI MS: m/z (rel intensity) 358.1 (M⁺+H, 100), 375.1(M⁺+Na, 45).

[0277] c.(1N)-4-iso-butyloxyphenylsulfonyl-(2R)-N-hydroxycarboxamido-(4R)-hydroxypyrrolidine:The starting methyl ester 36b (1.5 g, 4.2 mmole) is taken in 7 mL ofmethanol, and treated with NH₂OK (7 mL, 1.25M in methanol) and stirredovernight. A precipitate formed which is filtered and purified bypartioning between water and EtOAc. The organic layer is concentrated invacuo and recrystallized from hexane:EtOAc to give pure material. Theoriginal filtrate is dried and worked up like the filtrate and filteredthrough dry silica gel with EtOAc:MeOH (9:1) and the product wasrecrystallized from EtOAc:hexane to give additional product. ESI MS: m/z(rel intensity) 359.1 (M⁺+H, 100), 376.1 (M⁺+NH₄, 55), 381.1 (M⁺+Na,15).

Example 37

[0278] a.1N)-2-Methyl-4-bromophenylsulfonyl-(2R)-carbomethoxy-(4S)-3-methoxymercaptophenyl)-pyrrolidine:The starting alcohol 32a (310 mg, 0.82 mmole) is taken in 5 mL of CH₂Cl₂and 1 mL of triethylamine and treated with methanesulfonyl chloride (76μL, 0.984 mmole). The solution is stirred for 1 hr at rt and thenpartitioned between EtOAc and 1N HCl. The organic layer was dried overMgSO₄, filtered and evaparated. The crude residue was then taken in 2.5mL of THF at rt under argon and treated first with ^(t)butoxide (50 mg,0.45 mmole) and then 3-methoxythiophenol (110 μL, 0.90 mmole) Themixture is stirred for 16 hr and then partitioned between EtOAc and 1NHCl. The organic layer is washed with brine, dried over Na₂SO₄, filteredand evaparated to give a residue which is then chromatographed overflash silica with hexane:EtOAc (4:1) to give the title compound as aclear glass. CI⁺ MS: m/z (rel intensity) 517, 519 (M+NH₃, 92), 500, 502(M⁺+H, 48) 439 (30), 422 (20), 141 (50), 128 (100).

[0279] b.(1N)-2-Methyl-4-bromophenylsulfonyl-(2R)-N-hydroxycarboxamido-(4S)-(3-methoxymercapto-phenyl)-pyrrolidine:The methylester 37a (101 mg, 0.202 mmole) is taken in 2 mL ofmethanol:THF (1:1), treated with NH₂OK (2.0 mL, 1.25 M in methanol,solution prepared as described in Fieser and Fieser, Vol 1, p 478) andstirred overnight. The following morning, dry silica (1.5 mL) is addedto the mixture and the solvent removed under vaccuum. The dry silica ispoured on the top of a flash silica gel column which is subsequentlyeluted with EtOAc and then with EtOAc:MeOH (4:1) to give 79 mg (79%) ofa clear glassy solid. ESI MS: m/z (rel intensity) 501, 503 (M⁺+H, 65),518, 520 (M⁺+NH₃, 100), 523, 525 (M⁺+Na, 35).

Example 38

[0280] a.(1N)-^(n)Butoxyphenylsulfonyl-(2R)-N-hydroxycarboxamido-(4S)-(2-mercaptobenzothiazolyl)-pyrrolidine:The alcohol 29a (200 mg, 0.56 mmole) is dissolved in 2.5 mL of methylenechloride. 2-Mercaptobenzothiazole (113 mg, 0.672 mmole) andtriphenyl-phosphine (220 mg, 0.84 mmole) are then added, followed bydiethyl azodicarboxylate (106 mL, 0.672 mmole). After 3 hrs, thereaction mixture is filtered and silica gel is added to the filtrate toadsorb the solutes and the mixture is concentrated to dryness. Theresulting solid mixture is poured onto the top of a flash silica columnwhich is eluted with hex:EtOAc (2:1 to 1:1) to give the desired product.MS CI⁺: m/z (rel intensity) 507.0 (M+H⁺, 30), 359.1 (42), 342.0 (39),167.9 (100), 135.9 (90).

[0281] b.(1N)-4-^(n)Butoxyphenylsulfonyl-(2R)-N-hydroxycarboxamido-(4S)-(2-mercaptobenzothiazolyl)-pyrrolidine:The methylester 38a (214 mg, 0.422 mmole) is taken in 1.5 mL ofmethanol, treated with NH₂OK (0.73 mL, 0.86 M in methanol, solutionprepared as described in Fieser and Fieser, Vol 1, p 478) and stirredovernight. The following morning, dry silica (2 mL) is added to themixture and the solvent removed under vaccuum. The dry silica is pouredon the top of a flash silica gel column which is subsequently elutedwith hexane:EtOAc (1:1 to 0:1) and finally with EtOAc:MeOH:NH₄OH(4:1:0.1) to give a white powder. ESI MS: m/z (rel intensity) 508 (M⁺+H,100), 532 (M⁺+Na, 32).

Example 39

[0282] a.(1N)-2-Nitro-4-methoxyphenylsulfonyl-(2R)-N-carbomethoxy-(4S)-(2-mercaptobenzothiazolyl)-pyrrolidine:The alcohol 28a (200 mg, 0.55 mmole) is dissolved in 1.5 mL of methylenechloride. 2-Mercaptobenzothiazole (112 mg, 0.66 mmole) and triphenylphosphine (219 mg, 0.833 mmole) are then added, followed by diethylazodicarboxylate (105 mL, 0.666 mmole). After 3 hrs, the reactionmixture is filtered and silica gel is added to the filtrate to adsorbthe solutes and the mixture is concentrated to dryness. The resultingsolid mixture is poured onto the top of a flash silica column which iseluted with hex:EtOAc (4:1 to 1:1) to give the desired product as awhite solid. CI⁺ MS: m/z (rel intensity) 509.9 (M⁺+H, 30), 315.0 (18),294.9 (18), 167.9 (100), 135.9 (95).

[0283] b.(1N)-2-Nitro-4-methoxyphenylsulfonyl-(2R)-N-hydroxy-carboxamido-(4S)-(2-mercapto-benzothiazolyl)-pyrrolidine:The methylester 39a (277 mg, 0.544 mmole) is taken in 1 mL of methanol,treated with NH₂OK (1.0 mL, 0.86 M in methanol, solution prepared asdescribed in Fieser and Fieser, Vol 1, p 478) and stirred overnight. Thefollowing morning, dry silica (2 mL) is added to the mixture and thesolvent removed under vaccuum. The dry silica is poured on the top of aflash silica gel column which is subsequently eluted with hexane:EtOAc(1:1 to 0:1) followed by EtOAc:MeOH:NH₄OH (9:1:0.1) to give the whitesolid. ESI MS: m/z (rel intensity) 511.1 (M⁺+H, 100), 533.0 (M⁺+Na, 30).

Example 40

[0284] a.(1N)-(4-^(n)Butoxyphenylsulfonyl)-(2R)-carbomethoxy-(4S)-(4-methoxymercaptophenyl)-pyrrolidine:The alcohol 29a (178 mg, 0.499 mmole) is taken in 2 mL of CH₂Cl₂ and tothis mixture is added triphenylphosphene (157 mg, 0.599 mmole),4-methoxythiophenol (67 mL, 0.548 mmole), and diethyl-diazadicarboxylate(95 mM, 0.0.548 mmole) and the mixture is stirred for 3 hr. at whichtime 3 mL of silica gel is added to the mixture which is thenconcentrated to dryness. The dry residue is poured onto the top of aflash silica column and eluted with hexane:EtOAc (4:1 to 1:4) to give aclear oil. CI⁺ MS: m/z (rel intensity) 468 (M⁺+H, 48), 301 (43), 272(46), 187 (65), 109 (100).

[0285] b.(1N)-(4-Methoxyphenylsulfonyl)-(2R)-N-hydroxycarboxamido-(4S)-4-methoxyphenylthioloxy)-pyrrolidine:The methylester 40a (125 g, 0.268 mmole) is taken in 1 mL of methanol,treated with NH₂OK (0.465 mL, 0.86 M in methanol, solution prepared asdescribed in Fieser and Fieser, Vol 1, p 478) and stirred overnight. Thefollowing morning, dry silica (2 mL) is added to the mixture and thesolvent removed under vaccuum. The dry silica is poured on the top of aflash silica gel column which is subsequently eluted with hexane:EtOAc(2:1 to 0:1) to give a white solid. ESI MS: m/z (rel intensity) 481(M⁺+H, 10), 498.1 (M+NH₄ ⁺, 100), 503.1 (M⁺+Na, 20).

Example 41

[0286] a.(1N)-4-^(n)Butoxyphenylsulfonyl-(2R)-carbomethoxy-(4S)-(3-pyridyloxy)-pyrrolidine:The title compound is prepared as described for 13a. CI⁺ MS: m/z (relintensity) 468 (M⁺+H, 48), 301 (43), 272 (46), 187 (65), 109 (100).

[0287] b.(1N)-4-^(n)Butoxyphenylsulfonyl-(2R)-N-hydroxycarboxamido-(4S)-(3-pyridyloxy)-pyrrolidine:The title compound is prepared as described for 13b. ESI MS: m/z (relintensity) 436.1 (M⁻+H, 100), 458.1 (M+NH₄ ⁺, 60), 517.8 (M⁻+Na, 15).

Examples 42-61

[0288] In the following examples W and Z are hydrogen, and Y is OH, n is1, Ar is substituted or unsubstituted phenyl, and X and Q refer tosubstituents on the phenyl ring:

Example X P R 42 OMe H H 43 OnBu H H 44 OMe H n-Pr 45 OMe H n-Hex 46 OMeH CH₂CH₂Ph 47 OMe n-Bu n-Hex 48 OMe H SO₂Me 49 On—Bu H SO₂Me 50 On—Bu HSO₂3-(N- methylimidazole) 51 OMe CH₂(3- SO₂Me pyridyl) 52 OMe SO₂MeSO₂Me 53 OMe n-Pr SO₂Me 54 OMe H SO₂pC₆H₄OMe 55 OMe H COn-Pent 56 OMe HCOp-Ph—Ph 57 OMe H CONHMe 58 OMe H COCH(R—OBn)CH₃ 59 OMe HCOCH(R—OBn)CH₂Ph 60 OMe i-Pr COCH(R—OH)CH₃ 61 OMe i-Pr COCH(R—OH)CH₂Ph

Example 42

[0289] a.(1N)-4-Methoxyphenylsulfonyl-(2R)-carbomethoxy-(4S)-azidopyrrolidine:The starting mesylate 15a (4.2 g, 10.7 mmole) is taken in 15 mL of dryDMF in the presence of NaN₃ (695 mg, 10.7 mmole). The resulting mixtureis heated to 55° C. for 26 hrs and then partitioned between water andEtOAc. The organic layer is then washed with brine, dried over MgSO₄,filtered and evaparated. The resulting crude oil is chromatographed overflash silica with hexane:EtOAc (5:1 to 3:1) to provide pale yellow oilwhich solidifies upon standing. CI⁺ MS: m/z (rel intensity) 358 (M+NH₄⁺, 50), 341 (M⁺+H, 67), 315 (95), 145 (100).

[0290] b.(1N)-4-Methoxyphenylsulfonyl-(2R)-carbomethoxy-(4S)-aminopyrrolidine:The starting azide 42a (1.18 g, 3.48 mmole), is taken in 100 mL ofEtOH:THF:HCO₂H (5:1:0.1), and hydrogenated at rt. under 54 psi ofhydrogen in the presence of 100 mg of 10% Pd-C for 16 hrs. The mixtureis then filtered through a pad of celite, concentrated to an oil andrecrystallized from hexane: EtOAc to give the desired product as theformate salt. CI⁺ MS: m/z (rel intensity) 315 (M⁺+H, 12), 177 (13), 143(42), 123 (60), 109 (100).

[0291] c.(1N)-4-Methoxyphenylsulfonyl-(2R)-N-hydroxycarboxamido-(4S)-aminopyrrolidine:The starting ester 42b (500 mg, 1.59 mmole), is taken in 5 mL of MeOH,treated with NH₂OK (1.92 mL, 0.86 M in methanol, solution prepared asdescribed in Fieser and Fieser, Vol 1, p 478) and stirred overnight. Thefollowing morning, dry silica (1.5 mL) is added to the mixture and thesolvent removed under vaccuum. The dry silica is poured on the top of aflash silica gel column which is subsequently eluted with EtOAc:MeOH(4:1 to 3:2) to give white solid. ESI MS: m/z (rel intensity) 316.3(M⁺+H, 100), 333.3 (M⁺+NH₄, 15).

Example 43

[0292] a.(1N)-4-^(n)Butoxyphenylsulfonyl-(2R)-carbomethoy-(4R)-methylsulfonoxy-pyrrolidine:The starting alcohol 1a (6.78 g, 19.0 mmole) is converted to the titlemesylate as described for compound 15a. CI MS: m/z (rel intensity) 453(M+NH₄ ⁺, 38), 336 (M⁺+H, 27), 224 (100), 128 (67).

[0293] b.(1N)-4-^(n)Butoxyphenylsulfonyl-(2R)-carbomethoxy-(4S)-azidopyrrolidine:The starting mesylate 43a (5.85 g, 13.5 mmole) is converted to the titleazide as described for compound 41a. ESI MS: m/z (rel intensity) 383.1(M⁺+H, 50), 400.1 (M⁺+NH₃, 100)

[0294] c.(1N)-^(n)Butoxyphenylsulfonyl-(2R)-carbomethoxy-(4S)-aminopyrrolidine:The starting azide 43b (4.65 g, 12.2 mmole), is taken in 200 mL of MeOHwith 20 mL of HOAc and hydrogenated at rt. under 54 psi of hydrogen inthe presence of 200 mg of 10% Pd-C for 16 hrs. The mixture is thenfiltered through a pad of celite, concentrated to an oil, taken in MeOHand stirred with −50 g of Amberlite IRA-68 basic resin (preconditionedwith 0.1 N NaOH, water and MeOH), filtered through a glass frit andadsorbed onto a plug of silica. This is then eluted over a column offlash silica with EtOAc:MeOH (1:0 to 3:1) to give pale yellow oil whichsolidifies upon standing. CI MS: m/z (rel intensity) 357 (M⁺+H, 65), 145(100).

[0295] d.(1N)-4-Methoxyphenylsulfonyl-(2R)-hydroxycarboxamido-(4S)-amino-pyrrolidine:The starting ester 43c (234 mg, 356 mmole), is converted to the titlecompound as described for compound 42c and then purified further byrecrystallizing from water to give white crystalls. ESI MS: m/z (relintensity) 358 (M⁺+H).

Example 44

[0296] a.(1N)-4-Methoxyphenylsulfonyl-(2R)-carbomethoxy-(4S)-propylamino-pyrrolidine:The starting amine 42b (810 mg, 2.6 mmole) is dissolved in 8 mL ofmethanol and stirred for 48 hrs in the presence of propianaldehyde (206mL, 2.86 mmole), sodium cyanoborohydride (180 mg, 2.86 mmole), sodiumacetate (810, 9.9 mmole) and 25 drops of acetic acid. The mixture isevaparated to dryness and then partitioned between dil. NaHCO₃ and EtOAcand the organic layer is washed 2 times with NaHCO₃, 1 time with brine,dried over MgSO₄, filtered and evaparated to give a gummy oil which wassufficiently clean to carry forward without further purification. ESIMS: m/z (rel intensity) 357.3 (M⁺+H, 100).

[0297] b.(1N)-4-Methoxyphenylsulfonyl-(2R)-N-hydroxycarboxamido-(4S)-propylaminopyrrolidine:The starting methylester 44a (11.3, g, 31.7 mmole) is taken in 30 mL ofmethanol, treated with NH₂OK (38 mL, 1.25 M in methanol, solutionprepared as described in Fieser and Fieser, Vol 1, p 478) and stirredfor 16 hrs. The following morning, dry silica (30 mL) is added to themixture and the solvent removed under vaccuum. The dry silica is pouredon the top of a flash silica gel column which is subsequently elutedwith chloroform:methanol (8:2) to give a pale yellow solid which wastaken in methanol and stirred for 1 hr in the presence of activatedcharcoal and then filtered through celite and evaparated to give a whitesolid. ESI MS: m/z (rel intensity) 358.2 (M⁺+H, 100), 380.1 (M⁺+Na, 5).

Example 45

[0298] a.(1N)-4-Methoyphenylsulfonyl-(2R)-carbomethoxy-(4S)-^(n)hexylamino-pyrrolidine:The starting alcohol 1a (300 mg, 0.951 mmole) is dissolved in 2 mL ofCH₂Cl₂ under argon and cooled to 0° C. 2,6-Lutidine (135 μL, 1.14 mmole)is added via syringe followed by like addition oftrifluoro-methanesulfonyl anhydride (179 mL, 1.05 mmole). The mixture isstirred for 1 hr., followed by syringe addition of dry hexylamine (500μL, 3.80 mmol) and then the mixture is allowed to come to roomtemperature, stir for 14 hrs., and heat to reflux for 4 hrs. Silica gel(3 mL) is added and the mixture evaparated to dryness. The dry powder ispoured on the top of a column of flash silica gel which is then elutedwith hexane:EtOAc (2:1Ø1:1) to give a colorless, glassy solid. CI⁺ MS:m/z (rel intensity) 399 (M⁺+H, 38), 229 (100), 227 (62).

[0299] b.(1N)-4-Methoxyphenylsulfonyl-(2R)-N-hydroxycarboxamido-(4S)-(^(n)hexylamino)-pyrrolidine:The starting methylester 45a (88 mg, 0.221 mmole) is taken in 1 mL ofmethanol, treated with NH₂OK (0.381 mL, 0.86 M in methanol, solutionprepared as described in Fieser and Fieser, Vol 1, p 478) and stirredovernight. The following morning, dry silica (1.5 mL) is added to themixture and the solvent removed under vaccuum. The dry silica is pouredon the top of a flash silica gel column which is subsequently elutedwith hexane:EtOAc (1:1Ø0:1) to give a white foamy solid. ESI MS: m/z(rel intensity) 400.3 (M⁻+H, 100), 422.2 (M⁺+Na, 12).

Example 46

[0300] a.(1N)-4-Methoxyphenylsulfonyl-(2R)-carbomethoxy-(4S)-2-phenylethyl-amino-pyrrolidine:The primary amine 42b (300 mg, 1 mmole) is N-alkylated withphenylacetaldehyde (0.13 mL, 1.1 mmole) as described for compound 44a togive the desired amine as a clear gum which was carried forward withoutfurther purification. CI⁺ MS: m/z (rel intensity) 419 (M⁺+H, 38), 249(20), 249 (19).

[0301] b.(1N)-4-Methoxyphenylsulfonyl-(2R)-N-hydroxycarboxamido-(4S)-2-phenylethyl-aminopyrrolidine:The starting ester 46a (490 mg, 1 mmole) is converted to the titlecompound as described for compound 45b and purified over flash silicawith EtOAc:MeOH (4:1) to give a white solid. ESI MS: n/z (rel intensity)420.4 (M⁺+H, 100).

Example 47

[0302] a.(1N)-4-Methoxyphenylsulfonyl-(2R)-carbomethoxy-(4S)-N,N-^(n)butyl,^(n)hexylamino-pyrrolidine:The starting amine 45a (100 mg, 0.251 mmole) was converted to 93 mg(82%) of the title compound as described for compound 44a. CI⁺ MS: m/z(rel intensity) 470 (M⁺+H, 10), 299 (20), 242 (100).

[0303] b.(1N)-4-Methoxyphenylsulfonyl-(2R)-N-hydroxycarboxamido-(4S)-N,N-^(n)butyl,^(n)hexylamino-pyrrolidine:The starting ester 47a (80.5 mg, 0.172 mmole) was converted to 56 mg(69%) of the title compound as described for compound 44b. CI⁺ MS: m/z(rel intensity) 469 (M⁺+H, 42), 299 (100), 242 (28), 172 (46).

Example 48

[0304] a.(1N)-4-Methoxyphenylsulfonyl-(2R)-carbomethoxy-(4S)-methanesulfonyl-aminopyrrolidine:The primary amine 42b (502 mg, 1.60 mmole) is taken in 5 mL of methylenechloride and 0.5 mL of triethyl amine and treated with methanesulfonylchloride (200 μL, 2.58 mmole) via syringe. The mixture is stirred for 2hr and then partitioned between 1N HCl and EtOAc. The organic layer waswashed with brine, dried over MgSO₄, filtered and evaparated to give 684mg of crude material which was chromatographed over flash silica withhexane EtOAc (2:1 to 1:1) to give disulfonylated material 51a andmonosulfonylated material 47a. CI⁺ MS: m/z (rel intensity) 410 (M⁺+NH₄,15), 393 (M⁺+H, 10), 203 (100).

[0305] b.(1N)-4-Methoxyphenylsulfonyl-(2R)-hydroxycarboxamido-(4S)-methane-sulfonylaminopyrrolidine:The starting ester 48a (354 mg, 0.903 mmole) is converted to the titlecompound and chromatographed as described for compound 45b. It is thenrecrystallized from acetonitrile/water to give pale yellow crystalls.ESI MS: m/z (rel intensity) 394 (M⁺+H, 60), 411 (M⁺+NH₄, 100).

Example 49

[0306] a.(1N)-4-^(n)Butoxyphenylsulfonyl-(2R)-carbomethoxy-(4S)-methanesulfonyl-aminopyrrolidine:The primary amine 43c (21.3 g, 60 mmole) is taken in 120 mL of methylenechloride and 36 mL of triethyl amine and treated dropwise withmethanesulfonyl chloride (5.1 mL, 66 mmole) at 0° C. The mixture isallowed to come to room temperature for 1 hr and then adsorbed ontosilica, evaporated to dryness, and eluted through a column of flashsilica with hexane: EtOAc (4:1 to 1:1) to give the title compound. ESIMS: m/z (rel intensity) 452 (M⁺+NH₃, 12), 435 (M⁺+H, 9), 223 (100).

[0307] b.(1N)-4-^(n)Butoxyphenylsulfonyl-(2R)-N-hydroxycarboxamido-(4S)-methane-sulfonylaminopyrrolidine:The starting ester 49a (21.4 g, 49.2 mmole) is taken in 60 mL ofmethanol:THF (1:1), treated with NH₂OK (59 mL, 1.25 M in methanol,solution prepared as described in Fieser and Fieser, Vol 1, p 478) andstirred overnight. The following morning, dry silica (45 mL) is added tothe mixture and the solvent removed under vaccuum. The dry silica ispoured on the top of a flash silica gel column which is subsequentlyeluted with hexane:EtOAc (1:1Ø0:1), then EtOAc:methanol (9:1) to give awhite foamy solid. This material was heated to 60° C. for 48 hrs and awhite, solid impurity sublimed off leaving behind light yellow powder.ESI MS: m/z (rel intensity) 453.08 (M⁺+NH₃, 50), 436.05 (M⁺+H, 100).

Example 50

[0308] a.(1N)-4-n-Butoxyphenylsulfonyl-(2R)-carbomethoxy-(4S)-[(1N)-methyl-3-imidazolyl]-sulfonylamino-pyrrolidine:The primary amine 43c (232 mg, 0.906 mmole) is taken in 3 mL ofmethylene chloride and 0.5 mL of triethyl amine and treated with1N-methyl-3-imidazoyl-sulfonyl chloride (280 mg, 1.55 mmole) at rt. Themix e is allowed to stir for 16 hr and then adsorbed onto silica,evaparated to dryness, and eluted through a column of flash silica withhexane:EtOAc (1:1 to 0:1) to give the title compound as a clear oilwhich contained ˜20 mole percent of the starting sulfonyl chloride. Thismaterial was carried forward without further purification. ESI MS: m/z(rel intensity) 501 (M⁺+F, 70), 357 (45), 289 (82), 162 (100).

[0309] b.(1N)-4-n-Butoxyphenylsulfonyl-(2R)-N-hydroxycarboxamido-(4S)-[(1N)-methyl-3-imidazolyl]-sulfonylamino-pyrrolidine:The starting ester 50a (236 mg, 0.471 mmole) is converted to the titlecompound and chromatographed as described for compound 45b to give 262mg of yellow oil which was further purified by reverse phase prep. HPLCto give pure solid. ESI MS: m/z (rel intensity) 502.2 (M⁺+H).

Example 51

[0310] a.(1N)-4-Methoxyphenylsulfonyl-(2R)-carbomethoxy-(4S)-N-(3-pyridyl)-methylaminopyrrolidine:The primary amine 42b (810 mg, 2.6 mmole) is N-alkylated with3-pyridine-carboxaldehyde (270 μL, 2.86 mmole) as described for compound44a to give the desired amine as a clear gum which is purified overflash silica gel with EtOAc:MeOH (1:0 to 9:1) to give white solid. CIMS: m/z (rel intensity) 406 (M⁺+H, 100), 236 (45), 234 (48).

[0311] b.(1N)-4-Methoxyphenylsulfonyl-(2R)-carbomethoxy-(4S)-N,N-(3-pyridylmethyl)-(methanesulfonyl)-aminopyrrolidine:The secondary amine 51a (7.80 mg, 19.3 mmole) is taken in 85 mL ofmethylene chloride and 11 mL of triethyl amine with a catalytic amountof 2,5-dimethylamino-pyridine and treated with methanesulfonyl chloride(4.5 mL, 57.8 mmole) at rt. The mixture is allowed to stir for 16 hr andthen adsorbed onto silica, evaparated to dryness, and eluted through acolumn of flash silica with EtOAc:MeOH (0:1 to 9:1) to give the titlecompound as a yellow foamy solid. CI MS: m/z (rel intensity) 484 (M⁺+H,30), 406 (10), 314 (40), 234 (90), 187 (42), 102 (100).

[0312] c.(1N)-4-Methoxyphenylsulfonyl-(2R)-N-hydroxycarboxamido-(4S)-N,N-(3-pyridylmethyl)-(methanesulfonyl)-aminopyrrolidine:The staring ester 51b (6.33 g, 13.1 mmole) is converted to the relativehydroxamic acid as described for compound 45b and eluted through flashsilica with EtOAc:MeOH (1:0 to 4:1) to give the title compound as awhite powder. ESI MS: m/z (rel intensity) 484.9 (M⁺+H, 100), 506.9(M⁺+NH₃, 10).

Example 52

[0313] a.(1N)-4-Methoxyphenylsulfonyl-(2R)-carbomethoxy-(4S)-bis-(N-methanesulfonyl)-amino-pyrrolidine:The title compound is isolated from the crude mixture in 48a. ESI MS:m/z (rel intensity) 488.3 (M⁺+NH₄ ⁺, 15), 471.3 (M⁺+H, 10).

[0314] b.(1N)-4-Methoxyphenylsulfonyl-(2R)-N-hydroxycarboxamido-(4S)-bis-N-methanesulfonyl)-amino-pyrrolidine:The starting ester 52a (94 mg, 0.20 mmole) is converted to the relativehydroxamic acid as described for compound 48b and eluted through flashsilica with EtOAc:MeOH (1:0 to 5:1) to give the title compound as awhite solid. ESI MS: m/z (rel intensity) 489.3 (M⁺+NH₄ ⁺, 55), 472.3(M⁺+H, 100).

Example 53

[0315] a.(1N)-4-Methoxyphenylsulfonyl-(2R)-carbometboxy-(4S)-N-(methane-sulfonyl)-propyl-aminopyrrolidineThe starting amine 44a (783 mg, 2.20 mmole) was converted to the titlecompound as described for 48a. ESI MS: m/z (rel intensity) 452 (M+NH₄+),435 (M⁺+H, 75), 265 (100), 155 (75), 126 (40).

[0316] b.(1N)-4-Methoxyphenylsulfonyl-(2R)-N-hydroxycarboxamido-(4S)-N-(methanesulfonyl)-propyl-aminopyrrolidineThe starting ester 53a (614 mg, 1.41 mmole) was converted to the titlecompound as described for 48b. ESI MS: m/z (rel intensity) 452.9 (M+NH₄⁺, 100), 435.8 (M⁺+H, 55).

Example 54

[0317] a.(1N)-4-Methoxyphenylsulfonyl-(2R)-carbomethoxy-(4S)-4-methoxyphenyl-sulfonylamino-pyrrolidine:The primary amine 42b (400 mg, 1.27 mmole) is converted to the titlecompound with p-methoxybenzenesulfonyl chloride (316 mg, 1.53 mmole) asdescribed for compound 48a. CI⁺ MS: m/z (rel intensity) 502 (M⁺+NH₄ ⁺,12), 485 (M⁺+H, 10), 315 (100).

[0318] b.(1N)-4-Methoxyphenylsulfonyl-(2R)-N-hydroxycarboxamido-(4S)-4-methoxyphenyl-sulfonylamino-pyrrolidine:The starting ester 54a (480 mg, 0.99 mmole) is converted to the relativehydroxamic acid as described for compound 48b and eluted through flashsilica with EtOAc:MeOH:HCO₂H (1:0:0 to 4:1:0.1) to give the titlecompound as a white solid which was recrystallized from acetonitrile:water to give white crystalls. ESI MS: m/z (rel intensity) 486 (M⁺+H,100), 503 (M⁺+NH₄, 30).

Example 55

[0319] a.(1N)-4-Methoxyphenylsulfonyl-(2R)-carbomethoxy-(4S)-(-1-oxyhexyl)-aminopyrrolidine:The primary amine 42b (500 mg, 1.59 mmole) is converted to the titlecompound with hexanoyl chloride (268 mL, 1.91 mmole) as described forcompound 48a. ESI MS: m/z (rel intensity) 413.2 (M⁺+H, 70), 430.2(M⁺+NH₄, 100).

[0320] b.(1N)-4-Methoxyphenylsulfonyl-(2R)-N-hydroxycarboxamido-(4S)-(-1-oxyhexyl)-aminopyrrolidine:The starting ester 55a (560 mg, 1.35 mmole) is converted to the relativehydroxamnic acid as described for compound 48b and eluted through flashsilica with EtOAc:MEOH:HCO₂H (1:0:0 to 4:1:0.1) to give the titlecompound as a pale orange, viscous sap which would not solidify. ESI MS:m/z (rel intensity) 431.4 (M⁺+NH₄ ⁺, 25), 414.4 (M⁺+H, 35), 102 (100).

Example 56

[0321] a.(1N)-Methoxyphenylsulfonyl-(2R)-carbomethoxy-)4S)-p-biphenylyl-aminopyrrolidine:The primary amine 42b (1.00 g, 3.19 mmole) is converted to the titlecompound with 4-biphenyl chloride (761 mg, 3.51 mmole) as described forcompound 48a. CI⁺ MS: m/z (rel intensity) 4.95 M⁺+H, 30), 325 (100), 198(55), 155 (27).

[0322] b.(1N)-4-Methoxyphenylsulfonyl-(2R)-hydroxycarboxamido-(4S)-p-biphenylylaminopyrrolidine:The starting ester 56a (200 mg, 0.404 mmole) is converted to therelative hydroxamic acid as described for compound 48b and elutedthrough flash silica with EtOAc:MeOH(1:0:0 to 9:1) to give 129 mg (65%)of the title compound. ESI MS: m/z (rel intensity) 496.0 (M⁺+H, 100),513.0 (M+NH₄ ⁺, 60), 517.8 (M⁺+Na, 15).

Example 57

[0323] a.(1N)-4-Methoxyphenylsulfonyl-(2R)-carbomethoxy-(4S)-methylcarboxamyl-aminopyrrolidine:The primary amine 42b (470 mg, 1.49 mmole) is taken in 4 mL of dioxanewith 1 mL of triethyl amine and a catalytic amount of DMAP and thentreated with methyl isocyanate (106 μL, 1.80 mmole) and stirred for 16hrs at rt. The mixture is then partitioned between EtOAc and 1N HCl andthe organic layer is washed with brine, dried over MgSO₄, filtered andevaparated. The residue is then chromatographed over flash silica withhexane:EtOAc (1:2 to 0:1) to give white solid. CI⁺ MS: m/z (relintensity) 389 (M⁺+NH₄ ⁺, 5), 372 (M⁺+H, 25), 202 (100).

[0324] b.(1N)-4-Methoxyphenylsulfonyl-(2R)-hydroxycarboxamido-(4S)-methyl-carboxamyl-aminopyrrolidine:The starting ester 57a (351 mg, 0.95 mmole) is converted to the relativehydroxamic acid as described for compound 48b and eluted through flashsilica with EtOAc:MeOH (8:1) to give the title compound as a white solidwhich was recrystallized from acetonitrile:water to give whitecrystalls. ESI MS: m/z (rel intensity) 41 1.0 (M⁺+K, 30), 373.1 (M⁺+H,100), 316 (32).

Example 58

[0325] a.(1N)-4-Methoxyphenylsulfonyl-(2R)-carbomethoxy-(4S)-N-1-oxo-2R-benzyloxypropyl)-aminopyrrolidine:The starting amine 42b (465 mg, 1.48 mmole), and the startingL-o-benzyllactic acid (319 mg, 1.78 mmole) is taken in 4 mL of DMF inthe presence of 1.5 mL of N-methylmorpholine, EDAC (568 mg, 2.96 mmole)and HOBT (599 mg, 4.44 mmole). The resulting mixture is stirred at rtfor 16 hr and then partitioned between 1N HCl and EtOAc. The organiclayer is then washed 1× with dil NaHCO₃, 1× with brine, dried overMgSO₄, filtered and evaparated. The crude residue is thenchromatographed with hexane:EtOAc (2:1 to 1:3) to give the titlecompound. ESI MS: m/z (rel intensity) 477.2 (M⁺+H, 100), 494.2 (M⁺+NH₃,10).

[0326] b.(1N)-4-Methoxyphenylsulfonyl-(2R)-N-hydroxycarboxamido-(4S)-N-(1-oxo-2R-benzyloxypropyl)aminopyrrolidine:The starting methylester 58b (480 mg, 1.01 mmole) is taken in 2 mL ofmethanol treated with NH₂OK (2.5 mL, 1.25 M in methanol, solutionprepared as described in Fieser and Fieser, Vol 1, p 478) and stirredovernight. The following morning, dry silica (3 mL) is added to themixture and the solvent removed under vaccuum. The dry silica is pouredon the top of a flash silica gel column which is subsequently elutedwith EtOAc:MeOH (1:0Ø4:1) to give 338 mg (70%) of a white foamy solid.ESI MS: m/z (rel intensity) 478.3 (M⁺+H, 100), 500.2 (M⁺+Na, 12).

Example 59

[0327] a. 2R-benzyloxy-3-phenylproionic acid: Sodium hydride (2.9 g, 120mmole), is washed 2 times with hexane and covered with 50 mL of DMF. Thestarting L-3-phenyllactic acid (5 g, 30.1 mmole) is then added inportions and, after fizzing ceased, the mixture is heated to 55° C. for1 hr. The mixture is then cooled to 0° C. and benzyl bromide (4.3 mL,36.1 mmole) is added dropwise. The mixture is heated to 60° C. for 3 hrand then partitioned between hexane:EtOAc (1:1) and 1N HCl. The organiclayer is washed with brine, dried over MgSO₄, filtered and evaparated.The residue is chromatographed over flash silica with hexane:EtOAc (9:1to 0:1) to give a colorless oil. ESI MS: m/z (rel intensity) 274.3(M⁺+NH₃, 100).

[0328] b.(1N)-4-Methoxyphenylsulfonyl-(2R)-carbomethoxy-(4S)-N-(1-oxo-2R-benzyloxy-3-phenylpropyl)-aminopyrrolidine:The starting amine 44a (800 mg, 2.55 mmole), and the starting benzyllactic acid 59a (784 mg, 3.06 mmole) is taken in 5 mL of DMF in thepresence of 1 mL of N-methylmorpholine, EDAC (979 mg, 5.10 mmole) andHOBT (1.03 mg, 7.65 mmole). The resulting mixture is stirred at rt for16 hr and then partitioned between 1N HCl and EtOAc. The organic layeris then washed 1× with dil NaHCO₃, 1× with brine, dried over MgSO₄,filtered and evaparated. The crude residue is then chromatographed withhexane:EtOAc (8:1 to 1:1) to give the title compound. ESI MS: m/z (relintensity) 553.2 (M⁺+H 100), 570.3 (M⁺+NH₃, 18).

[0329] c.(1N)-4-Methoxyphenylsulfonyl-(2R)-N-hydroxycarboxamido-(4S)-N-(1-oxo-2R-benzyloxy-3-phenylpropyl)-aminopyrrolidine:The staring methylester 59b (700 mg, 1.27 mmole) is taken in 2 mL ofmethanol, treated with NH₂OK (2.5 mL, 1.25 M in methanol, solutionprepared as described in Fieser and Fieser, Vol 1, p 478) and stirredovernight. The following morning, dry silica (3 mL) is added to themixture and the solvent removed under vaccuum. The dry silica is pouredon the top of a flash silica gel column which is subsequently elutedwith hexane:EtOAc (1:1Ø0:1) to give a white foamy solid. ESI MS: m/z(rel intensity) 553.3 (M⁺+H, 100), 576.3 (M⁺+Na, 23).

Example 60

[0330] a.(1N)-4-Methoxyphenylsulfonyl-(2R)-carbomethoxy-(4S)-N-(1-oxo-2R-benzyloxy-propyl)propyl-aminopyrrolidine:The starting amine 44a (636 mg, 1.79 mmole), and the startingL-o-benzyllactic acid (390 mg, 2.15 mmole) is taken in 5mL of DMF in thepresence of 1 mL of N-methylmorpholine, EDAC (687 mg, 3.58 mmole) andHOBT (762 mg, 5.37 mmole). The resulting mixture is stirred at rt for 16hr and then partitioned between 1N HCl and EtOAc. The organic layer isthen washed 1× with dil NaHCO₃ , 1× with brine, dried over MgSO ₄,filtered and evaparated. The crude residue is then chromatographed withhexane:EtOAc (8:1 to 1:1) to give the title compound. ESI MS: m/z (relintensity) 595.2 (M⁺+H, 100).

[0331] b.(1N)-4-Methoxyphenylsulfonyl-(2R)-carbomethoxy-(4S)-N-(1-oxo-2R-hydroxy-propyl)-propyl-aminopyrrolidine:The starting ether 60a (700 mg, 1.35 mmole) is taken in 25 mL ofmethanol with catalytic 10% Pd-C and H₂SO₄ and hydrogenated for 3 hrs at54 psi in a Parr apparatus. The material is then filtered through a padof celite, evaparated to dryness and chromatographed over flash silicato give a clear gum. ESI MS: m/z (rel intensity) 429.3 (M⁺+H, 100),446.3 (M+NH₃, 12).

[0332] c.(1N)-4-Methoxypbenylsulfonyl-(2R)-carbomethoxy-(4S)-N-(1-oxo-2R-hydroxypropyl)-propyl-aminopyrrolidine:The starting methylester 60b (331 mg, 0.771 mmole) is taken in 1 mL ofmethanol, treated with NH₂OK (1.23 mL, 1.25 M in methanol, solutionprepared as described in Fieser and Fieser, Vol 1, p 478) and stirredovernight. The following morning, dry silica (3 mL) is added to themixture and the solvent removed under vaccuum. The dry silica is pouredon the top of a flash silica gel column which is subsequently elutedwith hexane:EtOAc (1:1Ø0:1) to give a white foamy solid. ESI MS: m/z(rel intensity) 519.3 (M⁺+H, 100), 536.3 (M⁺+NH₃, 60).

Example 61

[0333] a.(1N)-4-Methoxyphenylsulfonyl-(2R)-carbomethoxy-(4S)-N,N-(1-oxo-2R-benzyloxy-3-phenylpropyl)-propyl-aminopyrrolidine:The acid 59a (530 mg, 1.68 mmole) was taken in 15 mL of CH₂Cl₂ andtreated with oxalyl chloride (293 mL, 3.37 mmole). A catalytic drop ofDMF was added and the mixture was stirred for a total of 3.5 hrs andthen evaparated to dryness. The residue was taken in 15 mL of CH₂Cl₂ andadded to a solution of the starting amine 44a (449 mL, 1.26 mmole) in 10mL of CH₂Cl₂ and 2 mL of triethyl amine. The resulting solution wasstirred for 16 hrs. and then partitioned between EtOAc and 1 N HCl. Theorganic layer was washed 1 time with 1 N HCl, 2 times with NaHCO₃, 1time with brine, dried over MgSO₄, filtered and evaporated to give 740mg of crude gum. This is then chromatographed over flash silica withhexane:EtOAc (4:1 to 1:2) to give a pale yellow gum. ESI MS: m/z (relintensity) 595.2 (M⁺+H, 100)

[0334] b.(1N)-4-Methoxyphenylsulfonyl-(2R)-carbomethoxy-(4S)-N-(1-oxo-2R-hydroxy-3-phenylpropyl)-propyl-aminopyrrolidine:The starting ether 61a (480 mg, 0.807 mmole) is taken in 20 mL ofmethanol with catalytic 10% Pd-C and H₂SO₄ and hydrogenated for 16 hrsat 50 psi in a Parr apparatus. The material is then filtered through apad of celite, evaparated to dryness and chromatographed over flashsilica with EtOAc to give a clear gum. ESI MS: m/z (rel intensity) 505.3(M⁺+H, 100), 522.3 (M⁺+NH₃, 15).

[0335] c.(1N)-4-Methoxyphenylsulfonyl-(2R)-N-hydroxycarboxamido-(4S)-N-(1-oxo-2R-hydroxy-3-phenylpropyl)-propyl-aminopyrrolidine:The starting methylester 61b (307 mg, 0.608 mmole) is taken in 1 mL ofmethanol, treated with NH₂OK (1.23 mL, 1.25 M in methanol, solutionprepared as described in Fieser and Fieser, Vol 1, p 478) and stirredovernight. The following morning, dry silica (3 mL) is added to themixture and the solvent removed under vaccuum. The dry silica is pouredon the top of a flash silica gel column which is subsequently elutedwith hexane:EtOAc (1:1Ø0:1) to give a white foamy solid. ESI MS: m/z(rel intensity) 506.3 (M⁺+H, 100), 526.3 (M⁺+Na, 12).

Examples 62-63

[0336] In the following examples W and Z are hydrogen, and Y is OH, n is1, Ar is substituted or unsubstituted phenyl, and X and Q refer tosubstituents on the phenyl ring:

Example X Y 62 OMe CH₂ 63 OnBu CH₂ 64 OMe O 65 OnBu O 66 OMe SO₂ 67 OnBuSO₂

Example 62

[0337] a.(1N)-4-Methoxyphenylsulfonyl-(2R)-carbomethoxy-(4S)-1-piperidyl-pyrrolidine:The starting amine 42b (1.00 g, 3.19 mmole) is dissolved in 10 mL ofmethanol and stirred for 16 hrs in the presence of glutonic dialdehyde(961 mg, 50 wt % in water, 4.8 mmole), sodium cyanoborohydride (503 mg,8 mmole), sodium acetate (1 g) and 1 mL of acetic acid. The mixture isevaparated to dryness and then partitioned between dil. NaHCO₃ and EtOAcand the organic layer is washed 2 times with NaHCO₃, 1 time with brine,dried over MgSO₄, filtered and evaparated to give a clear colorlessglass which is chromatographed over flash silica with hexane:EtOAc (4:1to 1:1) to give the desired product as a glear glass. ESI MS: m/z (relintensity) 383 (M⁺+H, 100), 211 (38).

[0338] b.(1N)-4-Methoxyphenylsulfonyl-(2R)-N-hydroxycarboxamido-(4S)-1-piperidyl-pyrrolidine:The starting methylester 62a (1.00 g, 2.62 mmole) is taken in 3 mL ofmethanol, treated with NH₂OK (4 mL, 1.25 M in methanol, solutionprepared as described in Fieser and Fieser, Vol 1, p 478) and stirredovernight. The following morning, dry silica (4 mL) is added to themixture and the solvent removed under vaccuum. The dry silica is pouredon the top of a flash silica gel column which is subsequently elutedwith EtOAc:MeOH (1:0Ø4:1) to give a pale orange solid. ESI MS: m/z (relintensity) 384 (M⁺+H, 100), 406 (M⁺+Na, 82), 422 (M⁺+K, 65).

Example 63

[0339] a.(1N)-4-n-Butoxyphenylsulfonyl-(2R)-carbomethoxy-(4S)-1-piperidyl-pyrrolidine:The starting amine 43c (1.06 g, 1.88 mmole) is taken in 10 mL of DMF and1.5 mL of NEt₃ and treated with 2 mL of 2-bromoethyl ether. Theresulting mixture is then heated to 60° C. for 16 hr and partitionedbetween dil Na₂CO₃ and EtOAc. The organic layer is then dried overMgSO₄, filtered and evaparated. The crude residue was chromatographedover flash silica with Hexane:EtoAc (1:1 to 0:1) to give the titlecompound as a clear oil. ESI MS: m/z (rel intensity) 425 (M⁺+H).

[0340] b.(1N)-4-n-Butoxyphenylsulfonyl-(2R)-N-hydroxycarboxamido-(4S)-1-piperidyl-pyrrolidine:The starting methylester 63a (851 mg, 2.01 mmole) is taken in 1 mL ofmethanol, treated with NH₂OK (0.381 mL, 0.86 M in methanol, solutionprepared as described in Fieser and Fieser, Vol 1, p 478) and stirredovernight. The following morning, dry silica (2 mL) is added to themixture and the solvent removed under vaccuum. The dry silica is pouredon the top of a flash silica gel column which is subsequently elutedwith EtOAc:MeOH (1:0Ø9:1) to give 543 mg (64%) of a pale orange solid.This was recrystallized from hexane:EtOAc to give pale orange solid. ESIMS: m/z (rel intensity) 426.1 (M⁺+H).

Example 64

[0341] a.(1N)-4-Methoxyphenylsulfonyl-(2R)-carbomethoxy-(4S)-morpholinopyrrolidine:The starting amine 42b (590 mg, 1.88 mmole) is taken in 4 mL of DMF and1 mL of NEt₃ and treated with 1 mL of 2-bromoethyl ether. The resultingmixture is then heated to 60° C. for 3 hr and partitioned between dilNaCO₃ and EtOAc. The organic layer is then dried over MgSO₄, filteredand evaparated. The crude residue was chromatographed over flash silicawith EtOAc:MeOH (9:1) to give the title compound as a white solid. ESIMS: m/z (rel intensity) 385.1 (M⁺+H).

[0342] b.(1N)-4-Methoxyphenylsulfonyl-(2R)-N-hydroxycarboxamido-(4S)-morpholinopyrrolidine:The starting methylester 64a (310 mg, 0.86 mmole) is treated with NH₂OK(2 mL, 1.25 M in methalol) in 4 mL mL of methanol as described for 63bto give material which is puffed to a white solid under vacuum and notrecrystallized. ESI MS: m/z (rel intensity) 386.1 (M⁺+H, 100), 565.1(12), 424.0 (15), 408.1 (M+NH₄ ⁺, 7), 218.1 (20), 202.1 (13).

Example 65

[0343] a.(1N)-4-n-Butoxyphenylsulfonyl-(2R)-carbomethoxy-(4S)-morpholinopyrrolidine:The starting amine 43c (7.2 g, 20.2 mmole), was taken in 50 mL of DMFand 15 mL of Et₃N with 2-bromoethyl ether and converted to the titlecompound as described for compound 63a. ESI MS: m/z (rel intensity)427.18 (M⁺+H).

[0344] b.(1N)-4-n-Butoxyphenylsulfonyl-(2R)-N-hydroxycarboxamido-(4S)-morpholinopyrrolidine:The starting methylester 65a (6.5 g, 15.2 mmole) is treated with NH₂OK(24 mL, 1.25 M in methalol) in 20 mL mL of methanol as described for 63bto give material which is puffed to a white solid under vacuum and notrecrystallized. ESI MS: m/z (rel intensity) 428.08 (M⁺+H, 100), 450.07(M⁺+Na, 8), 465.99 (M⁺+K, 15).

Example 66

[0345] a.(1N)-4-Methoxyphenylsulfonyl-(2R)-carbomethoxy-(4S)-(4,4-dioxythio-morpholino)-pyrrolidine:The starting amine 42b (560 mg, 1.79 mmole), was taken in 10 mL of DMFand 1 mL of N-methylmorpholine with di-2-bromoethylsulfone (500 mg, 1.79mmole) and converted to the title compound as described for compound63a. ESI MS: m/z (rel intensity) 433.1 (M⁺+H).

[0346] b.(1N)-4-Methoxyphenylsulfonyl-(2R)-N-hydroxycarboxamido-(4S)-4,4-dioxythiomorpholino)-pyrrolidine:The starting methyl ester 66a (420 mg, 976 mmole) was converted to thetitle compound as described for compound 63b. This material was thenrecrystallized from EtOAC methanol to give first crop crystalls andsecond crop crystalls. ESI MS: m/z (rel intensity) 434.0 (M⁺+H, 100),456.0 (M⁺+Na, 32).

Example 67

[0347] a.(1N)-4-n-Butoxyphenylsulfonyl-(2R)-carbomethoxy-(4S)-(4,4-dioxythio-morpholino)-pyrrolidine:The starting amine 43c (1.00 g, 2.81 mmole), was taken in 5 mL of DMFand 2 mL of N-methylmorpholine with di-2-bromoethylsulfone (750 mg, 2.68mmole) and converted to the title compound as described for compound63a. ESI MS: m/z (rel intensity) 475.0 (M⁺+H)

[0348] b.(1N)-4-n-Butoxyphenylsulfonyl-(2R)-N-hydroxycarboxamido-4S)-4,4-dioxythiomorpholino)-pyrrolidine:The starting methylester 67a (1.01 g, 2.83 mmole) is treated with NH₂OK(4 mL, 1.25 M in methalol) in 4 mL of methanol as described for 63b togive material which is puffed to a white solid under vacuum and notrecrystallized. ESI MS: m/z (rel intensity) 476.1 (M⁺+H, 100), 498.1(M⁺+Na, 22).

Example X Q R P 68 OMe Me H H 69 OnBu Me H H 70 OMe CH₂CH═CH₃ H H 71OnBu H CH₃ CH₃ 72 OnBu H H CH₃ 73 O(CH₂)₂OMe CH₃ H H 74 OPh CH₃ H H 75OCH(CH₃)₂ CH₃ H H

Example 68

[0349] a.(1N)-4-Methoxyphenylsulfonyl-(2R)-carbomethoxy-4S)-1N-3N-methylhydantoyl)-pyrrolidine:Diethylazodicarboxylate (1.8 mL, 11.42 mmole) is added to a stirredsolution of the starting alcohol 1a (3.0 g, 9.51 mmole),triphenylphosphene (3.74 g, 9.51 mmole), and 1-methylhydantoin (1.3 g,11.42 mmole) in 30 mL of CH₂Cl₂ and stirred for 16 hrs at rt. Themixture is then chromatographed over flash silica with hexane and thenhexane:EtOAc (1:1) to give colorless glass which is recrystallized frommethanol to give a white powder. ESI MS: m/z (rel intensity) 412.1(M⁺+H, 100), 429.1 (M⁺+NH₃, 45).

[0350] b.(1N)-4-Methoxyphenylsulfonyl-(2R)-N-hydroxycarboxamido-(4S)-1N-(3N-methylhydantoyl)-pyrrolidine:The starting methyl ester 68a (500 mg, 1.22 mmole) is taken in 7 mL ofmethanol/tetrahydrofuran (1:1), and treated with NH₂OK (2.5 ml, 1.25M inmethanol) and stirred overnight. The following morning, dry silica (1.5mL) is added to the mixture and the solvent removed under vacuum. Thedry silica is poured on top of a flash silica column which issubsequently eluted with ethyl acetate followed with ethyl acetate (9:1)to give a clear glass which is puffed to a foamy solid by slight heatingunder vacuum. The product is recrystallized from cold methanol to give awhite powder. ESI MS: m/z (rel intensity) 413.0 (M⁺+H, 100), 430.0(M⁺+NH₃, 55).

Example 69

[0351] a.(1N)-4-n-Butoxyphenylsulfonyl-(2R)-carbomethoxy-(4S)-1-(3N-methyl-hydantoyl)-pyrrolidine:Diethylazodicarboxylate (1.6 mL, 10.24 mmole) is added to a stirredsolution of the starting alcohol 29a (3.05 g, 8.53 mmole),triphenylphosphine (3.36 g, 12.80 mmole), and 1-methyl-hydantoin (1.17mg, 10.24 mmole) in 60 mL of CH₂Cl₂ and stirred for 16 hrs at rt. Themixture is then chromatographed over silica with hexane followed byhexane:EtOAc (1:1) and finally with EtOAc to give a colorless gum. Theproduct was recrystallized from EtOAc-hexane to give a white powder. ESIMS: m/z (rel intensity) 454.05 (M⁺+H, 100), 471.05 (M⁺+NH₃, 30).

[0352] b.(1N)-4-n-butoxyphenylsulfonyl-(2R)-N-hydroxycarboxamido-(4S)-1N-(3N-methylhydantoyl)-pyrrolidine:The starting methyl ester 69a (500 mg, 1.22 mmole) is taken in 7 mL ofmethanol/tetrahydrofuran (1:1), and treated with NH₂OK (2.5 ml, 1.25 Min methanol) and stirred overnight. The following morning, dry silica(1.5 ml) is added to the mixture and the solvent removed under vacuum.The dry silica is poured on top of a flash silica column which issubsequently eluted with ethyl acetate followed with ethylacetate:methanol (9:1) to give a clear glass which is puffed to a foamysolid by slight heating under vacuum. The product is recrystallized fromcold methanol to give a white powder. ESI MS: m/z (rel intensity) 455.0(M⁺+H, 100), 472.0 (M⁺+NH₃, 50).

Example 70

[0353] a.(1N)-4-n-butoxyphenylsulfonyl-(2R)-carbomethoxy-(4S)-1N-(3N-allylhydantoyl)-pyrrolidine:Diethylazodicarboxylate (1.1 mL, 6.98 mmole) is added to a stirredsolution of the starting alcohol 1a (2.08 g, 5.82 mmole),triphenylphosphine (2.29 g, 8.73 mmole), and 1-allylhydantoin (979 mg,6.98 mmole) in 40 mL of CH₂Cl₂ and stirred for 16 hrs at rt. The mixtureis then chromatographed over silica with hexane:EtOAc (8:2) followed byhexane:EtOAc (1:1) to give a colorless gum. ESI MS: m/z (rel intensity)480.0 (M⁺+H, 100), 497.0 (M⁺+NH₃, 20).

[0354] b.(1N)-4-n-butoxyphenylsulfonyl-(2R)-N-hydroxycarboxamido-(4S)-1N-(3N-allylhydantoyl)-pyrrolidine:The starting methyl ester 70a (549 mg, 1.15 mmole) is taken in 2 mL ofmethanol/tetrahydrofuran (1:1), and treated with NH₂OK (1.5 mL, 1.25 Min methanol) and stirred overnight. The following morning, dry silica(1.5 mL) is added to the mixture and the solvent removed under vacuum.The dry silica is poured on top of a flash silica column which issubsequently eluted with ethyl acetate followed with ethylacetate:methanol (8:2) to give a clear glass which is purified to afoamy solid by slight heating under vacuum. The product wasrecrystallized from cold methanol to give a of white powder. ESI MS: m/z(rel intensity) 481.2 (M⁺+H, 100), 498.2(M⁺+NH₃, 60).

Example 71

[0355] a.(1N)-4-n-butoxyphenylsulfonyl-(2R)-carbomethoxy-(4S)-1N-(4-dimethylhydantoyl)pyrrolidine:Diethylazodicarboxylate (0.530 mL, 3.36 mmole) is added to a stirredsolution of the starting alcohol 29a (1.00 g, 2.80 mmole),triphenylphosphine (1.10 g, 4.20 mmole), and 5,5-dimethylhydantoin (430mg, 3.36 mmole) in 20 mL of CH₂Cl₂ and stirred for 16 hrs at rt. Themixture is then chromatographed over silica with hexane:EtOAc (8:2)followed by hexane:EtOAc (1:1) to give a colorless gum. ESI MS: m/z (relintensity) 468.1 (M⁺+H, 100), 485.1 (M⁺+NH₃, 30).

[0356] b.(1N)-4-n-butoxyphenylsulfonyl-(2R)-N-hydroxycarboxamido-(4S)-1N-(4-dimethylhydantoyl)-pyrrolidine:The starting methyl ester 71a (754 mg, 1.61 mmole) is taken in 2 mL ofmethanol/tetrahydrofuran (1:1), and treated with NH₂OK (2.0 mL, 1.25M inmethanol) and stirred overnight. The following morning, dry silica (1.5mL) is added to the mixture and the solvent removed under vacuum. Thedry silica is poured on top of a flash silica column which issubsequently eluted with hexane:ethyl acetate (1:1) followed withhexane:ethyl acetate (2:8) and finally with ethyl acetate:methanol (8:2)to give a clear glass which is puffed to a foamy solid by slight heatingunder vacuum. The product is recrystallized from cold methanol to givethe title compound as a white powder. ESI MS: m/z (rel intensity) 469.0(M⁺+H, 100), 486.0 (M⁺+NH₃, 10).

Example 72

[0357] a.(1N)-4-n-butoxyphenylsulfonyl-(2R)-carbomethoxy-(4S)-1N-(4S-methyl-hydantoyl)-pyrrolidine:Diethylazodicarboxylate (0.530 mL, 3.36 mmole) is added to a stirredsolution of the starting alcohol 29a (1.00 g, 2.80 mmole),triphenylphosphine (1.10 g, 4.20 mmole), and (L)-5-methylhydantoin (383mg, 3.36 mmole) in 20 mL of CH₂Cl₂ and stirred for 16 hrs at rt. Themixture is then chromatographed over silica with hexane:EtOAc (8:2)followed by hexane:EtOAc (1:1) to give a colorless gum. This is thenrepurified over a second column eluting first with hexane:EtAcO (1:1)followed by EtOAc:hexane (8:2). 1H NMR showes a mitsunobu impurity (20%)remaining after two column purifications and the material is carriedforwared to the next step without further purification. ESI MS: m/z (relintensity) 454.0 (M⁺+H, 100), 471.0 (M⁺+NH₃, 20).

[0358] b.(1N)-4-n-butoxyphenylsulfonyl-(2R)-N-hydroxycarboxamido-(4S)-1N-(4S-methylhydantoyl)-pyrrolidine:The starting methyl ester 72a (497 mg, 1.10 mmole) is taken in 2 mL ofmethanol/tetrahydrofuran (1:1), and treated with NH₂OK (1.5 mL, 1.25M inmethanol) and stirred overnight. The following morning, dry silica (1.5mL) is added to the mixture and the solvent removed under vacuum. Thedry silica is poured on top of a flash silica column which issubsequently eluted with ethyl acetate followed with ethylacetate:methanol (9:1) to give a clear glass which is puffed to a foamysolid by slight heating under vacuum. The product is recrystallized fromcold methanol to give the title compound as a of white powder. ESI MS:m/z (rel intensity) 455.0 (W+H, 100), 472.0 (M⁺+NH₃, 30).

Example 73

[0359] a.(1N)-4-(2-methoxyethoxy)-phenylsulfonyl-(2R)-carbomethoxy-(4S)-1N-(3N-methylhydantoyl)-pyrrolidine:Diethylazodicarboxylate (0.546 mL, 3.47 mmole) is added to a stirredsolution of the starting alcohol 34b (1.04 g, 2.89 mmole),triphenylphosphine (1.14 g, 4.34 mmole), and 1-methylhydantoin (396 mg,3.47 mmole) in 20 mL of CH₂Cl₂ and stirred for 16 hrs at rt. The mixtureis then chromatographed over silica with hexane:EtOAc (1:1) followed byhexane: EtOAc (2:8) to give a colorless gum. ESI MS: m/z (rel intensity)456.14 (M⁺+H, 100), 473.15 (M⁺+NH₃, 10).

[0360] b.(1N)-4-(2-methoxyethoxy)-phenylsulfonyl-(2R)-N-hydroxycarboxamido-(4S)-1N-(3N-methylhydantoyl)-pyrrolidine:The starting methyl ester 73a (725 mg, 1.59 mmole) is taken in 2 mL ofmethanol/tetrahydrofuran (1:1), and treated with NH₂OK (2 mL, 1.25 M inmethanol) and stirred overnight. The following morning, dry silica (1.5mL) is added to the mixture and the solvent removed under vacuum. Thedry silica is poured on top of a flash silica column which issubsequently eluted with ethyl acetate followed with ethylacetate:methanol (8:2) to give a clear glass which is purffed to a foamysolid by slight heating under vacuum. The product was recrystallizedfrom cold methanol to give the title compound as a white powder. ESI MS:m/z (rel intensity) 457.08 (M⁺+H, 100), 474.09 (M⁺+NH₃, 60).

Example 74

[0361] a.(1N)-4-phenoxyphenylsulfonyl-(2R)-carbomethoxy-(4S)-1N-(3N-methylhydantoyl)-pyrrolidine:Diethylazodicarboxylate (0.570 mL, 3.62 mmole) is added to a stirredsolution of the starting alcohol 35b (1.14 g, 3.02 mmole),triphenylphosphine (1.19 g, 4.53 mmole), and 1-methyl-hydantoin (413 mg,3.62 mmole) in 20 mL of CH₂Cl₂ and stirred for 16 hrs at rt. The mixtureis then chromatographed over silica with hexane:EtOAc (8:2) followed byhexane:EtOAc (1:1) with product eluting with Hexane:EtOAc (2:8) to givea colorless gum. ESI MS: m/z (rel intensity) 474.03 (M⁺+H, 100), 491.03(M⁺+NH₃, 20).

[0362] b.(1N)-4-phenoxyphenylsulfonyl-(2R)-N-hydroxycarboxamido-(4S)-1N-(3N-methylhydantoyl)-pyrrolidine:The starting methyl ester (1.50 g, 1.59 mmole) is taken in 10 mL ofmethanol/tetrahydrofuran (1:1), and treated with NH₂OK (5 mL, 1.25M inmethanol) and stirred overnight. The following morning, dry silica (5mL) is added to the mixture and the solvent removed under vacuum. Thedry silica is poured on top of a flash silica column which issubsequently eluted with ethyl acetate:hexane (1:1), then ethyl acetatefollowed with ethyl acetate:methanol (8:2) to give a clear glass whichis puffed to a foamy solid by slight heating under vacuum. The productis recrystallized from cold methanol to give the title compound as awhite powder. ESI MS: m/z (rel intensity) 475.09 (M⁺+H, 100), 497.07(M⁺+NH₃, 60).

Example 75

[0363] a.(±)-(1N)-4-Methoxyphenylsulfonyl-(2R)-carbomethoxy-5-pyrrolidinone: The2-carboxy-β-lactam starting material (10 g, 77.5 mmoles) is dissolved in200 ml of methanol at 0° C. followed by the addition of 0.76Mdiazomethane until the color of the reaction mixture remained yellow.The reaction is then stirred for an additional 30 minutes. This isevaporated down to get rid of excess methanol and diazomethane. Theyield is quantitative and the product is carried forward without furtherpurification.

[0364] The methyl ester produced above (11.08 g, 77.5 mmoles) isdissolved in 500 mL of dry THF at 0° C. followed by the one portionaddition of t-butoxide (9.15 g, 77.5 mmoles) and stirred for 1 hour.Next, 4-methoxybenzene sulfonyl chloride (19.2 g, 93.0 mmoles) is addedand this stirred over night. The reaction is quenched with saturatedsodium bicarbonate until basic and extracted with ether 3 times. Theether layer is washed with 1N HCl, sodium bicarbonate, and ammoniumchloride, dried over magnesium sulfate and evapoated done.Chromotography is performed on silica gel using a solvent system ofethyl acetate:hexane (1:1) to give the title compound. Cl⁺ MS: m/z (relintensity) 314.0 (M⁺+H, 100).

[0365] b.(±)-(1N)-4-Methoxyphenylsulfonyl-(2R)-carboxyl-5-pyrrolidinone: Thesulfonated methyl ester 75a (8.5 g, 27.12 mmoles) is dissolved in 60 mLof a THF and methanol (3:1). Lithium hydroxide (2.27 g, 94.9 mmoles) isthen added in THF and methanol (3:1). An additional 10 ml of methanol isadded to the reaction mixture to improve solubility. The reactionstirred for 3 hours. The reaction is quenched with water and thenevaporated down to get rid of the organic solvents. The water layer isextracted one time with ether. Then the water layer is acidified to pH=2and this is extracted with ethyl acetate 3 times and washed with sodiumchloride and dried over magnesium sulfate. This is evaporated down togive give the title compound. Cl⁺ MS: m/z (rel intensity) 300.0 (M⁺+H,100).

[0366] c.(±)-(1N)-4-Methoxyphenylsulfonyl-(2R)-O-benzyl-N-hydroxycarboxamido-5-pyrrolidinone:

[0367] The carboxylic acid 75b (1.0 g, 3.3 mmoles) is dissolved in 15 mLof DMF at 0° C. followed by the addition of triethyl amine (1.37 mL, 9.9mmoles), 4-methylmorpholine N-oxide (1.08 g, 9.9 mmoles),1-hydroxy-benzotriazole (1.33 g, 9.9 mmoles), and1-ethyl-3(3-dimethyl-aminopropyl)carbodiimide (0.76 g, 4.01 mmoles).This stirred for 30 minutes followed by the addition of the benzylamine(0.64 g, 4.01 mmoles). The reaction stirred overnight. The reaction isquencehed with saturated sodium bicarbonate and then extracted withethyl acetate 3 times, washed with 1NHCl and sodium chloride, dried overmagnesium sulfate and evaporated down. Chromotography is run on silicagel using ethyl acetate and methylene chloride (5:1) to give give thetitle compound. Cl⁺ MS: m/z (rel intensity) 404.0 (M⁺+H, 100).

[0368] d.(±)-(1N)-4-Methoxyphenylsulfonyl-(2R)-N-hydroxycarboxamido-5-pyrrolidinone:The benzyl protected lactam 75c (0.42 g, 1.04 mmoles) is dissolved in 20mL of ethyl acetate followed by the addition of palladium on activatedcarbon (wet) (0.042 g, [10% of weight]). The reaction flask is degassedof all oxygen and then put under hydogen balloon pressure for overnight.After the flask is degassed of hydrogen, the palladium is filtered offthrough cellite and the ethyl acetate is rotovapped off. The compound isrecrystallized with ethyl acetate and hexane to give the title compound.ESI MS: m/z (rel intensity) 314.0 (M⁺+H, 100).

Example 76

[0369] a.(1N)-Methoxyphenylsulfonyl-(2R)-carbomethoxy-4,4-dithiolethyl-pyrrolidine:The ketone 25a (1.5 g, 4.79 mmol) is dissolved in 30 mL of anhydrousdichloromethane and then ethanethiol (0.53 mL, 7.18 mmol) and boranetrifluoride etherate (0.24 mL, 1.91 mmol) is added. The resultingmixture is stirred at room temperature for 14 h. The reaction mixture isquenched by the the addition of 1N sodium hydroxide and then extracted 3times with ethyl acetate. The organic layers are washed with water andsaturated ammonium chloride solution, dried (MgSO₄), filtered andconcentrated under reduced pressure to give the title compound. Cl⁺ MS:m/z 420 (M⁺+H)).

[0370] b.(1N)-4-Methoxyphenylsulfonyl-(2R)-N-hydroxycarboxamido-4,4-dithiolethylpyrrolidine:The thioketal 76a (0.32 g, 0.89 mmol) is added to a 1.5 M solution ofpotassium hydroxylamine solution (4.0 mL, prepared as described inFieser and Fieser, Vol. 1, p. 478.). The reaction mixture is stirredovernight and then acidified with 1N HCl. The resulting mixture is thenextracted 3 times with ethyl acetate, dried (MgSO₄), filtered andconcentrated under reduced pressure. Chromotography was performed onsilica gel using EtOAc:hexane:formic acid (1:1:0.1) as the eluent togive the title compound. ESI MS: m/z 421 (M⁺+H), 443 (M⁺+Na).

Examples 77-180

[0371] The following compounds are made using the methods described andexemplified above. In these Examples R₁ is HONH, Z and W are hydrogen,and Y and Ar substitution, as well as ring size are described in thechart below. Hence, a simplified diagram of the molecule exemplified is:

Y Ar n Example 78 —OH 4-NO₂—C₆H₄— 1 Example 79 —OH 4-i-BuO—C₆H₄— 1Example 80 —OH 4-(C₆H₅)O—C₆H₄— 1 Example 81 —OH 4-(4-F—C₆H₄)O—C₆H₄— 1Example 82 —OH 4-(4-Cl—C₆H₄)O—C₆H₄— 1 Example 83 —OH4-(4-Br—C₆H₄)O—C₆H₄— 1 Example 84 —OH 4-(4-Me—C₆H₄)O—C₆H₄— 1 Example 85—OH 4-(4-MeO—C₆H₄)O—C₆H₄— 1 Example 86 —OH 4-(4-CN—C₆H₄)O—C₆H₄— 1Example 87 —OH 4-(4-Me₂N—C₆H₄)O—C₆H₄— 1 Example 88 —OH 4-EtO—C₆H₄— 1Example 89 —OH 4-i-PrO—C₆H₄— 1 Example 90 —OH 4-n-PrO—C₆H₄— 1 Example 91—OH 4-Br—C₆H₄— 1 Example 92 —OH 4-C₆H₅—C₆H₄— 1 Example 93 —OH4-(4-F—C₆H₅)—C₆H₄— 1 Example 94 —OH 4-(4-Cl—C₆H₅)—C₆H₄— 1 Example 95 —OH4-(4-Br—C₆H₅)—C₆H₄— 1 Example 96 —OH 4-(4-Me₂N—C₆H₄)—C₆H₄— 1 Example 97—OH 4-(4-CN—C₆H₄)—C₆H₄— 1 Example 98 —OH 4-(4-MeO—C₆H₄)—C₆H₄— 1 Example99 —OH 4-(4-C₅H₄N)O—C₆H₄— 1 Example 100 —OH 4-(3-C₅H₄N)O—C₆H₄— 1 Example101 —OH 4-(2-C₅H₄N)O—C₆H₄— 1 Example 102 —OH C₆H₅CH₂CH₂— 1 Example 103—OH C₆H₅CH₂— 1 Example 104 —OH (4-C₅H₄N)CH₂CH₂— 1 Example 105 —OH(2-C₅H₄N)CH₂CH₂— 1 Example 106 —OH 4-(C₆H₁₁)O—C₆H₄— 1 Example 107 —OH4-(C₅H₁₁)O—C₆H₄— 1 Example 108 —OH 4-(C₆H₁₃)O—C₆H₄— 1 Example 109 —OH4-(CH₃OCH₂CH₂)O—C₆H₄— 1 Example 110 —OH 5-(2-pyridinyl)-2-thienyl- 1Example 111 —OH 5-(3-isoxazolyl)-2-thienyl- 1 Example 112 —OH5-(2-(methylthio)pyrimidin-4- 1 yl)-2-thienyl- Example 113 —OH5-(3-(1-methyl-5- 1 (trifluoromethyl)pyrazolyl)-2-thienyl- Example 114—NHP(O)(CH₃)C₆H₅ CH₃CH₂CH₂OC₆H₄— 1 Example 115 —NHCOCH₂C₆H₅CH₃CH₂CH₂OC₆H₄— 1 Example 116 —NHCO(2-pyridyl) CH₃CH₂CH₂OC₆H₄— 1 Example117 —NHCOCH₂NMe₂ CH₃CH₂CH₂OC₆H₄— 1 Example 118—NHCO-2-(1-methyl)-imidazyl CH₃CH₂CH₂OC₆H₄— 1 Example 119 —NHSO₂CH₃4-(4-C₅H_(4)O—C) ₆H₄— 1 Example 120 —NHCOC₆H₅ CH₃CH₂CH₂OC₆H₄— 1 Example121 —NMe₂ CH₃CH₂CH₂OC₆H₄— 1 Example 122 —N(CH₂CH₃)₂ CH₃CH₂CH₂OC₆H₄— 1Example 123 —NMe₂ 4-(4-C₅H₄N)O—C₆H₄— 1 Example 124 —N(CH₂CH₃)₂4-(4-C₅H₄N)O—C₆H₄— 1 Example 125 —N(CH₂CH₃)SO₂CH₃ CH₃CH₂CH₂OC₆H₄— 1Example 126 —N(CH₂CH₃)COCH₃ CH₃CH₂CH₂OC₆H₄— 1 Example 127—N(CH₂CH₃)SO₂CH₃ 4-(4-C₅H₄N)O—C₆H₄— 1 Example 128 —N(CH₂CH₃)COCH₃4-(4-C₅H₄N)O—C₆H₄— 1 Example 129 —N(CH₃)CO(2-pyridyl) CH₃CH₂CH₂OC₆H₄— 1Example 130 —N(CH₃)CO(4-pyridyl) CH₃CH₂CH₂OC₆H₄— 1 Example 131—N(CH₃)COC₆H₅ CH₃CH₂CH₂OC₆H₄— 1 Example 132—N(CH₃)CO-1N-methylpiperazine CH₃CH₂CH₂OC₆H₄— 1 Example 133 —N(CH₃)COHCH₃CH₂CH₂OC₆H₄— 1 Example 134 —N(CH₃)COCH₂OCH₃ CH₃CH₂CH₂OC₆H₄— 1 Example135 —N(CH₃)COCH(CH₃)₂ CH₃CH₂CH₂OC₆H₄— 1 Example 136 —N(CH₃)CO(furanyl)CH₃CH₂CH₂OC₆H₄— 1 Example 137 —N(CH₃)CO(oxazolinyl) CH₃CH₂CH₂OC₆H₄— 1Example 138 —N(CH₃)COCH₂CN CH₃CH₂CH₂OC₆H₄— 1 Example 139—N(CH₃)CO(CH2)N(CH₃)₂ CH₃CH₂CH₂OC₆H₄— 1 Example 140—N(CH₃)SO₂-3-(1N-methylimidazyl) CH₃CH₂CH₂OC₆H₄— 1 Example 141—N(CH₃)SO₂CH(CH₃)₂ CH₃CH₂CH₂OC₆H₄— 1 Example 142 —CH₂NHSO₂CH₃ CH₃OC₆H₄—1 Example 143 —CH₂NH SO₂C₆H₅ CH₃OC₆H₄— 1 Example 144 —CH₂NHCOC₆H₅CH₃OC₆H₄— 1 Example 145 —CH₂NHCOCH₂CH₂CH₃ CH₃OC₆H₄— 1 Example 146—CH₂N(CH₃)COCH₃ CH₃OC₆H₄— 1 Example 147 —CH₂N(CH₃)SO₂C₆H₅OMe CH₃OC₆H₄— 1Example 148 —CH₂N(CH₂C₆H₅)SO₂CH₃ CH₃OC₆H₄— 1 Example 149 —OH CH₃OC₆H₄— 2Example 150 —S—C₆H₅ CH₃OC₆H₄— 2 Example 151 —(OMe)₂ CH₃OC₆H₄— 2 Example152 —OH BrC₆H₄— 2 Example 153 -3-methyl-1-hydantoyl- 4-EtO—C₆H₄— 1Example 154 -3-methyl-1-hydantoyl- 4-i-PrO—C₆H₄— 1 Example 155-3-methyl-1-hydantoyl- 5-(2-pyridinyl)-2-thienyl- 1 Example 156-3-methyl-1-hydantoyl- 4-Br—C₆H₄— 1 Example 157 -3-methyl-1-hydantoyl-2-Me-4-Br—C₆H₄— 1 Example 158 -3-methyl-1-hydantoyl- 4-(C₆H₅)O—C₆H₄— 1Example 159 -3-methyl-1-hydantoyl- 4-(4-F—C₆H₄)O—C₆H₄— 1 Example 160-3-methyl-1-hydantoyl- (4-C₅H₄N)CH₂CH₂— 1 Example 161-3-methyl-1-hydantoyl- 4-(4-C₅H₄N)O—C₆H₄— 1 Example 162 -1N-morpholino4-EtO—C₆H₄— 1 Example 163 -1N-morpholino 4-i-PrO—C₆H₄— 1 Example 164-1N-morpholino 5-(2-pyridinyl)-2-thienyl- 1 Example 165 -1N-morpholino4-Br—C₆H₄— 1 Example 166 -1N-morpholino 2-Me-4-Br—C₆H₄— 1 Example 167-1N-morpholino 4-(C₆H₅)O—C₆H₄— 1 Example 168 -1N-morpholino4-(4-F—C₆H₄)O—C₆H₄— 1 Example 169 -1N-morpholino (4-C₅H₄N)CH₂CH₂— 1Example 170 -1N-morpholino 4-(4-C₅H₄N)O—C₆H₄— 1 Example 171-1N-valerolactamyl- (4-C₅H₄N)OC₆H₄— 1 Example 172 -1N-valerolactamyl-4-n-BuOC₆H₄— 1 Example 173 —(OMe)₂ CH₃CH₂OC₆H₄— 1 Example 174 —(OMe)₂CH₃CN₂CH₂OC₆H₄— 1 Example 175 —(OMe)₂ 4-(4-C₅H₄N)O—C₆H₄— 1 Example 176—(OCH₂CH₃)₂ CH₃CH₂OC₆H₄— 1 Example 177 —(OCH₂CH₃)₂ CH₃CN₂CH₂OC₆H₄— 1Example 178 —(OCH₂CH₃)₂ 4-(4-C₅H₄N)O—C₆H₄— 1 Example 179 —(OCH₂CH₂OCH₃)CH₃CH₂OC₆H₄— 1 Example 180 —(OCH₂CH₂OCH₃) CH₃CN₂CH₂OC₆H₄— 1 Example 181—(OCH₂CH₂OCH₃) 4-(4-C₅H₄N)O—C₆H₄— 1

[0372] Examples 78-113 are prepared analogously to Example 1 using theappropriately functionalized sulfonyl chloride. The sulfonyl chlorideswhich are used to prepare the above examples are either purchased fromcommericial sources or prepared via known methods. For example, the4-phenoxyphenylsulfonyl chloride used for the preparation of Example 17,was prepared as described by R. J. Cremlyn et al in Aust. J. Chem. ,1979, 32, 445.52.

[0373] Examples 114-120 are prepared using methods described in examples42-61 using the appropriate alkyl, acyl, sulfonyl, phosphinyl orisocyanate derivative.

[0374] Examples 129-141 are prepared by first mono-methylating theappropriate primary amine derivative as described by S. Krishnamurthy etal in Tetrahedron Lett. 1983, 23 (33), 3315, and then adding adding theappropriate alkyl, acyl, sulfonyl, phosphinyl or isocyanate derivativeas described in examples 42-61.

[0375] Examples 142-148 are prepared from cyanide addition into mesylate15a followed by reduction to the corresponding free amine and treatmentwith the appropriate alkyl, acyl, sulfonyl, or phosphinyl derivative.

[0376] Examples 149-152 are prepared by ketalization or reduction and/ornucleophillic substitution of the appropriately functionalized4-ketopipecolic acid described by J. -P. Obrecht et al in OrganicSynthesis 1992, 200.

[0377] Examples 153-161 are prepared as described for example 68.

[0378] Examples 162-170 are prepared as described for example 65.

[0379] Examples 171-172 are prepared by acylation of a primary amine oftype 43c with 5-bromovaleryl chloride followed base promoted ringclosure and hydroxamic acid formation.

[0380] Examples 173-181 are prepared by standard ketalization methods ofketones of type 25a.

[0381] These examples provide the skilled artisan with sufficientguidance as to making the present invention and do not limit it in anyway.

[0382] Composition and Method of Use Examples

[0383] The compounds of the invention are useful to prepare compositionsfor the treatment of ailments and the like. The following compositionand method examples do not limit the invention, but provide guidance tothe skilled artisan to prepare and use the compounds, compositions andmethods of the invention. In each case the compounds formula I may besubstituted for the example compound shown below with similar results.

[0384] The methods of use exemplified do not limit the invention, butprovide guidance to the skilled artisan to use the compounds,compositions and methods of the invention. The skilled practitioner willappreciate that the examples provide guidance and may be varied based oncondition and the patient.

Example A

[0385] A tablet composition for oral administration, according to thepresent invention, is made comprising: Component Amount The compound ofExample 9 15. mg Lactose 120. mg Maize Starch 70. mg Talc 4. mgMagnesium Stearate 1. mg

[0386] (Other compounds having a structure according to Formula (I) areused with substantially similar results.)

[0387] A human female subject weighing 60 kg (132 lbs), suffering fromrheumatoid arthritis, is treated by a method of this invention.Specifically, for 2 years, a regimen of three tablets per day isadministered orally to said subject.

[0388] At the end of the treatment period, the patient is examined andis found to have reduced inflammation, and improved mobility withoutconcomitant pain.

Example B

[0389] A capsule for oral administration, according to the presentinvention, is made comprising: Component Amount (% w/w) The compound ofExample 3 15% Polyethylene glycol 85%

[0390] (Other compounds having a structure according to Formula (I) areused with substantially similar results.)

[0391] A human male subject weighing 90 kg (198 lbs), suffering fromosteoarthritis, is treated by a method of this invention. Specifically,for 5 years, a capsule containing 70 mg of the compound of Example 3 isadministered daily to said subject.

[0392] At the end of the treatment period, the patient is examined viaorthoscopy, and found to have no further advancement oferosion/fibrillation of the articular cartilage.

Example C

[0393] A saline-based composition for local administration, according tothe present invention, is made comprising: Component Amount (% w/w) Thecompound of Example 13  5% Polyvinyl alcohol 15% Saline 80%

[0394] (Other compounds having a structure according to Formula (I) areused with substantially similar results.)

[0395] A patient having deep corneal abrasion applies the drop to eacheye twice a day. Healing is speeded, with no visual sequelae.

Example D

[0396] A topical composition for local administration, according to thepresent invention, is made comprising: Component Composition (% w/v) Thecompound of Example 3 0.20 Benzalkonium chloride 0.02 Thimerosal 0.002d-Sorbitol 5.00 Glycine 0.35 Aromatics 0.075 Purified water q.s. Total =100.00

[0397] (Other compounds having a structure according to Formula (I) areused with substantially similar results.)

[0398] A patient suffering from chemical burns applies the compositionat each dressing change (b.i.d.). Scarring is substantially diminished.

Example E

[0399] A inhalation aerosol composition, according to the presentinvention, is made comprising: Component Composition (% w/v) Compound ofExample 2 5.0 Alcohol 33.0 Ascorbic acid 0.1 Menthol 0.1 SodiumSaccharin 0.2 Propellant (F12, F114) q.s. Total = 100.0

[0400] (Other compounds having a structure according to Formula (I) areused with substantially similar results.)

[0401] An asthma sufferer sprays 0.01 mL via a pump actuator into themouth while inhaling. Asthma symptoms are diminished.

Example F

[0402] A topical opthalmic composition, according to the presentinvention, is made comprising: Component Composition (% w/v) Compound ofExample 5 0.10 Benzalkonium chloride 0.01 EDTA 0.05Hydroxyethylcellulose (NATROSOL M) 0.50 Sodium metabisulfite 0.10 Sodiumchloride (0.9%) q.s. Total = 100.0

[0403] (Other compounds having a structure according to Formula (I) areused with substantially similar results.)

[0404] A human male subject weighing 90 kg (198 lbs), suffering fromcorneal ulcerations, is treated by a method of this invention.Specifically, for 2 months, a saline solution containing 10 mg of thecompound of Example 5 is administered to said subject's affected eyetwice-daily.

Example G

[0405] A composition for parenteral administration is made comprising:Component Amount The compound of Example 4 100 mg/ml carrier Carrier:sodium citrate buffer with (percent by weight of carrier): lecithin0.48% carboxymethylcellulose 0.53 povidone 0.50 methyl paraben 0.11propyl paraben 0.011

[0406] The above ingredients are mixed, forming a suspension.Approximately 2.0 ml of the suspension is administered, via injection,to a human subject with a premetastatic tumor. The injection sitejuxtaposes the tumor. This dosage is repeated twice daily, forapproximately 30 days. After 30 days, symptoms of the disease subside,and dosage is gradually decreased to maintain the patient.

[0407] (Other compounds having a structure according to Formula I areused with substantially similar results.)

Example H

[0408] A mouthwash composition is prepared; Component % w/v The compoundof Example 1 3.00 SDA 40 Alcohol 8.00 Flavor 0.08 Emulsifier 0.08 SodiumFluoride 0.05 Glycerin 10.00 Sweetener 0.02 Benzoic acid 0.05 Sodiumhydroxide 0.20 Dye 0.04 Water balance to 100%

[0409] A patient with gum disease uses 1 ml of the mouthwash thricedaily to prevent further oral degeneration.

[0410] (Other compounds having a structure according to Formula I areused with substantially similar results.)

Example I

[0411] A lozenge composition is prepared; Component % w/v The compoundof Example 3 0.01 Sorbitol 17.50 Mannitol 17.50 Starch 13.60 Sweetener1.20 Flavor 11.70 Color 0.10 Corn syrup balance to 100%

[0412] A patient uses the losenge to prevent loosening of an implant inthe maxilla. (Other compounds having a structure according to Formula Iare used with substantially similar results.)

Example J

[0413] Chewing Gum Composition Component w/v % The compound of Example 10.03 Sorbitol crystals 38.44 Paloja-T gum base* 20.00 Sorbitol (70%aqueous solution) 22.00 Mannitol 10.00 Glycerine 7.56 Flavor 1.00

[0414] A patient chews the gum to prevent loosening of dentures.

[0415] (Other compounds having a structure according to Formula I areused with substantially similar results.)

Example K

[0416] Components w/v % Compound of example 25 4.0 USP Water 50.656Methylparaben 0.05 Propylparaben 0.01 Xanthan Gum 0.12 Guar Gum 0.09Calcium carbonate 12.38 Antifoam 1.27 Sucrose 15.0 Sorbitol 11.0Glycerin 5.0 Benzyl Alcohol 0.2 Citric Acid 0.15 Coolant 0.00888 Flavor0.0645 Colorant 0.0014

[0417] The composition is prepared by first mixing 80 kg of gylcerin andall of the benzyl alcohol and heating to 65 C, then slowly adding andmixing together methylparaben, propylparaben, water, xanthan gum, andguar gum. Mix these ingredients for about 12 minutes with a Silversonin-line mixer. Then slowly add in the following ingredients in thefollowing order: remaining glycerin, sorbitol, antifoam C, calciumcarbonate, citric acid, and sucrose. Separately combine flavors andcoolants and then slowly add to the other ingredients. Mix for about 40minutes.

[0418] The patient takes the formulation to prevent flare up of colitis.

[0419] All references described herein are hereby incorporated byreference.

[0420] While particular embodiments of the subject invention have beendescribed, it will be obvious to those skilled in the art that variouschanges and modifications of the subject invention can be made withoutdeparting from the spirit and scope of the invention. It is intended tocover, in the appended claims, all such modifications that are withinthe scope of this invention.

What is claimed is:
 1. A compound having a structure according toFormula (I)

wherein Ar is alkyl, heteroalkyl, aryl or heteroaryl, substituted orunsubstituted; R₁ is OH, alkoxy, NHOR₂, where R₂ is hydrogen or alkyl; Wis one or more of hydrogen, lower alkyl or an allkylene bridge; Y isindependently one or more of hydroxy, SR₃, SOR₄, SO₂R₅, alkoxy, amino,wherein amino is of formula NR₆,R₇, wherein R₆ and R₇ are independentlychosen from hydrogen, alkyl, heteroalkyl, heteroaryl, aryl, OR₃, SO₂R₈,COR₉, CSR₁₀, PO(R₁₁)₂; and R₃ is hydrogen, alkyl, aryl, heteroaryl; R₄is alkyl, aryl, heteroaryl; R₈ is alkyl, aryl, heteroaryl, heteroalkyl,amino, alkylamino, dialkylamino, arylamino, diarylamino andalkylarylamino; R₉ is hydrogen, alkoxy, aryloxy, heteroaryloxy, alkyl,aryl, heteroaryl, heteroalkyl, amino, alkylamino, dialkylamino,arylamino and alkylarylamino; R₁₀ is alkyl, aryl, heteroaryl,heteroalkyl, amino, alkylamino, dialkylamino, arylamino, diarylamino andalkylarylamino; R₁₁ is alkyl, aryl, heteroaryl, heteroalkyl; Z ishydrogen, hydroxy, alkyl, alkylene or heteroalkylene; n is 1-3 and anoptical isomer, diastereomer or enantiomer for formula (I), or apharmaceutically-acceptable salt, or biohydrolyzable amide, ester, orimide thereof.
 2. A compound according to claim 1 where, e compound isof structure:

wherein Ar is aryl or heteroaryl, substituted or unsubstituted; R₁ isOH, alkoxy, NHOR₂, where R₂ is hydrogen or alkyl; W is one or more ofhydrogen, lower alkyl; Y is independently one or more of hydroxy, SR₃,SOR₄, SO₂R₈, alkoxy, amino, wherein amino is of formula NR₆,R₇, whereinR₆ and R₇ are independently chosen from hydrogen, alkyl, heteroalkyl,heteroaryl, aryl, OR₃, SO₂R₈, COR₉, CSR₁₀, PO(R₁₁)₂; and R₃ is hydrogen,alkyl, aryl, heteroaryl; R₄ is alkyl, aryl, heteroaryl; R₈ is alkyl,aryl, heteroaryl, heteroalkyl, amino, alkylamino, dialkylamino,arylamino, diarylamino and alkylarylamino; R₉ is hydrogen, alkoxy,aryloxy, heteroaryloxy, alkyl, aryl, heteroaryl, heteroalkyl, amino,alkylamino, dialkylamino, arylamino and alkylarylamino; R₁₀ is alkyl,aryl, heteroaryl, heteroalkyl, amino, alkylamino, dialkylamino,arylamino, diarylamino and alkylarylamino; R₁₁ is alkyl, aryl,heteroaryl, heteroalkyl; Z is hydrogen; n is 1-3, and an optical isomer,diastereomer or enantiomer for Formula (I), or apharmaceutically-acceptable salt, or biohydrolyzable amide, ester, orimide thereof.
 3. The compound of claim 2, wherein Ar is phenyl orsubstituted phenyl.
 4. The compound of claim 3, wherein Ar issubstituted phenyl and the substitution is with hydroxy, alkoxy, nitroor halo.
 5. The compound of claim 4, wherein Ar is substituted withmethoxy, bromo, nitro and butoxy.
 6. The compound of claim 5, wherein Aris substituted at the ortho or para position relative to the sulfonyl.7. The compound of claim 3, wherein Y is independently one or more ofhydroxy, SO₂R₅, alkoxy, amino, wherein amino is of formula NR₆,R₇,wherein R₆ and R₇ are independently chosen from hydrogen, alkyl,heteroalkyl, heteroaryl, aryl, SO₂R₈, COR₉.
 8. The compound of claim 7,wherein Y is independently one or more of hydroxy, amino, wherein aminois of formula NR₆,R₇, wherein R₆ and R₇ are independently chosen fromhydrogen, alkyl, heteroalkyl, heteroaryl, aryl, SO₂R₈, COR₉.
 9. Acompound according to claim 1 wherein the compound is chosen from;(1N)-(4-Methoxyphenylsulfonyl)-(2R)-N-hydroxycarboxamido-(4S)-hydroxypyrrolidine;((1N)-(4-Methoxyphenylsulfonyl)-(2R)-N-hydroxycarboxamido-(4S)-hydroxypyrrolidine;(1N)-(4-Methoxyphenylsulfonyl)-(2S)-N-hydroxycarboxamido-(4R)-hydroxypyrrolidine;(1N)-(4-Methoxyphenylsulfonyl)-(2S)-N-hydroxycarboxamido-(4S)-hydroxypyrrolidine;(1N)-(4-Methoxyphenylsulfonyl)-(2R)-N-hydroxycarboxamido-(4S)-methoxypyrrolidine;(1N)-(4-Methoxyphenylsulfonyl)-(2R)-N-hydroxycarboxamido-(4S)-(2-mercapto-benzothiazolyl)-pyrrolidine;(1N)-(4-Methoxyphenylsulfonyl)-(2R)-N-hydroxycarboxamido-(4R)-(2-mercaptobenzo-thiazolyl)-pyrrolidine;(1N)-(4-Methoxyphenylsulfonyl)-(2R)-N-hydroxycarboxamido-(4S)-[(1N)-methyl-2-mercaptoimidazyl]-pyrrolidine;(1N)-(4-Methoxyphenylsulfonyl)-(2R)-N-hydroxycarboxamido-(4R)-[(1N)-methyl-2-mercaptoimidazyl]-pyrrolidine;(1N)-(4-Methoxyphenylsulfonyl)-(2R)-N-hydroxycarboxamido-(4S)-phenoxypyrrolidine;(1N)-(4-Methoxyphenylsulfonyl)-(2R)-N-hydroxycarboxamido-(4S)-(4-benzyloxy)-phenoxypyrrolidine;(1N)-4-Methoxyphenylsulfonyl-(2R)-N-hydroxycarboxamido-(4S)-(3-N-phenylamino)-phenoxylpyrrolidine;(1N)-4-Methoxyphenylsulfonyl-(2R)-N-hydroxycarboxamido-(4S)-phenoxypyrrolidine;(1N)-4-Methoxyphenylsulfonamido-(2R)-N-hydroxycarboxamido-(4S)-mercaptophenylpyrrolidine;(1N)-(4-Methoxyphenylsulfonyl)-(2R)-N-hydroxycarboxamido-(4S)-(4-methoxyphenyl-thioloxy)-pyrrolidine;(1N)-(4-Methoxyphenylsulfonyl)-(2R)-N-hydroxycarboxamido-(4S)-(3-methoxy-mercaptophenyl)-pyrrolidine;(1N)-(4-Methoxyphenylsulfonyl)-(2R)-N-hydroxycarboxamido-(4S)-(^(n)hexylamino)-pyrrolidine;(1N)-(4-Methoxyphenylsulfonyl)-(2R)-hydroxycarboxamido-(4S)-thiopyrrolidine;(±)-(1N)-(4-Methoxyphenylsulfonyl)-(2R)-N-hydroxycarboxamido-(3S)-phenylpyrrolidine;(1N)-(4-Methylphenylsulfonyl)-(2R)-N-hydroxycarboxamido-(4S)-hydroxypyrrolidine;(1N)-(3,4-Dimethoxyphenylsulfonyl)-(2R)-N-hydroxycarboxamido-(4R)-hydroxypyrrolidine;(1N)-(2-Nitro-4-methoxyphenylsulfonyl)-(2R)-N-hydroxycarboxamido-(4R)-hydroxypyrrolidine;(1N)-4-^(n)Butoxyphenylsulfonamido-(2R)-N-hydroxycarboxamido-(4R)-hydroxypyrrolidine;(1N)-(4-^(n)Butoxyphenylsulfonyl)-(2R)-N-hydroxycarboxamido-(4S)-hydroxypyrrolidine;(1N)-(4-^(n)Butoxyphenylsulfonyl)-(2R)-N-hydroxycarboxamido-(4S)-(2-mercapto-benzothiazolyl)-pyrrolidine;(1N)-(2-Nitro-4-methoxyphenylsulfonyl)-(2R)-N-hydroxycarboxamido-(4S)-(2-mercapto-benzothiazolyl)-pyrrolidine;(1N)-(4-Methoxyphenylsulfonyl)-(2R)-N-hydroxycarboxamido-(4S)-(4-methoxyphenyl-thioloxy)-pyrrolidine;(±)-(1N)-4-Methoxyphenylsulfonyl-(2R)-N-hydroxycarboxamido-5-pyrrolidinone;and(1N)-4-Methoxyphenylsulfonyl-(2R)-hydroxy-carboxamido-(4,4R)-hydroxy-ethylpyrrolidine.10. A pharmaceutical composition comprising: (a) a safe and effectiveamount of a compound of claim 1; and (b) a pharmaceutically-acceptablecarrier.
 11. A pharmaceutical composition comprising: (a) a safe andeffective amount of a compound of claim 2; and (b) apharmaceutically-acceptable carrier.
 12. A pharmaceutical compositioncomprising: (a) a safe and effective amount of a compound of claim 3;and (b) a pharmaceutically-acceptable carrier.
 13. A pharmaceuticalcomposition comprising: (a) a safe and effective amount of a compound ofclaim 7; and (b) a pharmaceutically-acceptable carrier.
 14. A method forpreventing or treating a disease associated with unwantedmetalloprotease activity in a mammalian subject, the method comprisingadministering to said subject a safe and effective amount of a compoundof claim
 1. 15. A method for preventing or treating a disease associatedwith unwanted metalloprotease activity in a mammalian subject, themethod comprising administering to said subject a safe and effectiveamount of a compound of claim
 2. 16. A method for preventing or treatinga disease associated with unwanted metalloprotease activity in a humanor other animal subject, the method comprising administering to saidsubject a safe and effective amount of a compound of claim
 3. 17. Amethod for preventing or treating a disease associated with unwantedmetalloprotease activity in a mammalian subject, the method comprisingadministering to said subject a safe and effective amount of a compoundof claim
 7. 18. A method for preventing or treating a disorder modulatedby metalloproteases, wherein the disorder is chosen from the groupcomprising, arthritis, cancer, cardiovascular disorders, skin disordersocular disorders, inflammation and gum disease by administering to amammal in need of such treatment, a safe and effective amount of ametalloprotease inhibitor according to claim
 1. 19. A method forpreventing or treating a disorder according to claim 18, wherein thedisorder is arthritis, and is chosen from the group comprising,osteoarthritis and rheumatoid arthritis.
 20. A method for preventing ortreating a disorder according to claim 18, wherein the disorder iscancer, and the treatment prevents or arrests tumor growth andmetastasis.
 21. A method for the preventing or treating a disorderaccording to claim 18, wherein the disorder is a cardiovascular disorderchosen from the group compromising dilated cardiomyopathy, congestiveheart failure, atherosclerosis, plaque rupture, reperfusion injury,ischemia, chronic obstructive pulmonary disease, angioplasty restenosisand aortic aneurysm.
 22. A method for the preventing or treating adisorder according to claim 18, wherein the disorder is an oculardisorder, and is chosen from the group comprising, corneal ulceration,lack of corneal healing, macular degeneration, and pterygium.
 23. Amethod for preventing or treating a disorder according to claim 18,wherein the disorder is gum disease, and is chosen from the groupcomprising, periodontal disease, and gingivitis.
 24. A method forpreventing or treating a disorder, according to claim 18, wherein thedisorder is skin disorder chosen from the group comprising wrinklerepair and prevention, U.V. skin damage, epidermolysis bullosa,psoriasis, sclerodema, atopic dermatitis and scarring.
 25. A method forpreventing the loosening of prosthetic devices chosen from the groupcomprising joint replacements and dental prosthesis by administering toa mammal in need of such treatment, a safe and effective amount of ametalloprotease inhibitor according to claim
 1. 26. A method fortreating inflammatory conditions according to claim 18, chosen from thegroup comprising inflammatory bowel disease, Crohn's Disease, ulcerativecolitis, pancreatitis, diverticulitis, acne inflammation, bronchitis,arthritis, asthma.
 27. A method of treating multiple sclerosis,comprising administering to a mammal in need of such treatment, a safeand effective amount of a metalloprotease inhibitor according toclaim
 1. 28. A method for treating musculoskeletal disease or cachexiacomprising administering to a mammal in need of such treatment, a safeand effective amount of a metalloprotease inhibitor according to claim1.